Profinity™ IMAC Resin, Ni-charged, 100 ml #1560135


Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

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Ligand Iminodiacetic acid (IDA)
Form 50% slurry in 20% ethanol, Ni-charged
Particle size 45–90 μm (mean 60 μm)
Capacity 12–30 µmol Cu2+/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein)
Maximum operating pressure 109 psi (7.5 bar)
Recommended flow rate ≤600 cm/hr at 25°C
Operating temperature range 4–40°C
Autoclavability (121°C, 30 min) Yes (in 0.1 M sodium acetate)
Storage 4°C or ambient
Shelf life ≥1 year at ambient temperature in 20% ethanol
Number Description Options
156-0131-MSDS Profinity™ IMAC Nickel Charged Resin English

Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.

  • Chelating ligand is iminodiacetic acid (IDA)
  • Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
  • Stable across a wide pH range (1–14)
  • Works under native or denaturing conditions
  • Can be used for single-step purification from crude samples or contaminant removal

Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.

Product Options

Resin Form Package Size Catalog Number
Ni-charged 10 ml 1560131
Ni-charged 25 ml 1560133
Ni-charged 100 ml 1560135
Ni-charged 500 ml 1560137
Uncharged 10 ml 1560121
Uncharged 50 ml 1560123
Uncharged 500 ml 1560125
Uncharged 1 L 1560127

Prepacked Bio-Scale™ Mini Profinity™ IMAC Cartridges with uncharged or Ni-charged IDA, and Bio-Scale™ Mini Nuvia™ IMAC Cartridges with Ni-charged NTA are available.

Related Products

  • Native IMAC Buffer Kit (6200239) includes IMAC lysis, wash, and elution buffers
  • Native IMAC Purification Kits, 1 ml (6200241) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (6200242) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge
  • Nuvia IMAC Resin, Ni-charged nitrilotriacetic acid (NTA)
  • Empty Bio-Scale Mini Cartridges, 1 ml (7324660) and 5 ml (7324661)
  • Poly-Prep® Chromatography Columns, hold up to 2 ml support
  • His Antibody (6200203)

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