ReadyPrep™ Sequential Extraction Kit

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ReadyPrep Sequential Extraction Kit

Overview



The ReadyPrep sequential extraction kit is used to extract proteins of differing solubility from cell lysates. Based on the work of Molloy et al. (1998) and Herbert et al. (1998), the kit uses a series of three extraction buffers, each differing in their detergent and chaotrope concentrations (Rabilloud et al. 1997). The insoluble residues from each extraction step are further extracted with reagents of increasingly stronger solubilizing power.

The ReadyPrep sequential extraction kit:

  • Yields fractions that are suitable for direct analysis by a number of methods, including 2-D electrophoresis
  • Can be carried out in a single microcentrifuge tube, minimizing protein loss
  • Results in manageable 2-D protein patterns for simplified protein identification and gel matching
  • Generates fractions with high protein loads to enable visualization of low-abundance proteins

 

References
Molloy, MP, et al. (1998). Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19, 837–844.
Herbert, BR, et al. (1998). Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Electrophoresis 19, 845–851.
Rabilloud, T, et al. (1997). Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 18, 307–316.

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ReadyPrep™ Sequential Extraction Kit

1632100
5–15 preps, protein preparation and fractionation kit, for differential solubilization with SB 3-10, includes 3 premixed reagents, TBP, 3 protocols

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ReadyPrep™ Sequential Extraction Kit Reagent 1

1632102
50 ml, 40 mM Tris base, for protein preparation and fractionation buffers; component of kit #163-2100

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ReadyPrep™ Sequential Extraction Kit Reagent 2

1632103
10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100

2 1632103 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2103_0921120738361.xml 1632103 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:59 1632103-readyprep-sequential-extraction-kit-reagent-2 1632103 ReadyPrep Sequential Extraction Kit Reagent 2 ReadyPrep™ Sequential Extraction Kit Reagent 2 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2103_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2103_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2103_feature.jpg preview RepEx 163-2103 10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Protein-Sample-Preparation/Protein-Fractionation N 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632103&CartQuote=QT&quantity= false true false $51.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632103&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632103&quantity= 1632103
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ReadyPrep™ Sequential Extraction Kit Reagent 3

1632104
10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100

3 1632104 /templatedata/internet/product_SKU/data/LSR/Protein-Sample-Preparation/Protein-Fractionation/163-2104_0921120738380.xml 1632104 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:59 1632104-readyprep-sequential-extraction-kit-reagent-3 1632104 ReadyPrep Sequential Extraction Kit Reagent 3 ReadyPrep™ Sequential Extraction Kit Reagent 3 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 /webroot/web/images/lsr/products/protein_sample_preparation/sku_thumb/global/163-2104_tn.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_view/global/163-2104_view.jpg /webroot/web/images/lsr/products/protein_sample_preparation/sku_feature/global/163-2104_feature.jpg preview RepEx 163-2104 10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Protein-Sample-Preparation/Protein-Fractionation N 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632104&CartQuote=QT&quantity= true true false $51.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632104&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1632104&quantity= 1632104
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Number Description Options
4006167 Instruction Manual, ReadyPrep™ Sequential Extraction Kit, Rev B Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download [ Add to Cart (Free) ]

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