Western Blotting Buffers and Reagents

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Overview

Colorimetric Detection of Proteins

Specific enzymatic detection of membrane-bound antigens.


  1. A membrane with unoccupied binding sites is incubated with primary antigen.
  2. Primary antibody to a specific antigen is incubated with the membrane.
  3. A blotting-grade antibody-enzyme conjugate is added to bind to the primary antibody.
  4. Color development reagent is added to the blot. The HRP or AP enzyme catalyzes the conversion of the substrate (S) to a colored precipitate (P) at the site of the antigen-antibody complex.

Western Blotting

Bio-Rad offers an extensive line of products for detection of proteins on western blots.

Blotting Membranes for Proteins

Bio-Rad's comprehensive line of blotting membranes are available in rolls or in sheets cut to fit the Mini-PROTEAN® Tetra blotting module. Membranes are also available in preassembled blotting membrane/filter paper sandwiches.

The 0.45 µm nitrocellulose membrane is most commonly used as a transfer medium for proteins. For transfer of low molecular weight proteins (<15,000), the 0.2 µm nitrocellulose membrane provides better sensitivity, especially with immunodetection assays.

Protein Blotting Buffers and Detection Kits

Get the essential reagents to perform colorimetric detection of proteins on western blots with the convenience of premixed buffers and enzyme substrate kits. Select from a combination of binding conjugates and color detection reagents, including alkaline phosphatase (AP)- or horseradish peroxidase (HRP)-conjugated secondary antibodies and HRP-conjugated protein A or protein G.

10x Phosphate Buffered Saline

161-0780EDU
1 L, buffer, 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, for use as western blotting wash solution; education use only

List Price:   $52.00
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10x Tris Buffered Saline

170-6435EDU
1 L, 10 x TBS for blotting applications; education use only

List Price:   $49.00
EDU Price:   $39.20   What is this?
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10x Tris/Glycine Buffer

161-0734EDU
1 L, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3; educational use only

List Price:   $35.00
EDU Price:   $28.00   What is this?
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10x Tris/Glycine Buffer

161-0771EDU
5 L cube, 10x premixed electrophoresis buffer, contains 25 mM Tris, 192 mM glycine, pH 8.3, for educational use only

List Price:   $112.00
EDU Price:   $89.60   What is this?
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1x Phosphate Buffered Saline with 1% Casein

161-0783EDU
1 L, blocking solution, for western blotting; education use only

List Price:   $112.00
EDU Price:   $89.60   What is this?
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1x Tris Buffered Saline with 1% Casein

161-0782EDU
1 L, blocking solution, for western blotting, 20 mM Tris, 500 mM NaCl, 1% (w/v) casein, pH 7.4; education use only

List Price:   $112.00
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10% Tween 20

161-0781EDU
1 L, diluted surfactant, for easy pipetting; education use only

List Price:   $43.00
EDU Price:   $34.40   What is this?
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Mini Trans-Blot Filter Paper

170-3932EDU
Pkg of 50, 7.5 x 10 cm, absorbent filter paper, for use with Mini-PROTEAN Tetra blotting module; education use only

List Price:   $57.00
EDU Price:   $45.60   What is this?
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Nitrocellulose Paper

162-0145EDU
Pkg of 10, precut nitrocellulose sheets, 0.45 μm, 7 x 8.4 cm; education use only

List Price:   $74.00
EDU Price:   $59.20   What is this?
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Nitrocellulose/Filter Paper Pack

166-2807EDU
0.45 μm, 7 x 8.4 cm, nitrocellulose membrane (8 sheets)/filter paper (16 sheets) sandwich; education use only

List Price:   $93.00
EDU Price:   $74.40   What is this?
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Nitrocellulose/Filter Paper Sandwiches

162-0214EDU
Pkg of 20, 7 x 8.5 cm, precut preassembled 0.45 μm blotting membrane/filter paper sandwich; fits Ready Gel/Mini-PROTEAN gels; education use only

List Price:   $156.00
EDU Price:   $124.80   What is this?
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Nitrocellulose/Filter Paper Sandwiches

162-0212EDU
Pkg of 20, 7 x 8.5 cm, precut preassembled 0.2 μm blotting membrane/filter paper sandwich; fits Ready Gel/Mini-PROTEAN gels; education use only

List Price:   $172.00
EDU Price:   $137.60   What is this?
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Number Description Options
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]
2317 Ready-to-Run Buffers and Solutions Brochure, Rev F Click to download [ Add to Cart (Free) ]