Precision Melt Supermix

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Overview

Robust HRM for Class I–IV SNPs
Robust HRM

Precision melt supermix delivers robust HRM for class I–IV SNPs. Discrimination of A, class I (A to G substitution); B, class II (G to T substitution); C, class III (G to C substitution); and D, class IV (A to T substitution) SNP genotypes from mouse genomic DNA using precision melt supermix. Wild type (), heterozygote (), and homozygous mutant () are shown in the difference plots normalized to wild-type samples. HRM analysis was performed on a CFX384™ real-time PCR detection system and genotypes were automatically assigned by Precision Melt Analysis™ software. Amplification was carried out for 35 cycles. Total run time including melt curve = 150 min. RFU, relative fluorescence units.

Accurate Methylation Detection
Accurate Methylation Detection

Accurate methylation detection with precision melt supermix. A, normalized melt curve; B, difference curve. Mixtures of methylated and unmethylated human genomic DNA of varying ratios were analyzed using HRM on a CFX384™ real-time PCR detection system. Increasing amounts of methylated DNA (, 0%; , 2%; , 5%; , 50%; , 75%; , 95%; and , 100%) were analyzed for methylation of the human RARB2 gene. The genomic region contains 7 CpG sites and is 88 base pairs in length. Total run time including melt curve = 190 min. RFU, relative fluorescence units.

Exceptional Stability
Exceptional Stability

Exceptional stability enables high-throughput genotyping analysis with precision melt supermix. Specific amplification and accurate discrimination of a class IV SNP (84 bp amplicon) from mouse genomic DNA was performed on a CFX384™ real-time PCR detection system either 0 hr (A) or 48 hr (B) after reaction setup. Wild type (), heterozygote (), and homozgous mutant () are shown in the difference plots normalized to wild-type samples. Total run time including melt curve = 150 min. RFU, relative fluorescence units.

The precision melt supermix is a superior high-resolution melt (HRM) supermix delivering optimal discrimination for numerous applications including:

  • Genotyping
  • Mutation analyses
  • Methylation studies
  • Species identification
  • Epigenetic analyses
  • De novo SNP discovery

Key Features, Benefits and Applications of the Precision Melt Supermix

  • Sensitive and specific discrimination of class I–IV single nucleotide polymorphisms (SNPs) across a broad range of amplicons
  • Ideal solution for insertion/deletions >6 base pairs
  • Accurate detection of the percent of CpG methylation status for epigenetic studies
  • Ideal for mutation screening of small mutations or using a primer walking approach for larger regions
  • Ideal cost-saving application for upstream and downstream next generation sequencing pre-screens and post-validations
  • Exceptional room temperature stability for high-throughput HRM studies
  • Optimized formulation containing EvaGreen dye delivers robust PCR and HRM performance

Available Formats

Precision melt supermix is available in formats of 2 ml (200 x 20 µl reactions, 172-5110) or 10 ml (1,000 x 20 µl reactions, 172-5112).

Instrument Compatibility

For use with all HRM-ready real-time PCR systems; dye calibration may be required.

Related Products

Bio-Rad offers a complete workflow solution for high resolution melt analysis, including:

  • Precision melt analysis software with calibration kit (184-5025)
  • Precision melt analysis software (184-5015)
  • Melt calibration kit (184-5020)
Storage at –20°C  Guaranteed for 6 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Precision Melt Supermix

172-5110
2 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 200 x 20 µl reactions

List Price:   $163.00
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Precision Melt Supermix

172-5112
10 x 1 ml, 2x real-time PCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, EvaGreen dye, stabilizers, for 1,000 x 20 µl reactions

List Price:   $683.00
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Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
6137 Precision Melt Supermix Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
10022094 Instruction Manual, Precision Melt Supermix, Rev B Click to download