UNOsphere™ S Media

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Overview

Specifications
Type of ion exchanger Strong cation
Functional group -SO3-
Total ionic capacity 280 ± 30 µeq/ml
Dynamic binding capacity*
150 cm/hr 60 mg IgG/ml
1,200 cm/hr 30 mg IgG/ml
Median particle size 80 µm
Recommended linear flow rate range** 50–1,200 cm/hr
Chemical stability
1.0 M NaOH (20°C) Up to 2,000 hr
1.0 M HCl (20°C) Up to 200 hr
pH stability range pH 1–14
Regeneration conditions 1–2 M NaCl
Storage conditions 20% Ethanol
0.1 N NaOH for 30 days

 * 10% breakthrough capacity determined with 4.5 mg/ml human IgG in a 1.1 x 10 cm column.
** UNOsphere S support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.

Binding and Backpressure Properties

Binding and backpressure properties of UNOsphere S support. Column size, 1.1 x 20 cm; sample, 2 mg/ml human IgG; buffer, 50 mM sodium acetate, pH 5.0 (), backpressure; (), 10% breakthrough capacity.

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Purification of IgG

Purification of murine IgG1 on UNOsphere S column. Column size, 0.5 x 10 cm (2 ml); sample, 15 ml (6.6 mg) of murine IgG1-conditioned medium. The sample was loaded onto the column in 20 mM citrate-phosphate buffer, pH 4.0, washed, and eluted in a linear gradient of 0–100% 20 mM citrate-phosphate, pH 8.0 in 10 column volumes at a flow rate of 2.0 ml/min (600 cm/hr). Each fraction was 2.0 ml. (), A280; (), buffer pH.


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UNOsphere Media Purification Analysis
SDS-PAGE gel of UNOsphere-purified murine IgG1

SDS-PAGE gel of UNOsphere-purified murine IgG1. Fractions from the chromatography run shown were separated on a 4–20% precast gel. Lane 1, conditioned medium; lane 2, flowthrough; lanes 3–8, fractions 25, 26, 27, 28, 29, and 30; lane 9, protein A–purified murine IgG1 from culture medium.

UNOsphere S cation exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media. UNOsphere, a hydrophilic polymeric support based on a single-step polymerization process, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

UNOsphere S support is a strong cation exchanger.

Features include:

  • Optimized to operate under 2 bar backpressure at linear velocities of 1,200 cm/hr
  • Stable to 1 M NaOH exposure up to 10,000 hours
  • Efficient capture of biomolecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads
  • Robust polymer designed to withstand repeated clean-in-place cycles
  • Biopharmaceutical manufacturing quantities available
  • Fully supported for regulatory submission

Most production chromatography systems have maximum pressure limits of 3 bar. The median particle size of UNOsphere S media is 80 µm, which generates a backpressure of less than 2 bar at 1,200 cm/hr. The highly macroporous nature of UNOsphere S media provides high binding capacities of 60 mg human IgG per ml of media at 150 ml/min. Harsh clean-in-place and sanitization cycles will not affect the long-term stability of UNOsphere media.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Type of ion exchanger Strong cation
Functional group -SO3-
Total ionic capacity 280 ± 30 µeq/ml
Dynamic binding capacity*  
150 cm/hr 60 mg IgG/ml
1,200 cm/hr 30 mg IgG/ml
Median particle size 80 µm
Recommended linear flow rate range** 50–1,200 cm/hr
Chemical stability  
1.0 M NaOH (20°C) Up to 2,000 hr
1.0 M HCl (20°C) Up to 200 hr
pH stability range pH 1–14
Regeneration conditions 1–2 M NaCl
Storage conditions 20% Ethanol
0.1 N NaOH for 30 days
* 10% breakthrough capacity determined with 4.5 mg/ml human IgG in a 1.1 x 10 cm column.  
** UNOsphere S support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.  
UNOsphere S Support #156-0113
UNOsphere S Support

156-0111
25 ml, strong cation exchange chromatography media, 120 μm particle size

List Price:   $113.00
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UNOsphere S Support #156-0115
UNOsphere S Support

156-0113
100 ml, strong cation exchange chromatography media, 120 μm particle size

List Price:   $340.00
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UNOsphere S Support #156-0117
UNOsphere S Support

156-0115
500 ml, strong cation exchange chromatography media, 120 μm particle size

List Price:   $1,168.00
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UNOsphere S Support

156-0117
10 L, strong cation exchange chromatography media, 80 μm particle size

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Foresight™ UNOsphere™ S Plates

732-4710
2 x 96-well plates, 20 μl, filter plate prepacked with UNOsphere S strong cation exchange chromatography media

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Foresight™ UNOsphere™ S RoboColumn Unit

732-4813
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with UNOsphere S strong cation exchange chromatography media

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Foresight™ UNOsphere™ S RoboColumn Unit

732-4814
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with UNOsphere S strong cation exchange chromatography media

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Foresight™ UNOsphere™ S Column

732-4730
Pkg of 1, 1 ml, column prepacked with UNOsphere S strong cation exchange chromatography media

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Foresight™ UNOsphere™ S Column

732-4750
Pkg of 1, 5 ml, column prepacked with UNOsphere S strong cation exchange chromatography media

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Number Description Options
2669 UNOsphere S Cation Exchange Media Product Information Sheet, Rev C Click to download [ Add to Cart (Free) ]
5515 Guidelines for Using UNOsphere S Cation Exchange Support, Rev A Click to download
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
10005454 Instruction Manual, Bio-Scale Mini UNOsphere Q & S Cartridges, 1 and 5 ml, Rev C Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download