TransFectin™ Lipid Reagent

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TransFectin reagent is a cationic lipid reagent that delivers the highest efficiencies and expression levels over a broad range of cell lines, including some difficult-to-transfect and primary cells, while minimizing damaging cytotoxic effects observed with other high-efficiency general transfection reagents. It provides high transfection efficiencies with lower amounts of lipid than most other lipid reagents, allowing more experiments per vial of reagent and lower costs per transfection.

TransFectin lipid reagent is a mixture of a proprietary cationic compound and a co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine). These compounds have been optimized for intracellular delivery of nucleic acids into cultured mammalian cells in the presence of serum at cell densities from 40 to >90%. For most cell lines, high levels of expression can be obtained using concentrations of TransFectin reagent and DNA suggested for given sizes of plates/wells.

Key Features

  • Effectiveness — yields high-efficiency transfections and transgene activity for a broad range of cells
  • Low cytotoxicity — helps maintain cell viability
  • Flexibility — excellent performance at culture densities between 40 and 90% and in the presence or absence of serum-containing medium
  • Simplicity — 3-step protocol and no posttransfection media changes required for most cell types

Other Lipid Reagents

Bio-Rad offers a family of lipid transfection reagents, each of which is optimal for certain applications. TransFectin lipid reagent is a general-purpose lipid that is ideal for plasmid delivery. siLentFect™ lipid reagent is an RNAi-specific lipid that is ideal for siRNA delivery. It can also be used to perform cotransfection with a small reporter-plasmid along with the siRNA molecule.

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TransFectin™ Lipid Reagent

1703350
0.5 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery

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$226.00
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TransFectin™ Lipid Reagent

1703351
1.0 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery

List Price:
 
$421.00
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TransFectin™ Lipid Reagent

1703352
5 x 1.0 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery

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$1,549.00
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Number Description Options
2873 TransFectin Lipid Reagent Brochure, Rev A Click to download
2874 TransFectin Lipid Reagent Flier, Rev B Click to download
4106254 Instruction Manual, TransFectin Lipid Reagent, Rev A Click to download
5343 Transfection Reagent References, Rev C Click to download
3197 Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types, Rev A Click to download
3138_015 TransFectin Lipid Reagent Protocol, Human, A459, Lung Carcinoma Click to download
3138_005 TransFectin Lipid Reagent Protocol, Rat, PC12, Pheochromocytoma Click to download
3138_009 TransFectin Lipid Reagent Protocol, Human, 143B, Bone Marrow Osteosarcoma Click to download
5226 Highly Efficient Transfection of Mouse ES Cells With TransFectin Lipid Reagent Click to download
3138_018 TransFectin Lipid Reagent Protocol, Human, HEK 293, Kidney Click to download
3138_017 TransFectin Lipid Reagent Protocol, Human, HEK 293T, Kidney Click to download