UNOsphere™ Q Media

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Overview

Binding and Backpressure Properties

Binding and backpressure properties of UNOsphere Q support: A 5 mg/ml sample of BSA in 10 mMTris, pH8.5, was loaded onto a 1.1 x 20 cm column. Red, backpressure; blue, 10% breakthrough capacity.

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Purification and Analysis of Plasmid DNA
Purification of plasmid DNA on a UNOsphere Q column

Purification of plasmid DNA on a UNOsphere Q column. Clarified bacterial lysate (10 ml, adjusted to pH 8.0) was loaded onto a 0.5 x 11 cm column (2.1 ml) in buffer A (10 mM sodium phosphate, 0.3 M NaCl, 1 mM EDTA, pH 8.0). The sample was eluted with a 0–40% gradient of buffer B (10 mM sodium phosphate, 1.0 M NaCl, 1 mM EDTA, pH 8.0) at a flow rate of 2 ml/min (600 cm/hr). The column was washed with 10 column volumes of 40% buffer B, followed by 100% buffer B for 5 column volumes. The effluent was monitored at 254 nm. Each fraction was 5 ml. Blue, A254; red, conductivity; black, theoretical gradient.

Analysis of plasmid DNA purified on UNOsphere Q support

Analysis of plasmid DNA purified on UNOsphere Q support. Fractions were separated on a 0.8% agarose gel. Lane 1, 1 kb marker (catalog #170-8204); lane 2, crude lysate; lane 3, flowthrough (fractions 2–6); lane 4, fractions 9–18; lane 5, fractions 19–30; lane 6, fractions 31–35; lane 7, fraction 36.

Restriction enzyme analysis of plasmid DNA purified on UNOsphere Q support. Lane 1, 1 kb marker; lane 2, undigested clarified lysate; lane 3, EcoRI-digested clarified lysate; lane 4, undigested fractions 31–35; lane 5, digested fractions 31–35; lane 6, undigested fraction 36; lane 7, digested fraction 36.

PDF Bulletin 2724 To read more click here

UNOsphere ion exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media. UNOsphere, a hydrophilic polymeric support based on a single-step polymerization process, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility. UNOsphere Q media are strong anion exchangers.

Features include:

  • Optimized to operate under 2 bar backpressure at linear velocities of 1,200 cm/hr
  • Stable to 1 M NaOH exposure up to 10,000 hours
  • Efficient capture of biomolecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads
  • Robust polymer designed to withstand repeated clean-in-place cycles
  • Biopharmaceutical manufacturing quantities available
  • Fully supported for regulatory submission

Most production chromatography systems have maximum pressure limits of 3 bar. The median particle size of UNOsphere Q support is 120 µm, which generates a backpressure of less than 2 bar at 1,200 cm/hr. The highly macroporous nature of UNOsphere Q support provides high binding capacities that range from 180 to 125 mg BSA per ml in the linear velocity range of 150–1,200 cm/hr, respectively. Harsh clean-in-place and sanitization cycles will not affect the long-term stability or performance of UNOsphere Q media.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Type of ion exchanger Strong anion
Functional group -N(Ch3)3+
Total ionic capacity 120 µeq/m
Dynamic binding capacity*  
     at 150 cm/hr 180 mg BSA/ml
     at 600 cm/hr 125 mg BSA/ml
Median particle size 120 µm
Recommended linear flow rate range** 50–1,200 cm/hr
Chemical stability  
     in 1.0 M NaOH (20°C) Up to 10,000 hr
     in 1.0 M HCl (20°C) Up to 200 hr
pH stability range pH 1–14
Regeneration conditions 1–2 M NaCl
Storage conditions 20% ethanol
0.1 M NaOH for 30 days
* 10% breakthrough capacity determined with 2.0 mg/ml BSA in a 1.1 x 10 cm column.  
** UNosphere Q support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.  
UNOsphere Q Support

156-0101
25 ml, strong anion exchange chromatography media, 120 μm particle size

List Price:   $113.00
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UNOsphere Q Support

156-0103
100 ml, strong anion exchange chromatography media, 120 μm particle size

List Price:   $340.00
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UNOsphere Q Support

156-0105
500 ml, strong anion exchange chromatography media, 120 μm particle size

List Price:   $1,168.00
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UNOsphere Q Support

156-0107
10 L, strong anion exchange chromatography media, 120 μm particle size

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Foresight™ UNOsphere™ Q Plates

732-4714
2 x 96-well plates, 20 μl, filter plate prepacked with UNOsphere Q strong anion exchange chromatography media

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Foresight™ UNOsphere™ Q RoboColumn Unit

732-4819
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with UNOsphere Q strong anion exchange chromatography media

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Foresight™ UNOsphere™ Q Column

732-4732
Pkg of 1, 1 ml, column prepacked with UNOsphere Q strong anion exchange chromatography media

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Foresight™ UNOsphere™ Q Column

732-4752
Pkg of 1, 5 ml, column prepacked with UNOsphere Q strong anion exchange chromatography media

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Number Description Options
2724 UNOsphere Q Anion Exchange Support Product Information Sheet, Rev D Click to download [ Add to Cart (Free) ]
2729 UNOsphere Q Support Technical Data, Rev A Click to download [ Add to Cart (Free) ]
5735 Purification of a Monoclonal Antibody Using a Combination of UNOsphere Rapid S and UNOsphere Q Media Ion Exchange Chromatography After Protein A Capture, Rev A Click to download [ Add to Cart (Free) ]
5543 Effective Cleaning and Sanitizing of Anion Exchange Chromatography Resins, Rev A Click to download
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
10005454 Instruction Manual, Bio-Scale Mini UNOsphere Q & S Cartridges, 1 and 5 ml, Rev C Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download