Cloning and Sequencing Explorer Series #1665000edu

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Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only

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To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.</p> <p class="headerClassText">How do I apply for the education discount?</p> <p class="descClassText">If you are an educator at the high school or college level, visit our <a href="/en-us/education/education-discount-policy?ID=MRFKWO4VY">Education Discount Policy</a> page to establish an education account number.</p> </div> /webroot/web/html EDU_price_overlay.html /webroot/web/html/EDU_price_overlay.html sandeep Edu Edu Price Overlay Edu Price Overlay Content 07/30/13 03:25 AM 07/30/20 05:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE N 0 1665000EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jc99dt6jjc99dt6k" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc99dt6jjc99dt6k"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="20" Minute="34" Second="32" ZoneOffsetMillis="0" Time="1515702872122">Thu Jan 11 20:34:32 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5000EDU_0921121012345.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="20" Minute="34" Second="32" ZoneOffsetMillis="0" Time="1515702872122">Thu Jan 11 20:34:32 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODWEST"> <Deployment Id="StandardMetadata.jc99dt9rjc99dt9s" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc99dt9rjc99dt9s"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODWEST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="14" Second="23" ZoneOffsetMillis="0" Time="1515708863686">Thu Jan 11 22:14:23 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5000EDU_0921121012345.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="14" Second="23" ZoneOffsetMillis="0" Time="1515708863686">Thu Jan 11 22:14:23 UTC 2018</LastExecutionTime> </Node> <Node Name="PRODEAST"> <Deployment Id="StandardMetadata.jc99dt9rjc99dt9y" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jc99dt9rjc99dt9y"> <Name>StandardMetadata</Name> <TargetEnviroment>PRODEAST</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="34" Second="1" ZoneOffsetMillis="0" Time="1515710041962">Thu Jan 11 22:34:01 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5000EDU_0921121012345.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="1" DayOfMonth="11" DayOfWeek="4" Year="2018" Hour="22" Minute="34" Second="1" ZoneOffsetMillis="0" Time="1515710041962">Thu Jan 11 22:34:01 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM en_US jc99dsny 2017-11-14 07:07:14 true true internet/product_SKU LSE N en 09/21/19 12:10 PM 1515711331947 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:14 1665000edu-cloning-sequencing-explorer-series 1665000EDU Cloning and Sequencing Explorer Series Cloning and Sequencing Explorer Series Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum; education use only Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5000EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5000EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5000EDU_feature.jpg preview RepEx 166-5000EDU Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only 1665000EDU, 1665000, cloning, sequencing, dna sequencing, gene sequencing, explorer, molecular biology, bioinformatics, lab, dna extraction, dna purification, education, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 <p>Use the Cloning and Sequencing Explorer series to give your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the <em>GAPC</em> gene from a plant of their choice. <em>GAPC</em> is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.</p> <p>The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:</p> <ol> <li>Extract genomic DNA from plants</li> <li>Amplify a <em>GAPDH</em> gene using PCR and analyze using electrophoresis</li> <li>Purify the PCR product</li> <li>Ligate the PCR product into a plasmid</li> <li>Transform the ligated plasmid into bacteria</li> <li>Grow minipreps of the transformed bacteria</li> <li>Isolate plasmid DNA and analyze by restriction digestion</li> <li>Sequence plasmid DNA (sequencing service not included)</li> <li>Analyze <em>GAPDH</em> gene sequence using bioinformatics</li> </ol> <h2 class="descHeader">Features and Benefits</h2> <ul> <li>6&ndash;8 week series of laboratory activities made up of eight modular kits</li> <li>Provides sufficient materials for 12 student workstations, up to four students per workstation</li> <li>Sequencing service not included</li> </ul> <p>For more information and resources, visit the <a href="/en-us/product/cloning-sequencing-explorer-series">Cloning and Sequencing Explorer Series</a> product page.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/nucleic-acid-extraction-module">Nucleic Acid Extraction Module</a></li> <li><a href="/en-us/product/gapdh-pcr-module">GAPDH PCR Module</a></li> <li><a href="/en-us/product/electrophoresis-module">Electrophoresis Module</a></li> <li><a href="/en-us/product/pcr-kleen-spin-purification-module">PCR Kleen&trade; Spin Purification Module</a></li> <li><a href="/en-us/product/ligation-transformation-module">Ligation and Transformation Module</a></li> <li><a href="/en-us/product/microbial-culturing-module">Microbial Culturing Module</a></li> <li><a href="/en-us/product/aurum-plasmid-mini-purification-module">Aurum&trade; Plasmid Mini Purification Module</a></li> <li><a href="/en-us/product/sequencing-bioinformatics-module">Sequencing and Bioinformatics Module</a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li><a href="/webroot/web/pdf/lse/literature/10000069187.pdf" target="_blank">Bio-Rad Explorer&trade; Cloning and Sequencing Explorer Series Curriculum Manual</a><br /> <a href="/webroot/web/pdf/lse/literature/Bulletin_6858.pdf" target="_blank"></a></li> </ul> /webroot/web/html/lse/products/SKUs/autopopulated 1665000EDU-desc.html /webroot/web/html/lse/products/SKUs/autopopulated/1665000EDU-desc.html 10000053330 templatedata/internet/documentation/data/LSE/Global/English/Literature/10000053330_1453418783_en_XX.xml false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665000edu&CartQuote=QT&quantity= true true false $2,673.00 true %242%2C138.40 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665000edu&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665000edu&quantity= 166-5000-MSDS Cloning & Sequencing Explorer Series Kit Korea Korean /webroot/web/pdf/WWMSDS/LSGC/ROK/ROK_KOR_1665000.pdf Canada French /webroot/web/pdf/WWMSDS/LSGC/FCN/FCN_FR_1665000.pdf The Netherlands Dutch /webroot/web/pdf/WWMSDS/LSGC/NL/NL_NL_1665000.pdf Hungary English /webroot/web/pdf/WWMSDS/LSGC/HE/HE_ENG_1665000.pdf Hungary Hungarian /webroot/web/pdf/WWMSDS/LSGC/H/H_UNG_1665000.pdf Poland Polish /webroot/web/pdf/WWMSDS/LSGC/PL/PL_POL_1665000.pdf Hong Kong English /webroot/web/pdf/WWMSDS/LSGC/HK/HK_ENG_1665000.pdf Italy Italian /webroot/web/pdf/WWMSDS/LSGC/I/I_IT_1665000.pdf Portugal Portuguese /webroot/web/pdf/WWMSDS/LSGC/P/P_PT_1665000.pdf Canada English /webroot/web/pdf/WWMSDS/LSGC/CDN/CDN_USA_1665000.pdf true USA English /webroot/web/pdf/WWMSDS/LSGC/USA/USA_USA_1665000.pdf China Chinese /webroot/web/pdf/WWMSDS/LSGC/RC/RC_ZH_1665000.pdf Spain Spanish /webroot/web/pdf/WWMSDS/LSGC/E/E_ES_1665000.pdf Sweden Swedish /webroot/web/pdf/WWMSDS/LSGC/S/S_S_1665000.pdf Singapore English /webroot/web/pdf/WWMSDS/LSGC/SGP/SGP_ENG_1665000.pdf United Kingdom English /webroot/web/pdf/WWMSDS/LSGC/GB/GB_ENG_1665000.pdf Austria English /webroot/web/pdf/WWMSDS/LSGC/AE/AE_ENG_1665000.pdf Sweden English /webroot/web/pdf/WWMSDS/LSGC/SE/SE_ENG_1665000.pdf New Zealand English /webroot/web/pdf/WWMSDS/LSGC/NZ/NZ_ENG_1665000.pdf Switzerland German /webroot/web/pdf/WWMSDS/LSGC/CH/CH_D_1665000.pdf China English /webroot/web/pdf/WWMSDS/LSGC/PRC/PRC_ENG_1665000.pdf Italy English /webroot/web/pdf/WWMSDS/LSGC/IE/IE_ENG_1665000.pdf India English /webroot/web/pdf/WWMSDS/LSGC/IN/IN_ENG_1665000.pdf Switzerland English /webroot/web/pdf/WWMSDS/LSGC/CHE/CHE_ENG_1665000.pdf Korea English /webroot/web/pdf/WWMSDS/LSGC/KOR/KOR_ENG_1665000.pdf Israel English /webroot/web/pdf/WWMSDS/LSGC/IL/IL_ENG_1665000.pdf Poland English /webroot/web/pdf/WWMSDS/LSGC/POL/POL_ENG_1665000.pdf The Netherlands English /webroot/web/pdf/WWMSDS/LSGC/NLE/NLE_ENG_1665000.pdf Austria German /webroot/web/pdf/WWMSDS/LSGC/A/A_D_1665000.pdf Switzerland French /webroot/web/pdf/WWMSDS/LSGC/CHF/CHF_FR_1665000.pdf Norway Norwegian /webroot/web/pdf/WWMSDS/LSGC/N/N_N_1665000.pdf
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Use the Cloning and Sequencing Explorer series to give your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the GAPC gene from a plant of their choice. GAPC is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.

The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:

  1. Extract genomic DNA from plants
  2. Amplify a GAPDH gene using PCR and analyze using electrophoresis
  3. Purify the PCR product
  4. Ligate the PCR product into a plasmid
  5. Transform the ligated plasmid into bacteria
  6. Grow minipreps of the transformed bacteria
  7. Isolate plasmid DNA and analyze by restriction digestion
  8. Sequence plasmid DNA (sequencing service not included)
  9. Analyze GAPDH gene sequence using bioinformatics

Features and Benefits

  • 6–8 week series of laboratory activities made up of eight modular kits
  • Provides sufficient materials for 12 student workstations, up to four students per workstation
  • Sequencing service not included

For more information and resources, visit the Cloning and Sequencing Explorer Series product page.

Related Products

Supporting Documents

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Cloning & Sequencing Explorer Series Kit  (SDS)gene

Bulletin# 166-5000-MSDS

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