Sequi-Blot PVDF Membrane

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Overview

Available Formats

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

PVDF Membranes

Ideal for:

  • Protein blotting
    (Immun-Blot® PVDF membrane)
  • Protein sequencing
    (Sequi-Blot™ PVDF membrane)

 

Comparison of Membranes

Superior retention of proteins by Sequi-Blot™ PVDF membrane.

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

Sequi-Blot™ PVDF membrane is ideal for protein sequencing: it retains target protein very strongly during protein transfer and provides the binding capacity to sequence even low-abundance samples.

Features and Benefits

  • Mechanical strength and chemical resistance provide ideal substrate for solvents involved in protein sequencing
  • Available in three formats:
    • Convenient precut membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Protein transfer from SDS-PAGE gels to membrane
  • Protein sequencing of target protein

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

Sequi-Blot PVDF Membrane #162-0184
Sequi-Blot PVDF Membrane

162-0184
Pkg of 1 roll, 0.2 μm, 26 cm x 3.3 m, bulk membrane for protein sequencing; binding capacity 170–200 μg/cm2

List Price:   $422.00
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Sequi-Blot PVDF Membrane #162-0182
Sequi-Blot PVDF Membrane

162-0182
Pkg of 10, 0.2 μm, 20 x 20 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

List Price:   $286.00
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Sequi-Blot PVDF Membrane #162-0181
Sequi-Blot PVDF Membrane

162-0181
Pkg of 10, 0.2 μm, 15 x 15 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

List Price:   $174.00
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Sequi-Blot PVDF Membrane #162-0180
Sequi-Blot PVDF Membrane

162-0180
Pkg of 10, 0.2 μm, 10 x 15 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

List Price:   $149.00
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Sequi-Blot PVDF Membrane #162-0186
Sequi-Blot PVDF Membrane

162-0186
Pkg of 10, 0.2 μm, 7 x 8.4 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

List Price:   $112.00
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Sequi-Blot PVDF Membrane #162-0256
(Discontinued) Sequi-Blot PVDF Membrane

162-0256
Pkg of 10, 0.2 μm, 25 x 28 cm, precut membrane for protein sequencing; binding capacity 170–200 μg/cm2

This product is no longer available. Please contact customer service for more information on replacement product.
Sequi-Blot PVDF/Filter Paper Sandwiches #162-0236
Sequi-Blot PVDF/Filter Paper Sandwiches

162-0236
Pkg of 20, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Criterion and Criterion XT gels

List Price:   $390.00
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Sequi-Blot PVDF/Filter Paper Sandwiches #162-0237
Sequi-Blot PVDF/Filter Paper Sandwiches

162-0237
Pkg of 50, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Criterion and Criterion XT gels

List Price:   $899.00
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Sequi-Blot PVDF/Filter Paper Sandwiches #162-0216
Sequi-Blot PVDF/Filter Paper Sandwiches

162-0216
Pkg of 20, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:   $261.00
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Sequi-Blot PVDF/Filter Paper Sandwiches #162-0217
Sequi-Blot™ PVDF/Filter Paper Sandwiches

162-0217
Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for protein sequencing; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:   $568.00
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Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
2212 Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C Click to download
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]
LIT-240 Instruction Manual, Sequi-Blot PVDF Click to download