UNOsphere Anion Exchange Resin

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UNOsphere Anion Exchange Resin - Anion Exchange Resin

Overview

Specifications
  UNOsphere Q UNOsphere S
Type of ion exchanger Strong anion Strong cation
Functional group -N+(CH3)3 -SO3-
Dynamic binding capacity*    
150 cm/hr 180 mg BSA/ml 60 mg IgG/ml
600 cm/hr 125 mg BSA/ml 30 mg IgG/ml
Shipping counterion Cl- Na+
Median particle size 120 µm 80µm
Recommended linear flow rate range** 50–1,200 cm/hr 50–1,200 cm/hr
Chemical stability    
1.0 M NaOH (20°C) Up to 10,000 hr Up to 10,000 hr
1.0 M HCl (20°C) Up to 200 hr Up to 200 hr
Volume changes**    
pH 4 – 10 <5% <5%
0.01 – 1.0 M NaCl <5% <5%
pH stability 1 – 14 1 – 14
Autoclavability Yes Yes
Antimicrobial agent 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol or 0,1 M NaOH 20% ethanol or 0,1 M NaOH

* 10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere S) and 2.0 mg/ml BSA (UNOsphere Q) in a 1.1 x 20 cm column.
** UNOshpere packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.

Product Selection by Technique
   
Scale
Packaging Format
Characteristics Product Type Analytical Pilot/Preparative Process Prepacked Bottled
Ion Exchange Chromatography
Separation by charge UNOsphere™ media     UNOsphere Q, S, Rapid S
General biomolecules purification Macro-Prep® media     Macro-Prep High Q, DEAE, High S, CM
Applicable to all antibody purifications Macro-Prep 25 media     Macro-Prep 25 Q, 25 S
UNO® columns   UNO Q, S  
Bio-Scale™ Mini Cartridges     UNOsphere Q, S  
High capacity         Macro-Prep High Q, S  
Good capture technique AG® resins   AG
Chelex® resins Chelex
Bio-Rex® resins Bio-Rex
Hydroxyapatite and Fluoroapatite Chromatography
Unique selectivity CHT™ 80 µm support     CHT, Types I and II
Alternative to protein A for antibody purification CHT 40 µm support     CHT, Types I and II
CHT 20 µm support   CHT, Types I and II
CFT 40 µm support CFT, Types I and II
Removal of leached protein A, aggregates, endotoxins, and DNA Bio-Gel® HT and HTP media     Bio-Gel HT, HTP
Bio-Scale columns   CHT Type I (10 µm)  
CHT Type II (20 µm)  
Affinity Chromatography
Purification of His- and GST- tagged proteins Profinity™ IMAC and GST media   Bio-Scale Mini Profinity IMAC
Separation by biological interaction Affi-Gel resins     Affi-Gel® Protein A*
Affi-Gel Blue*
DEAE Affi-Gel Blue*
CM Affi-Gel Blue*
High selectivity           Affi-Gel boronate, heparin**
High purity in a single step           Affi-Gel Hz, 10, 15**
Affi-Gel 102**
Excellent capture technique Profinity epoxide media     Profinity epoxide**
Affi-Prep media   Affi-Prep protein A*
Affi-Prep polymyxin*
Bio-Scale Mini cartridges   Profinity IMAC and GST  
Econo-Pac® columns   Affi-Gel Blue  
DEAE Affi-Gel Blue
Affi-Gel protein A
Size Exclusion Chromatography
Separation by size Bio-Gel P media     Bio-Gel P
MW determination Bio-Beads™ S-X media     Bio-Beads S-X
Applicable to all antibody purifications Bio-Sil® and Bio-Silect™ columns     Bio-Sil, Bio-Silect  
Desalting or buffer exchange Bio-Scale Mini cartridges     Bio-Gel P-6  
High resolution Econo-Pac columns     Econo-Pac 10DG  
Hydrophobic Interaction Chromatography
Separation by hydrophobicity Macro-Prep HIC media     Macro-Prep t-butly
Macro-Prep methyl

* Ready to use.
** Activated.

UNOsphere Q support
Binding and backpressure properties of UNOsphere mediaBinding and backpressure properties of UNOsphere media. Column size, 1.1 x 20 cm. UNOsphere Q media loaded with 5mg/ml BSA in 10 mM Tris, pH 8.5. Backpressure (orange); 10% breakthrough capacity (blue).
Plasmid DNA

Plasmid DNA separation using UNOsphere media.
Analysis of DNA

Analysis of fractions generated from DNA purification using UNOsphere media.

UNOsphere Q support is used for strong anion exchange chromatography. UNOsphere ion exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media. UNOsphere, a hydrophilic polymeric support, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

Key Features

  • Efficient capture of biopharmaceutical molecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads engineered for high mechanical stability and low backpressures
  • Robust polymer designed to withstand repetitive clean-in-place cycles
  • Optimized to operate under 2 bar backpressure at velocities of 1,200 cm/hr
  • Stable to 1 M NaOH exposure to 10,000 hr
  • Availability in biopharmaceutical quantities
  • Fully supported for regulatory submissions

 

UNOsphere Q Support

156-0101
25 ml, strong anion exchange chromatography media, 120 μm particle size

List Price:   $113.00
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UNOsphere Q Support

156-0103
100 ml, strong anion exchange chromatography media, 120 μm particle size

List Price:   $340.00
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UNOsphere Q Support

156-0105
500 ml, strong anion exchange chromatography media, 120 μm particle size

List Price:   $1,168.00
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UNOsphere Q Support

156-0107
10 L, strong anion exchange chromatography media, 120 μm particle size

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Number Description Options
2729 UNOsphere Q Support Technical Data, Rev A Click to download [ Add to Cart (Free) ]
2724 UNOsphere Q Anion Exchange Support Product Information Sheet, Rev D Click to download [ Add to Cart (Free) ]
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download