Secrets of the Rainforest™ Kit

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Secrets of the Rainforest Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Module 1: Preparing a Bacterial Library  
Bacterial library, E. coli, lyophilized 1
LB nutrient agar tablets 5
Petri dishes, 60 mm, sterile 20
Inoculation loops, sterile 20
Disposable plastic transfer pipets 10
Module 2: Protein Purification  
Ampicillin, lyophilized 1
Arabinose, lyophilized 1
Lysozyme, lyophilized 1
LB nutrient broth tablet 1
Binding buffer 1
Column equilibration buffer 1
Column wash buffer 1
Elution buffer 1
Chromatography columns 8
Inoculation loops, sterile 20
Disposable plastic transfer pipets 40
Micro test tubes, 2.0 ml, clear 30
Cell culture tubes, 15 ml, sterile 25
Sample collection tubes, 5 ml 25
Foam micro test tube holders 8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessories Not Included in Kit:
UV lamps 4–8
Centrifuges 1–2
Recommended (Optional) Accessories:
Incubation oven  
Rocking platform  
Microwave oven  
Secrets of the Rainforest Kit Flowchart
Secrets of the Rainforest Kit Flowchart
Key Kit Features
  • Grow a bacterial library
  • Clone a gene of interest in bacteria
  • Express a protein of interest in bacteria
  • Purify a protein of interest from bacteria
  • Take a protein to market
  • Complete in four 45 min lab sessions
Curriculum Fit
Curriculum Fit
Creating a Bacterial Library and Cloning a Gene

How is a bacterial library created and a gene cloned? A bacterial library is constructed by chopping up the genome of the medicinal plant (the "parent") with a restriction enzyme. Restriction enzymes recognize specific double-stranded DNA sequences and cut each DNA strand, often creating "sticky ends" known as 3' or 5' overhangs. This generates a random assortment of DNA fragments. Some of these DNA fragments from the medicinal plant will contain the gene that encodes the green glowing protein with anticancer properties. When the same restriction enzyme is used to cut (digest) a plasmid (the "destination") it produces an opening in the plasmid with sticky ends that are complementary to the sticky ends of the medicinal plant DNA fragments. Next, the plant DNA fragments are inserted into plasmids by joining (ligating) their sticky ends and covalently bound together by a DNA ligase enzyme. The resulting plasmids contain random assortments of DNA fragments derived from the genome of the medicinal plant. The plasmids are transformed into the bacteria, E. coli. When transformed bacteria divide, the plasmid is also replicated. In the best-case scenario, some of the transformed bacterial cells will contain copies of the plasmid containing the plant gene, which encodes the protein of interest.

Secrets of the Rainforest colonies and GFP separation on a series of hydrophobic interaction chromatography (HIC) columns.

Secrets of the Rainforest colonies and GFP separation on a series of hydrophobic interaction chromatography (HIC) columns.

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The Secrets of the Rainforest™ Kit provides a biotechnology curriculum that takes students on an adventure starting in the rainforest in the Andes, continuing to a biotechnology company engaged in developing new pharmaceutical compounds, and finally to the Food and Drug Administration.

Features and Benefits

  • Applies the principles of DNA restriction analysis, bacterial transformation, and protein purification
  • Introduces your students to the world of commercial biotechnology

With this kit, students are able to:

  • Grow a bacterial library
  • Clone a gene of interest in bacteria
  • Express a protein of interest in bacteria
  • Purify a protein of interest from bacteria
  • Simulate taking a protein to market

How It Works

In the hands-on portion of the exercise, students streak out the bacterial library, grow the bacteria, and select the colonies that have acquired the green glowing trait from a background of nonglowing bacteria. A culture of bacteria is grown to scale up production of the green glowing protein, which is then purified by column chromatography. The actual source of this fluorescent protein is the bioluminescent jellyfish Aequorea victoria.

This practical activity is followed by dry-lab lessons in which the purified green protein is put through the drug discovery process. This includes learning about Food and Drug Administration regulations, animal testing, marketing practices for introducing a new drug, financing, ethical issues, and student presentations. Students examine the needs and viewpoints of advocacy groups, the biotechnology industry, and the FDA. They consider ethical, economic, social, and practical issues, and develop possible strategies for what they feel are the best outcomes.

More Information

  • Four-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Module 1: Preparing a Bacterial Library  
  Bacterial library, E. coli, lyophilized 1
  LB nutrient agar tablets 5
Petri dishes, 60 mm, sterile 20
Inoculation loops, sterile 20
Disposable plastic transfer pipets 10
Module 2: Protein Purification  
Ampicillin, lyophilized 1
Arabinose, lyophilized 1
Lysozyme, lyophilized 1
LB nutrient broth tablet 1
Binding buffer 1
Column equilibration buffer 1
Column wash buffer 1
Elution buffer 1
  Chromatography columns 8
Inoculation loops, sterile 20
Disposable plastic transfer pipets 40
Micro test tubes, 2.0 ml, clear 30
Cell culture tubes, 15 ml, sterile 25
Sample collection tubes, 5 ml 25
Foam micro test tube holders 8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
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Secrets of the Rainforest Kit

1660006EDU
Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only

0 1660006EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0006EDU_0921121040390.xml 1660006EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:24 1660006edu-secrets-rainforest-kit 1660006EDU Secrets of the Rainforest Kit Secrets of the Rainforest Kit Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0006EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0006EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0006EDU_feature.jpg RepEx 166-0006EDU Bacterial library screening kit, classroom study kit, includes bacterial library and protein purification modules, curriculum, and more, for 32 students; education use only 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 1660006, 1660006EDU, secrets, of, the, rainforest, bacterial, library, screening, protein, purification, modules, curriculum, cloning, expression, science, education, products, classroom false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660006EDU&CartQuote=QT&quantity= false true false $295.00 true %24236.00 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660006EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660006EDU&quantity= 1660006EDU
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Bacterial Library

1660411EDU
Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only

1660411EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0411EDU_0921121040444.xml 0 1660411EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:24 1660411edu-bacterial-library 1660411EDU Bacterial Library Bacterial Library Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0411EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0411EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0411EDU_feature.jpg RepEx 166-0411EDU Pkg of 1, E. coli library, for use with Secrets of the Rainforest kit (#166-0006EDU); education use only Bacterial Library, secrets of the Rainforest kit, E. coli library, classroom refill kit 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 <p>The Bacterial Library is for use with the Secrets of the Rainforest&trade; Kit (#166-0006EDU). The <em>E. coli</em> library is supplied lyophilized. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated 166-0411EDU_0921121040444_en.html /webroot/web/html/lse/products/SKUs/autopopulated/166-0411EDU_0921121040444_en.html false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660411EDU&CartQuote=QT&quantity= false true false $46.00 true %2436.80 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660411EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660411EDU&quantity= 1660411EDU
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Lyophilized Components Refill Pack

1660015EDU
Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only

1660015EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0015EDU_0921121040371.xml 1 1660015EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:24 1660015edu-lyophilized-components-refill-pack 1660015EDU Lyophilized Components Refill Pack Lyophilized Components Refill Pack Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0015EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0015EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0015EDU_feature.jpg Lyophilized Components Refill Pack #166-0015EDU RepEx Lyophilized Components Refill Pack #166-0015EDU Consumable reagents for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes ampicillin, arabinose, LB nutrient broth tablet, lysozyme; education use only 1660015, 1660015EDU, lyophilized, components, refill, pack, gfp, chromatography, secrets, of, the, rainforest, ampicillin, arabinose, lb, nutrient, broth, tablet, lysozyme, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 <p>The Lyophilized Components Refill Package contains consumable reagents for the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest&trade; Kit (#166-0006EDU). The package includes ampicillin, arabinose LB nutrient broth tablet, and lysozyme. This product is for education use only. </p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0015EDU_0921121040371_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0015EDU_0921121040371_en.html 1158 <b>Refill contains sufficient lyophilized reagents for 8 student workstations (2&ndash;4 students per workstation)</b> &nbsp; 1 0 2453 Ampicillin, lyophilized 1 1 0 1065 Arabinose, lyophilized 1 1 0 1076 Lysozyme, lyophilized 1 1 0 1075 LB nutrient broth tablet 1 1 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&CartQuote=QT&quantity= false true false $122.00 true %2497.60 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660015EDU&quantity= 1660015EDU
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GFP Chromatography Buffers Refill Pack

1660016EDU
Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only

1660016EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml 3 1660016EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0016EDU_0921121040480.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:24 1660016edu-gfp-chromatography-buffers-refill-pack 1660016EDU GFP Chromatography Buffers Refill Pack GFP Chromatography Buffers Refill Pack Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0016EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0016EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0016EDU_feature.jpg GFP Chromatography Buffers Refill Pack #166-0016EDU RepEx GFP Chromatography Buffers Refill Pack #166-0016EDU Consumable buffers for the GFP chromatography and Secrets of the Rainforest kits (#166-0005EDU, 166-0006EDU), includes binding, equilibration, wash, and elution buffers; education use only 1660016, 1660016EDU, gfp, chromatography, buffers, refill, pack, consumables, replacement, buffer, secrets, of, the, rainforest, binding, equilibration, wash, elution, reagent, reagents, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 <p>Green Fluorescent Protein Chromatography Kit Lyophilized Components Refill Package contains consumable buffers for the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest&trade; Kit (#166-0006EDU). The package includes binding, equilibration, wash, and elution buffers. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0016EDU_0921121040480_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0016EDU_0921121040480_en.html 1155 <b>Refill contains sufficient buffers for 8 student workstations (2&ndash;4 students per workstation)</b> &nbsp; 1 0 1066 Binding buffer 1 1 0 1454 Column equilibration buffer 1 1 0 1461 Column wash buffer 1 1 0 1072 Elution buffer 1 1 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&CartQuote=QT&quantity= false true false $101.00 true %2480.80 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660016EDU&quantity= 1660016EDU
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HIC Chromatography Columns and Caps

1660413EDU
Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only

1660413EDU /templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml 2 1660413EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit/166-0413EDU_0921121040426.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:24 1660413edu-hic-chromatography-columns-caps 1660413EDU HIC Chromatography Columns and Caps HIC Chromatography Columns and Caps Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/166-0413EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/166-0413EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/166-0413EDU_feature.jpg HIC Chromatography Columns and Caps #166-0413EDU RepEx HIC Chromatography Columns and Caps #166-0413EDU Pkg of 8, hydrophobic interaction chromatography column with cap, for use with Biotechnology Explorer classroom kits; education use only 1660413, 1660413EDU, hic, chromatography, columns, and, caps, refill, hydrophobic, interaction, column, biotechnology, explorer, classroom, science, education, products 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/pGLO-GFP-Kits/Secrets-of-the-Rainforest-Kit N 0 <p>Hydrophobic Interaction Chromatography Columns and Caps, package of eight, are for use with Biotechnology Explorer&trade; classroom kits. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-0413EDU_0921121040426_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-0413EDU_0921121040426_en.html 2728 HIC columns 8 1 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&CartQuote=QT&quantity= false true false $72.00 true %2457.60 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1660413EDU&quantity= 1660413EDU
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Poly-Prep Columns

7311550EDU
Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only

7311550EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/731-1550EDU_0921121032943.xml 4 7311550EDU 09/21/09 12:10 PM N en 09/21/19 12:10 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSE 2017-11-14 07:07:16 7311550edu-poly-prep-columns 7311550EDU Poly-Prep Columns Poly-Prep Columns Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/731-1550EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/731-1550EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/731-1550EDU_feature.jpg Poly-Prep Columns #731-1550EDU RepEx Poly-Prep Columns #731-1550EDU Pkg of 50, conical 0.8 x 4 cm empty polypropylene column, 9 cm high, 2 ml bed volume, 10 ml of sample volume; education use only 7311550, 7311550EDU, poly-prep, columns, conical, empty, polypropylene, column, small-scale, gravity-flow, purification, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Chromatography N 0 <p>Poly-Prep<sup>&reg;</sup> Columns are conical 0.8 x 4 cm empty polypropylene columns, 9 cm high, with a 2 ml bed volume and a sample volume of 10 ml. They are sold as a package of 50. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 731-1550EDU_0921121032943_en.html /webroot/web/html/lse/products/SKUs/autopopulated//731-1550EDU_0921121032943_en.html 2709 Poly-Prep<sup>&reg;</sup> columns, empty, 0.8 x 4 cm 50 1 0 7317005 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-7005_0921120638974.xml 7311555 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Columns/731-1555_0921120639354.xml 1560131 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/156-0131_0921120658411.xml false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&CartQuote=QT&quantity= false true false $125.00 true %24100.00 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7311550EDU&quantity= 7311550EDU
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Macro-Prep HIC Support

1560080EDU
100 ml, weakly hydrophobic methyl chromatography support; education use only

1560080EDU /templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml 5 1560080EDU <?xml version="1.0" encoding="UTF-8"?> <Deployments> <Node Name="QA"> <Deployment Id="StandardMetadata.jd0nawbijd0nawbj" Class="com.interwoven.livesite.lscs.model.LSCSDeploymentImpl" Instance="jd0nawbijd0nawbj"> <Name>StandardMetadata</Name> <TargetEnviroment>QA</TargetEnviroment> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Deployment> <Vpath>/default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Equipment-and-Supplies/Chromatography/156-0080EDU_0921121032560.xml</Vpath> <LastExecutionTime Class="java.util.Date" Month="2" DayOfMonth="2" DayOfWeek="5" Year="2018" Hour="23" Minute="0" Second="5" ZoneOffsetMillis="0" Time="1517612405070">Fri Feb 02 23:00:05 UTC 2018</LastExecutionTime> </Node> </Deployments> 09/21/09 12:10 PM true true internet/product_SKU LSE N en 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA 2017-11-14 07:07:15 1560080edu-macro-prep-hic-support 1560080EDU Macro-Prep HIC Support Macro-Prep HIC Support 100 ml, weakly hydrophobic methyl chromatography support; education use only 100 ml, weakly hydrophobic methyl chromatography support; education use only 100 ml, weakly hydrophobic methyl chromatography support; education use only /webroot/web/images/lse/products/pglo_gfp_kits/sku_thumb/global/156-0080EDU_tn.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_view/global/156-0080EDU_view.jpg /webroot/web/images/lse/products/pglo_gfp_kits/sku_feature/global/156-0080EDU_feature.jpg Macro-Prep HIC Support #156-0080EDU RepEx Macro-Prep HIC Support #156-0080EDU 100 ml, weakly hydrophobic methyl chromatography support; education use only 1560080, 1560080EDU, macro-prep, methyl, hic, support, hydrophobic, interaction, chromatography, media, medium, resin, protein, separation, purification, 50um, 50, particle, size, science, education, products, classroom 09/21/09 12:10 PM 09/21/19 12:10 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSE /LSE/Equipment-and-Supplies/Chromatography N 0 <p>Macro-Prep<sup>&reg;</sup> HIC Support, 100 ml, is a weakly hydrophobic methyl chromatography support. The product is for use with the Green Fluorescent Protein Chromatography Kit (#166-0005EDU) and the Secrets of the Rainforest&trade; Kit (#166-0006EDU). This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 156-0080EDU_0921121032560_en.html /webroot/web/html/lse/products/SKUs/autopopulated//156-0080EDU_0921121032560_en.html 2708 Macro-Prep<sup>&reg;</sup> HIC support, 100 ml 1 1 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&CartQuote=QT&quantity= false true false $578.00 true %24462.40 /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1560080EDU&quantity= 1560080EDU
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Number Description Options
3051 Secrets of the Rain Forest Kit, Biotechnology Explorer, Quick Guide Click to download
4006098 Instruction Manual, Secrets of the Rain Forest Kit, Biotechnology Explorer, Rev B Please login to download this document

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