Description
Description
Choose the ReadyPrep Protein Extraction Kit (Signal) to enrich for signaling proteins. This kit uses differential solubilization at 4°C in the presence of Triton X-100 to isolate proteins associated with lipid rafts.
- Isolate proteins involved in intracellular membrane trafficking and signaling pathways
- After fractionation, proteins are easily solubilized in the supplied buffer
- Solubilized proteins can be used immediately in IEF, SDS-PAGE and other applications
Many proteins located within lipid rafts are resistant to solubilization at 4°C by Triton X-100 and other nonionic detergents. The ReadyPrep Protein Extraction Kit (Signal) utilizes a proprietary mixture of nonionic detergents to extract and remove hydrophilic and soluble proteins, leaving behind the signal proteins in a detergent-insoluble fraction.
Proteins that can be isolated using this kit include
- Gycosylphosphatidylinositol (GPI)-anchored proteins
- Caveolin and other caveolae-associated proteins
- Acylated tyrosine kinases
- Heterotrimeric G-proteins
- Some proteins with transmembrane domains
Kit Components
For 50 extractions of 25–50 mg of cells or tissue:
- Signal protein extraction buffer 1 (S1), 50 ml
- Signal protein extraction buffer 2 (S2), 50 ml
- Protein solubilization buffer (PSB), 25 g
- PSB diluent, 30 ml
- Instruction manual
Bio-Rad offers a range of ReadyPrep Kits for Fractionation by Cellular Location. These kits reduce sample complexity, increase detection and identification of low-abundance proteins, and simplify proteomic studies.
ReadyPrep Kit for Fractionation by Cellular Location | Proteins Targeted | Catalog Number |
ReadyPrep Protein Extraction Kit (Cytoplasmic/Nuclear) | Cytoplasmic and nuclear | 1632089 |
ReadyPrep Protein Extraction Kit (Membrane I) | Membrane-anchored, with one or two transmembrane domains, and hydrophobic | 1632088 |
ReadyPrep Protein Extraction Kit (Membrane II) | More than two transmembrane domains and/or proteins resistant to Membrane I Kit | 1632084 |
ReadyPrep Protein Extraction Kit (Signal) | Associated with lipid rafts | 1632087 |
Related Products
- ReadyPrep Kits for Fractionation by Solubility
- Aurum™ Ion Exchange Mini Kits for Fractionation by Charge
- Rotofor® Liquid-Phase IEF Cells for Fractionation by pI
- Model 491 Prep Cell and Mini Prep Cell for Fractionation by Solubility
- ReadyPrep™ 2-D Starter Kit
- PROTEAN® i12™ IEF System
- ReadyStrip™ IPG Strips
- ReadyPrep Reduction-Alkylation Kit (1632090)
- PowerPac™ HV High-Voltage Power Supply
Related Categories
- Protein Gels
- Electrophoresis Chambers
- 2-D Electrophoresis Buffers and Reagents
- Ampholytes
- Tube Gel IEF Systems
- Gel Imaging Systems
- 2-D Electrophoresis Workflow
Supporting Documentation
- ReadyPrep Protein Extraction Kit (Signal) Instruction Manual
- Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure
- ReadyStrip IPG Strip Instruction Manual
- PROTEAN i12 IEF System Brochure
- Host Cell Protein (HCP) Workflow Quick Guide