VINEO™ Brettanomytest PCR Kit

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Overview

Key Benefits

VINEO™ Brettanomytest Key Benefits

  • A common DNA extraction protocol for "Brett" and "Zygo": the VINEO Extract DNA Kit
  • Specificity and sensitivity of real-time PCR
  • Result 2 hours after DNA extraction
  • Automatic and qualitative analysis: CFX Manager™ IDE
  • Same time with simultaneous PCR analysis of "Brett" and "Zygo"
  • Easy and ready-to-use kits
Protocol

VINEO™ Brettanomytest Protocol:

 

 

VINEO™ Brettanomytest - Literature Corner

Literature pieces available:

 

VINEO™ Brettanomytest Quick Guides Language
PF060 bio-rad
PF061 bio-rad
PF125 bio-rad
PF124 bio-rad

 

VINEO™ Brettanomytest Flyers Language
PF16425 bio-rad
PF288 bio-rad

 

Background

During the wine manufacturing process, there are two steps of fermentation, alcoholic fermentation then malolactic fermentation. During alcoholic fermentation, yeast transforms sugar into alcohol. Before fermentation, Saccharomyces is present in the grape juice, as well as other yeasts such as Brettanomyces bruxellensis, commonly known as Brett. After alcoholic fermentation, B. bruxellensis can still be present in the matrix.

The presence of B. bruxellensis is an important issue, as it has been shown that its growth is linked to the concentration of 4-ethylphenol, which imparts a strong off-taste, leading to consumer rejection of the wine. To avoid wine spoilage by B. bruxellensis, some preventative actions can be taken such as racking, clarification, and sulfiting. However, if Brett spoilage has already begun, the winemaker must undertake additional remedial steps such as filtration or heat treatment, increasing production cost. If the concentration of ethylphenol is too high, the lot may have to be destroyed.

Rapid results

Bio-Rad has developed a new tool for the sensitive, selective, and rapid quantification of B. bruxellensis. The VINEO Brettanomytest Kit monitors Brettanomyces risk. The VINEO method is based on real-time PCR technology and entails two simple steps: DNA extraction followed by detection and quantification of the yeast. Current microbiology culture methods can take 7–14 days before results are available, whereas results are available the same day of analysis with the VINEO Kit. The included software automatically provides an easy interpretation of the results.

VINEO™ Extract DNA Kit

354-8100
96 tests

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VINEO™ Brettanomytest PCR Kit

354-8101
96 tests

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MiniOpticon™ real-time PCR detection system

359-1592
including optical housing, MJ Mini thermal cycler, analysis software, 1 unit

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CFX96 Real-Time PCR Detection System

360-0037
Modular thermal cycler platform, includes C1000 thermal cycler chassis and CFX96 optical reaction module.

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Number Description Options
FSD_881111 Instruction Manual, VINEO™ Extract DNA Kit (English/Français/Español/Italiano) Click to download
FSD_881112 Instruction Manual, VINEO™ Brettanomytest PCR Kit (English/Français/Español/Italiano) Click to download
PF_288_en VINEO™ Brettanomytest PCR Kit Brochure, Rev A Click to download
PF_236 Brettanomyces, or Management of a Contaminant Micro-organism, Rev A Click to download
FSD_PF061 VINEO™ Extract DNA Kit Quick Guide, Rev C Click to download
PF_173 CFX Manager™ Software, Industrial Diagnostic Edition, Quick Guide, Rev B Click to download