ROX Passive Reference Dye

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Many real-time PCR thermal cyclers require an internal ROX reference dye for fluorescent signal normalization and correction of well-to-well optical variations. Bio-Rad's ROX Passive Reference Dye allows PCR reagents that do not contain ROX to be used on all ROX-dependent real-time PCR systems.

As the excitation optics of different instrument platforms vary, the optimal ROX reference dye concentration must be matched to each real-time PCR cycler. This ROX reference dye is formulated as a 50x concentrated stock solution for use on the following instruments:

  • ABI PRISM 7000, 7300, 7700, and 7900 systems

This ROX reference dye should be treated as a 750x concentrated solution for systems that use 580–585 nm excitation for passive reference dye, such as the following instruments:

  • Stratagene Mx4000, Mx3000P, or Mx3005P systems
  • ABI PRISM 7500 sequence detection system

Note: An internal ROX reference dye is not required for use with any Bio-Rad real-time PCR detection system.

Storage at –20°C Guaranteed for 12 months at –20°C in a constant temperature freezer (multiple freeze thaws not recommended)
Storage at +4°C Guaranteed for 6 months at 4°C (protected from light)

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ROX Passive Reference Dye

1725858
0.5 ml, passive reference dye for use on ROX-dependent real-time PCR instruments

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