ReadyPrep Reagents for Extraction and Solubilization

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ReadyPrep Buffers
Product Name Description Volume Catalog #
ReadyPrep 2-D rehydration/sample buffer 1 7 M urea, 2 M thiourea, 1% (w/v) ASB-14 detergent, 40 mM Tris base, 0.001% Bromophenol Blue 10 ml 163–2083
ReadyPrep 2-D starter kit rehydration/sample buffer 8 M urea, 2% CHAPS, 50 mM DTT, 0.2% Bio-Lyte 3/10 ampholyte, 0.001% Bromophenol Blue 10 ml 163–2106
ReadyPrep sequential extraction kit reagent 1 40 mM Tris base 50 ml 163–2102
ReadyPrep sequential extraction kit reagent 2 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte 10 ml 163–2103
ReadyPrep sequential extraction kit reagent 3 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3–10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte 10 ml 163–2104
Protein Solubilization buffer (PSB) Urea, thiourea, CHAPS, NDSB 201 50 ml 163–2145



A number of ReadyPrep kits are available for total protein or targeted protein extraction (fractionation). Some of the premixed buffer and reagent components of these kits are also available separately for use in customized protein extraction and solubilization protocols.

ReadyPrep™ 2-D Rehydration/Sample Buffer 1

163-2083
10 ml, protein solubilization reagent, includes 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 0.001% Bromophenol Blue

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ReadyPrep™ 2-D Starter Kit Rehydration/Sample Buffer

163-2106
10 ml, protein sample buffer, includes 8 M urea, 2% CHAPS, 50 mM DTT, 0.2% Bio-Lyte® 3/10 ampholyte, 0.001% Bromophenol Blue

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ReadyPrep Sequential Extraction Kit Reagent 1

163-2102
50 ml, 40 mM Tris base, for protein preparation and fractionation buffers; component of kit #163-2100

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ReadyPrep Sequential Extraction Kit Reagent 2

163-2103
10 ml, protein preparation and fractionation reagent, includes 8 M urea, 4% CHAPS, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100

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ReadyPrep Sequential Extraction Kit Reagent 3

163-2104
10 ml, protein preparation and fractionation reagent, includes 5 M urea, 2 M thiourea, 2% CHAPS, 2% SB 3-10, 40 mM Tris, 0.2% Bio-Lyte 3/10 ampholyte; component of kit #163-2100

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Protein Solubilization Buffer (PSB)

163-2145
Pkg of 1, strongly chaotropic protein solubilization buffer, contains NDSB 201, urea, thiourea, and CHAPS, makes 50 ml of solution

List Price:   $144.00
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ReadyPrep Proteomics Grade Water

163-2091
500 ml, ultrapure water for use with ReadyPrep fractionation and general purpose cleanup kits

List Price:   $49.00
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Number Description Options
4006167 Instruction Manual, ReadyPrep Sequential Extraction Kit, Rev B Click to download