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Description
Description
The second module in Cloning and Sequencing Explorer Series, the GAPDH PCR Module allows students to amplify the GAPC gene from a plant. Materials include primers, PCR master mix, controls, exonuclease, molecular weight ruler, loading dye, sterile water, test tubes/holders, and curriculum. This product is for education use only.
Specifications
Specifications
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Initial GAPDH PCR primers, 50 µl
1
Nested GAPDH PCR primers, 50 µl
1
PCR master mix, 1.2 ml
3
Plasmid control DNA, 1 ml
1
Control Arabidopsis genomic DNA, 20 µl
1
Exonuclease I, 50 µl
1
500 bp molecular weight ruler, 400 µl
1
Orange G loading dye, 1 ml
2
Sterile water, 2.5 ml
3
PCR tubes, 0.2 ml
150
Capless PCR tube adaptors, 1.5 ml
150
Colored microcentrifuge tubes, 2.0 ml
120
Jellyfish-shaped foam floats
12
Curriculum, including teacher's guide, student manual, and graphic quick guide
1