10x Tris/Glycine Buffer for Western Blots and Native Gels #1610734

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10x Tris/Glycine Buffer for Western Blots and Native Gels

1 L, 10x premixed electrophoresis buffer

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Number Description Options
161-0771-MSDS 10X Tris/Glycine Buffer English
French

Use 10x Tris/Glycine Buffer as a transfer buffer for western blots or as a running buffer for native protein gel electrophoresis.

  • For tank or semi-dry blotting for SDS PAGE gels, usually with the addition of 20% methanol
  • For tank blotting of native gels, without methanol
  • As a running buffer for native gels

Precast gels that can be used for native electrophoresis

Precast Gel Type Size Gel Chemistry
Mini-PROTEAN® TGX™ Mini Laemmli-like, long shelf life, fast separation with high resolution
Criterion™ TGX™ Midi
Mini-PROTEAN® TGX Stain-Free™ Mini Laemmli-like, long shelf life, fast separation with high resolution, unique trihalo compounds for rapid fluorescent protein detection
Criterion™ TGX Stain-Free™ Midi
Criterion™ Tris-HCl Midi Standard Laemmli
Criterion Stain Free™ Tris-HCl Midi Standard Laemmli, unique trihalo compounds for rapid fluorescent protein detection
Criterion XT Tris-Acetate Midi Discontinuous buffer ion fronts form moving boundaries to stack, and then separate proteins

Note: Most proteins have an acidic or slightly basic pI (~3–8) and are run with the power supply connected to the electrophoresis chamber as for SDS-PAGE. If more basic proteins (pl >8.5) are of interestbeing separated, change the polarity of the electrodes, since they have a net positive charge.

Packaging Options

10x Buffer Volume Catalog Number
1 L 1610734
5 L cube 1610771

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