ProteoMiner™ Protein Enrichment Introductory Large-Capacity Kit #1633009

ProteoMiner™ Protein Enrichment Introductory Large-Capacity Kit

2 preps, low-abundance protein enrichment kit for 50 mg of total protein, includes 2 spin columns, wash buffer, elution reagents, collection tubes

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Number Description Options
163-3006-MSDS ProteoMiner Protein Enrichment Kit English

Assess enrichment of proteins in your sample using the ProteoMiner Protein Enrichment Introductory Large-Capacity Kit to simultaneously concentrate low-abundance proteins and lower the concentration of high-abundance proteins in ≥50 mg of sample. The protein complexity of the sample is maintained.

The enrichment protocol uses a large, highly diverse library of combinatorial peptide ligands bound to beads. After high-abundance proteins have saturated all their high-affinity ligands, the unbound excess protein is washed away. Most medium- and low-abundance proteins will not saturate their ligands, resulting in no excess to be washed away.

  • Knowledge of the identity and characteristics of the proteins within a sample is not required
  • Compatible with a wide range of sample types
  • Narrows the range of protein concentrations within a sample
  • Can be used for differential expression analysis
  • Compatible with most downstream applications including 1-D and 2-D electrophoresis and mass spectrometry

For fractionation of the protein sample after binding to the beads, the ProteoMiner Protein Enrichment Introductory Large-Capacity Kit can be used with the ProteoMiner Sequential Elution Reagents (1633003). The elution kit contains four different elution buffers, providing multiple options for successive elution of populations of proteins with different characteristics. For larger numbers of samples the ProteoMiner Protein Enrichment Large-Capacity Kit (1633007) and Sequential Elution Reagents can be purchased together in one kit, the ProteoMiner Sequential Elution Large-Capacity Kit (1633011). Sequential elution has been optimized for samples for SELDI. Elution reagents are not compatible with direct use of fractions in 2-D gel electrophoresis.

Kit Contents

  • Spin columns, 2;  containing 100 µl bead slurry (20% beads, 20% v/v EtOH), 20 µl settled bead volume
  • Wash Buffer, 150 mM NaCl, 10 mM NaH2PO4, pH 7.4, 10 ml
  • Elution Reagent, lyophilized 8 M urea, 2% CHAPS, 2 vials
  • Rehydration Reagent, 5% acetic acid, 1 ml
  • Capless 2 ml centrifuge tubes, 4
  • Capped 2 ml centrifuge tubes, 2
  • Instruction Manual
  • Quick Start Guide

Product Options

ProteoMiner Protein Enrichment and Elution Kits Capacity Number of Samples Catalog Number
Small-Capacity Enrichment Kit ≥10 mg 10 1633006
Introductory Small-Capacity Enrichment Kit ≥10 mg 2 1633008
Large-Capacity Enrichment Kit ≥50 mg 10 1633007
Introductory Large-Capacity Enrichment Kit ≥50 mg 2 1633009
Sequential Elution Reagents   10 1633003
Small-Capacity Elution Kit (combines 1633006 and 2633003) ≥10 mg 10 1633010
Large-Capacity Elution Kit (combines 1633007 and 2633003) ≥50 mg 10 1633011

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Supporting Documentation

  • ProteoMiner Protein Enrichment and Sequential Kit Protocols
  • ProteoMiner Sequential Elution Kit Protocol
  • ProteoMiner Protein Enrichment Kits Instruction Manual
  • ProteoMiner Protein Enrichment Technology Brochure
  • Enrichment of Medium- and Low-Abundance Proteins in Sample Types Using ProteoMiner Technology Application Note