SmartSpec Plus Spectrophotometer



Nucleic Acid Quantitation
Wavelength scan of a DNA sample from 225 to 350 nm.

Wavelength scan of a DNA sample from 225 to 350 nm. The absorbance maximum is at 261 nm. The instrument can be programmed to automatically subtract the background absorbance.

Protein Quantitation Curve
Protein Quantitation Curve

Standard curve generated on the SmartSpec Plus spectrophotometer using the Lowry protein assay.

Cuvette Selection Guide
Min. Volume Max. Volume Cuvette Type Pathlength Catalog Number
1,000 µl 3,500 µl Standard cuvette, quartz 10 mm 1702502
500 µl 1,400 µl Semimicrovolume cuvette, quartz 10 mm 1702503
200 µl 700 µl Microvolume cuvette, quartz 10 mm 1702504
80 µl 100 µl Submicrovolume cuvette, quartz 10 mm 1702505
50 µl 1,500 µl TrUView cuvette 10 mm 1702510
Hellma TrayCell with SmartSpec Plus

Schematic representation of the Hellma TrayCell device. A, loading the device; B, device in the SmartSpec Plus spectrophotometer.

PDF Bulletin 5693 To read more click here

The SmartSpec™ Plus spectrophotometer has a wider range of features and functions than many other benchtop spectrophotometers, offering performance, stability, and functionality at an affordable price.

The UV/visible SmartSpec Plus spectrophotomer has a working wavelength range of 200–800 nm. It is the perfect tool for routine applications such as:

  • Quantitation of DNA, RNA, and oligonucleotides
  • Quantitation of proteins via the Bradford, Lowry, and BCA assay methods
  • Monitoring bacterial culture growth
  • Simple kinetic assays
  • Wavelength scans with peak detection

A simple, menu-driven interface simplifies assays and provides answers to common sample computations at the touch of a button. Conversion factors can be stored and modified. The SmartSpec Plus spectrophotometer is capable of performing calculations and providing results that include:

  • A260/A280 ratio for nucleic acid purity
  • Quantitation that takes dilution factors into account
  • Sample concentration in µg/ml (additionally in pmol/µl for oligonucleotides)
  • Molar extinction coefficient and molecular weight of oligonucleotides

At the end of an assay, a report can be printed that shows the user's identification, date, and results.

Nucleic Acid Quantitation

The SmartSpec Plus spectrophotometer offers a complete solution for the quantitation of PCR products, nucleic acid preparations, or samples to be used in bacterial transfection protocols. dsDNA, ssDNA, or RNA can be quantitated by selecting from preprogrammed conversion factors or by entering a value that is best for the sample being assayed. The instrument provides absorbance values, concentration, and purity values so you can proceed confidently with your downstream experiments.

The SmartSpec Plus spectrophotometer also simplifies the quantitation of DNA and RNA oligonucleotides. When you enter the sequence, length, or composition, the spectrophotometer displays sample concentration in µg/ml and pmol/µl and automatically performs calculations of molar extinction coefficients and molecular weights.

Protein Quantitation

The SmartSpec Plus spectrophotometer has preprogrammed methods for quantitating proteins by the Bradford, Lowry, and BCA methods. Features built into each assay method facilitate data collection and present a complete analysis of assay results. The easy-to-follow menu guides you through the entire assay, ensuring that all standards and replicates are read.

  • Standards can be analyzed in groups of up to 9 replicates
  • Up to 10 standard curves can be stored under user-assigned names
  • Mean and standard deviation values are automatically calculated for each replicate group
  • Printable report includes a standard curve with r2 value

Additional Features

The SmartSpec Plus spectrophotometer also features:

  • Built-in printer
  • A Xenon flash lamp that extends lamp life and reduces maintenance costs
  • A user interface with a choice of 6 languages: English, French, German, Italian, Japanese, or Spanish
  • Spectral scans from 200 to 800 nm
  • Easy-to-use, menu-driven operation
  • Compact, space-saving design


Light souce Xenon lamp
Detector Photodiode array
Display 2 x 24 alphanumeric backlit LCD
Printer Built-in alphanumeric/graphic printer
Interface RS–232 serial port
Wavelength range 200–800 nm
Wavelength accuracy (at 18–27°C) <±1.0 nm from 200 to 250, 300 to 800 nm
  <±0.5 nm from 250 to 300 nm
Spectral bandwidth <5 nm
Absorption accuracy ±0.01 at 0.5 AU, ±0.02 at 1.0 AU
Absorption repeatability ±0.005 at 0.5 AU
Stray light <0.2% at 230 nm using Nal standard
Operating temperature 15–35°C
Beam height (z-dimension) 8.5 mm
Power requirement 90–260 VAC, 47–63 Hz
Regulatory CE, TUV
Dimensions 33 x 26 x 14 cm (W x D x H)
Weight ~ 3.8 kg

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SmartSpec Plus Spectrophotometer

90-260 V, UV/visible benchtop spectrophotometer for nucleic acid and protein quantitation, wavelength range 200–800 nm, built-in thermal printer

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Number Description Options
4006221 Instruction Manual, SmartSpec™ Plus Spectrophotometer, Rev A Click to download
4006168 Instruction Manual, SmartSpec 3000 Spectrophotometer, Rev D Click to download
2827 SmartSpec Plus Spectrophotometer Flier, Rev A. Click to download
2826 SmartSpec Plus Spectrophotometer Brochure, Rev B Click to download [ Add to Cart (Free) ]
5693 Measurement of low Volume DNA Samples Using the Hellma TrayCell With the SmartSpec Plus Spectrophotometer, Rev A. Click to download