VMA/HVA/5-HIAA by HPLC

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VMA/HVA/5-HIAA by HPLC

Bio-Rad offers an improved application for VMA/HVA/5-HIAA by HPLC for use with manual or automated methods (on Gilson ASPEC™ systems). This assay permits either simultaneous, quantitative determination of vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), or the quantitative determination of vanillylmandelic acid (VMA) only in urine by HPLC for diagnosis of pheochromocytoma, neuroblastoma, and carcinoid syndrome. It features fast, easy specimen preparation with precise HPLC separation.

A manual method and an automated application are available. The automated application, which is designed for use with Gilson ASPEC™ XL or GX-271, offers high specimen through-put and reduced hands-on time, with an overnight processing option.

Test characteristics:

  • Principle: Isocratic HPLC method (Reversed Phase)
  • Duration of chromatography: 19 minutes (for VMA, HVA and 5-HIAA); 10 minutes (for VMA only)
  • Sample volume: 500 µL (manual method) / 50 µL (automated method) urine
  • Flow rate: 1.1 mL/min.
  • Electrochemical detection: 750 mV

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VMA/HVA/5-HIAA by HPLC Reagent Kit

195-4129
Reagents for simultaneous, quantitative determination of VMA, HVA, and 5-HIAA, or quantitative determination of VMA only, in urine by HPLC (100 tests)

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Analytical Column for VMA/HVA/5-HIAA by HPLC
Analytical Column for VMA/HVA/5-HIAA by HPLC

195-5961
One analytical column with 1 microguard cartridge for VMA, HVA and 5-HIAA by HPLC assay (500 injections)

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Micro-Guard Cartridge Holder

195-5030
1 holder for high performance liquid chromatography guard cartridge

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Capillary Connection Set
Capillary Connection Set

195-4088
Set of hardware to connect 2 HPLC cartridges (1 unit)

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Test Mix for VMA/HVA/5-HIAA by HPLC Reagent Kit

195-5969
Solution used to check the HPLC system performance (1 mL)

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Urine Calibrator Set (Mono-Level) for VMA/HVA/5-HIAA by HPLC Reagent Kit
Urine Calibrator Set (Mono-Level) for VMA/HVA/5-HIAA by HPLC Reagent Kit

195-5970
Urine-based standard containing vanillylmandelic (VMA), homovanillic (HVA), and 5-hydroxyindoleacetic acid (5-HIAA) for HPLC (2 x 5 mL)

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Mobile Phase for VMA/HVA/5-HIAA by HPLC Reagent Kit

195-6583
Mobile phase for VMA/HVA/5-HIAA by HPLC assay (1 x 1500 mL)

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Micro-Guard Refill Cartridges

195-6003
Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections

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Real-Time PCR Detection Systems for In Vitro Diagnostics (IVD)
Lyphochek Quantitative Urine Control, Level 1, Normal

376
Assayed, lyophilized, human urine based control for urine chemistry analytes, amines, and hormones; normal level (12 x 10 mL)

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Lyphochek™ Quantitative Urine Control, Level 2, Abnormal

377
Assayed, lyophilized, human urine based control for urine chemistry analytes, amines, and hormones; abnormal level (12 x 10 mL)

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