Trans-Blot® Turbo™ Transfer System

Print

Overview

Superior Transfer Efficiency

Higher sensitivity and better transfer efficiency is seen with the Trans-Blot® Turbo™ system in comparison to other blotting techniques. This data set demonstrates the successful transfer of the 1.25 ng protein band only with the Trans-Blot Turbo system. Serial dilutions of transferrin were separated on a Criterion™ TGX™ 4–20% gel and transferred using four different blotting techniques. All images are adjusted to the same settings.




Trans-Blot® Turbo™ System Tour
Trans-Blot® Turbo™ System Instructional Video


Instructional video to guide you through the features, benefits, and operation of the Trans-Blot Turbo system.

Trans-Blot® Turbo™ System vs. Traditional Blotting Techniques


See how the Trans-Blot Turbo system measures up against traditional blotting techniques.

Trans-Blot® Turbo™ System vs. iBlot


See how the Trans-Blot Turbo system performs compared to the iBlot dry blotting system.

Transfer in 3, 7, or 10 Min

Turbo Transfers with Trans-Blot® Turbo™ Transfer Packs

  • 3-minute protocol — a single Mini-PROTEAN® TGX™ gel (for proteins with MW 5–150 kD) can be transferred in as little as 3 min
  • 7-minute protocol — up to 4 mini or 2 midi gels with mixed-molecular weight proteins (MW 5–150 kD) can be efficiently transferred in 7 min
  • 10-minute protocol — up to 4 mini or 2 midi gels with high-molecular weight proteins (MW 25–300+ kD) can be efficiently transferred in 10 min

E. coli lysate (6 µg) was diluted two-fold. Samples were separated with Mini-PROTEAN TGX gels, transferred with the Trans-Blot Turbo system, stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system. Standards in lane 1 are Precision Plus Protein™ Unstained standards, with a top band of 250 kD MW.


Better Transfer of Different Types of Proteins

2x Protein Transfer

Quantitation performed on equivalent 2-D gels transferred with the Trans-Blot® Turbo™ and the iBlot systems demonstrated twice the number of proteins transferred and detected with the Trans-Blot Turbo system. Rat liver extract was focused on Bio-Rad's ReadyStrip™ 11 cm pH 5–8 IPG strips and separated on AnykD™ Criterion™ TGX™ gels. Duplicate gels were transferred with the Trans-Blot Turbo and iBlot systems, both using manufacturers’ recommended 7 min protocol. The nitrocellulose membranes were subsequently stained with SYPRO Ruby and imaged on a VersaDoc™ MP 4000 system.


Blotter Comparison
 
  Tank Semi-dry iBlot Trans-Blot® Turbo™ system
Speed of transfer •• ••• ••••
Flexibility •• •• •••
Transfer efficiency even for high MW ••• •• •••
Throughput 2 mini gels 6 mini/3 midi gels 2 mini/1 midi gel 4 mini/2 midi gels
Power requirement External power supply (PowerPac™ HC) External power supply (PowerPac™ HC) Integrated power supply Integrated power supply
User interface None None Preset protocols only Preset protocols and easy customization

The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. The system enables blot transfer of protein in as little as 3 minutes without sacrificing performance when compared with traditional tank protein blotting. The Trans-Blot Turbo Transfer Packs include an optimized buffer, membrane, and filter paper combination that provides superior blot transfers when paired with the Trans-Blot Turbo blotting apparatus. Protein blotting consumables are available in a ready-to-use, pre-packaged stack or in bulk quantities that require assembly.

Features and Benefits of the Trans-Blot Turbo Transfer System

  • Rapid blot transfer 3 min transfers of mini or midi gels
  • High throughput — can transfer 1–4 mini or 1–2 midi gels in a single run
  • Greater transfer efficiencyhigher transfer efficiency than other protein blotting methods
  • Flexible design — allows the user to customize transfer conditions and is compatible with traditional semi-dry consumables
  • Environmentally friendly — environmentally safe consumables eliminate disposal cost

Watch this short video to learn more about the Trans-Blot Turbo system.

Applications and Uses of the Trans-Blot Turbo Transfer System

This protein blotting system is a powerful tool in the western blotting workflow, enabling the visualization and identification of proteins for downstream analysis. The Trans-Blot Turbo transfer system can be used in the following applications:

More Information

Trans-Blot Turbo transfer packs come with either PVDF or nitrocellulose membranes:

Select the Trans-Blot Turbo RTA transfer kit for a low-fluorescence membrane option.

Use Bio-Rad’s Precision Plus Protein™ WesternC™ standards and Clarity™ Western ECL substrate for chemiluminescence detection on both film and digital imagers. See how the Trans-Blot Turbo transfer system fits into the V3 Western Workflow™ to streamline your western blotting process.

See the ordering information for Mini-PROTEAN® TGX™ precast gels and Criterion™ TGX™ precast gels to select the best gel for your application.

Account price failed to display. Please try reloading the page. Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at   or send an
email to 

Trans-Blot® Turbo™ Transfer Starter System

1704155
Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit

List Price:
 
$3,150.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot® Turbo™ Mini PVDF Transfer Packs

1704156
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system

List Price:
 
$205.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot® Turbo™ Midi PVDF Transfer Packs

1704157
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, PVDF membrane, for use with Trans-Blot Turbo transfer system

List Price:
 
$241.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs

1704158
Pkg of 10, 7 x 8.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system

List Price:
 
$170.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs

1704159
Pkg of 10, 8.5 x 13.5 cm, precut blotting transfer pack, includes filter paper, buffer, nitrocellulose membrane, for use with Trans-Blot Turbo transfer system

List Price:
 
$211.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Hazardous  
Trans-Blot Turbo RTA Mini Nitrocellulose Transfer Kit
Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit, for 40 blots

1704270
Ready-to-assemble transfer kit includes 40 mini-sized nitrocellulose membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

List Price:
 
$352.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Hazardous  
Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit
Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit, for 40 blots

1704271
Ready-to-assemble transfer kit includes 40 midi-sized nitrocellulose membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

List Price:
 
$460.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Hazardous  
Trans-Blot Turbo RTA Mini PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit, for 40 blots

1704272
Ready-to-assemble transfer kit includes 40 mini-sized PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

List Price:
 
$377.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot Turbo RTA Midi PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit, for 40 blots

1704273
Ready-to-assemble transfer kit includes 40 midi-sized PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

List Price:
 
$512.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Mini LF PVDF Transfer Kit, for 40 blots

1704274
Ready-to-assemble transfer kit includes 40 mini-sized LF PVDF membranes (7 x 8.5 cm), 80 transfer stacks, 1 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

List Price:
 
$377.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot Turbo RTA Midi LF PVDF Transfer Kit
Trans-Blot® Turbo™ RTA Midi LF PVDF Transfer Kit, for 40 blots

1704275
Ready-to-assemble transfer kit includes 40 midi-sized LF PVDF membranes (8.5 x 13.5 cm), 80 transfer stacks, 2 L 5x transfer buffer, and 2 gel trays for wetting and equilibrating membranes and transfer stacks

List Price:
 
$512.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot® Turbo™ Cassette

1704151
Pkg of 1, cassette, to hold 1 midi or up to 2 mini blotting sandwiches, for use with Trans-Blot Turbo transfer system

List Price:
 
$906.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Trans-Blot® Turbo™ Base

1704152
Blotting instrument base, does not include any cassettes

List Price:
 
$2,030.00
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
10026778 Trans-Blot Turbo Transfer System RTA Transfer Kits Quick Start Guide, Rev D Click to download
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
6039 Trans-Blot® Turbo™ Transfer System Brochure, Rev E Click to download [ Add to Cart (Free) ]
6088 V3 Western Workflow Brochure with Gel Doc EZ Imager, Rev B Click to download [ Add to Cart (Free) ]
10020688 Instruction Manual, Trans-Blot® Turbo™ Blotting System, Rev F Click to download
10016505 Instruction Manual, Trans-Blot® Turbo™ Transfer System Quick Start Guide, Rev D Click to download [ Add to Cart (Free) ]
10019593 Instruction Manual, Trans-Blot Turbo Transfer System Transfer Pack Quick Start Guide, Rev E Click to download [ Add to Cart (Free) ]
6487 Transferring High Molecular Weight Proteins on the Trans-Blot Turbo Transfer System Quick Start Guide, Rev A Click to download [ Add to Cart (Free) ]
RP0051 Stain-Free Approach for Western Blotting Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download [ Add to Cart (Free) ]
6147 Robust Transfer of Proteins to Membranes Across a Wide Range of pI and Mass Using the Trans-Blot Turbo System, Rev B Click to download [ Add to Cart (Free) ]
10039859 Trans-Blot Turbo Instrument Protocol, Rev A Click to download
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide, Rev B Click to download [ Add to Cart (Free) ]