Bio-Rad’s Vitamin A/E by HPLC assay provides quantitative determination of Vitamin A (Retinol) and Vitamin E (Tocopherol) in serum by HPLC, for the diagnosis of vitamin deficiency. This assay allows for simultaneous determination of Vitamins A and E in a single run. Sample preparation eliminates interfering substances by precipitation, and a serum control set is available.
- Principle: Isocratic HPLC method (Reversed Phase)
- Duration of the chromatography: 7 min
- Sample volume: 200 µL serum or plasma
- Flow rate: 0.6 mL/min
- UV Detection: 340 nm (Vitamin A), 295 nm (Vitamin E)