CFDA-SE #135-1201

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Use the CFDA-SE (5[6]Carboxyfluorescein diacetate, succinimidyl ester) cell-permeable dye to monitor cell population health.

CFDA-SE fluoresces after the acetate groups are cleaved by intracellular esterases creating CFSE. As the succinimidyl ester group reacts with primary amines of intracellular proteins, the CFSE is retained in the cell and is successively halved during cell divisions.

Key Benefits of the CFDA-SE Dye

  • Visualize up to 8 cell divisions
  • Smaller vial size to minimize contamination and reduce dilution steps
  • Convenient, easy-to-use format

Features of the CFDA-SE Dye

  • Fluorophore Properties
    • Excitation maximum: 492 nm
    • Emission maximum: 517 nm
    • Optimal excitation laser: 488 nm
    • Optimal S3™ 488/561 cell sorter filter set: FL1 525/30 nm
  • Fluorescent Spectrum

Fluorescent Spectrum

  • Molecular weight: 557.46 g/mol
  • Molecular formula: C29H19NO11
  • Chemical structure

Chemical Structure

  • Specifications
    • Store at –20°C
    • Protect from light

Related Products

In addition to CFDA, SE, we offer the CytoTrack™ Dyes listed below for detecting the fluorophore(s) of your choice:

Available Dyes Excitation Maximum, nm Emission Maximum, nm Optimal Excitation Laser, nm Optimal Filter Set, nm Catalog Number
CytoTrack Blue
403/454
403 454 405 449/38 135-1202
CytoTrack Green
511/525
511 525 488 FL1 525/30* 135-1203
CytoTrack Yellow
542/556
542 556 532 574/26 135-1204
CytoTrack Red
628/643
628 643 633 or 640 660/20 135-1205

* This optimal setting is recommended when using the 488/561 S3™ Cell Sorter System.

More Information

Learn more about the features and applications of the S3™ Cell Sorter and the ZOE™ Fluorescent Cell Imager.