Profinity™ IMAC Resin, uncharged, 10 ml #1560121

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Overview

Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

Safety Data Sheet

Safety Data Sheet

Description

Description

Use Profinity IMAC Resin for purification of recombinant histidine-tagged proteins. Optimized ligand density and open pore structure provides high-stringency binding of his-tagged and metal-binding proteins for a wide range of molecular weights.

  • Chelating ligand is iminodiacetic acid (IDA)
  • Can be used in batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems
  • Stable across a wide pH range (1–14)
  • Works under native or denaturing conditions
  • Can be used for single-step purification from crude samples or contaminant removal

Profinity IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.

Product Options

Resin Form Package Size Catalog Number
Ni-charged 10 ml 1560131
Ni-charged 25 ml 1560133
Ni-charged 100 ml 1560135
Ni-charged 500 ml 1560137
Uncharged 10 ml 1560121
Uncharged 50 ml 1560123
Uncharged 1 L 1560127

Prepacked Bio-Scale™ Mini Profinity™ IMAC Cartridges with uncharged or Ni-charged IDA, and Bio-Scale™ Mini Nuvia™ IMAC Cartridges with Ni-charged NTA are available.

Related Products

  • Native IMAC Buffer Kit (6200239) includes IMAC lysis, wash, and elution buffers
  • Native IMAC Purification Kits, 1 ml (6200241) includes Native IMAC buffer Kit and 2x1 ml Bio-Scale Mini IMAC Cartridges, and 5 ml (6200242) includes Native IMAC buffer Kit and 1x5 ml Bio-Scale Mini IMAC Cartridge
  • Nuvia IMAC Resin, Ni-charged nitrilotriacetic acid (NTA)
  • Empty Bio-Scale Mini Cartridges, 1 ml (7324660) and 5 ml (7324661)
  • Poly-Prep® Chromatography Columns, hold up to 2 ml support
  • His Antibody (6200203)

Related Categories

Supporting Documents

Online Resources

Specifications

Specifications

Ligand
Iminodiacetic acid (IDA)
Form
50% slurry in 20% ethanol, uncharged
Particle size
45–90 μm (mean 60 μm)
Capacity
12–30 µmol Cu2+/ml resin, 6x histidine-tagged proteins ≥15 mg/ml resin (dynamic binding capacity varies from protein to protein)
Maximum operating pressure
109 psi (7.5 bar)
Recommended flow rate
≤600 cm/hr at 25°C
Operating temperature range
4–40°C
Autoclavability (121°C, 30 min)
Yes (in 0.1 M sodium acetate)
Storage
4°C or ambient
Shelf life
≥1 year at ambient temperature in 20% ethanol