Silver Stain Plus Kit

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Silver staining is a highly sensitive method for detecting proteins and nucleic acids in polyacrylamide slab gels. Bio-Rad silver stain kits are 10–50-fold more sensitive than Coomassie Brilliant Blue R-250 for proteins (detection is ~0.1 ng/mm2) and 2–5-fold more sensitive than ethidium bromide for single and double-stranded DNA and RNA.

Silver Stain Plus™ stain is the most sensitive and easiest to use silver stain. The Silver Stain Plus kit is derived from the method developed by Gottlieb and Chavko (1987). The chemistry has been modified for both protein and nucleic acid staining in polyacrylamide and agarose gels. The Silver Stain Plus kit can detect as little as 0.6 ng of protein and DNA and requires only three steps, allowing visualization of your sample in less than 1 hour.

Reference

Gottlieb M and Chavko M (1987). Silver staining of native and denatured eukaryotic DNA in agarose gels. Anal Biochem 165, 33-37.

Silver Stain Plus Kit

161-0449
Kit for detection of nanogram quatities of DNA and protein in polyacrylamide and agarose gels, for 13 full-size or 40 mini gels

List Price:   $263.00
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Development Accelerator Reagent

161-0448
50 g, replacement component of the Silver Stain Plus kit (#161-0449)

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Fixative Enhancer Concentrate

161-0461
1 L, replacement component of the Silver Stain Plus kit (#161-0449)

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Silver Complex Solution

161-0462
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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Reduction Moderator Solution

161-0463
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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Image Development Reagent

161-0464
100 ml, replacement component of the Silver Stain Plus kit (#161-0449)

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Number Description Options
LIT442 Instruction Manual, Silver Stain Plus, Rev E Click to download