Supported Nitrocellulose Membrane, 0.2 µm

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Overview

Available Formats

Blotting membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Supported Nitrocellulose Membranes

Ideal for:

  • Protein blotting
  • DNA/RNA blotting
  • Dot/slot blot screening

 

Bio-Rad's 0.2 µm pore–size nitrocellulose membrane is dense 100% nitrocellulose. Pure nitrocellulose is the most frequently used membrane for the transfer of both proteins and nucleic acids. It is a proven medium for western, Northern, and Southern blotting that produces excellent signal-to-noise results. Bio-Rad nitrocellulose is easy to handle, wets readily with water-based solutions, and resists the brittleness usually associated with nitrocellulose. The 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins: the smaller pore size minimizes protein sample loss due to transfer through the membrane.

Features and Benefits

  • Compatible with all standard immunoblotting and nucleic acid detection methods
  • Provides superior binding capacity without background interference
  • Smaller pore size minimizes low molecular–weight protein sample loss
  • Available in three formats:
    • Convenient precut 0.2 µm membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transfer of low molecular–weight proteins and nucleic acids <500 bp
  • Protein transfer from SDS-PAGE gels to membrane in preparation for western blotting
  • Transfer of nucleic acids in preparation for Northern or Southern blotting
  • Immunodetection of target protein and probe hybridization of nucleic acids

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

Supported Nitrocellulose Membrane

162-0097
Pkg of 1 roll, 0.2 μm, 30 cm x 3 m, bulk blotting membrane for proteins and nucleic acids

List Price:   $399.00
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Supported Nitrocellulose Membranes

162-0096
Pkg of 10, 0.2 μm, 15 x 15 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $157.00
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Supported Nitrocellulose Membranes

162-0095
Pkg of 10, 0.2 μm, 7 x 8.4 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $77.00
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Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]