ReadyPrep™ Protein Extraction Kit (Soluble/Insoluble)

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ReadyPrep Protein Extraction Kit (Soluble/Insoluble)




The ReadyPrep protein extraction kit (soluble/insoluble) separates total protein samples into two fractions based on differential protein solubility. It generates a soluble fraction containing hydrophilic proteins and an insoluble fraction containing hydrophobic proteins. Proteins in the insoluble fraction are eventually resolubilized in ReadyPrep 2-D rehydration/solubilization buffer 1, a strongly chaotropic solution containing the zwitterionic detergent ASB-14. This simple fractionation strategy results in less complex samples and leads to improved identification of low-abundance proteins and a better overall view of the proteome.

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ReadyPrep™ Protein Extraction Kit (Soluble/Insoluble)

1632085
20 preps, protein preparation and fractionation kit for whole proteome analysis in one fractionation step, includes strong detergent ASB-14

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ReadyPrep™ 2-D Rehydration/Sample Buffer 1

1632083
10 ml, protein solubilization reagent, includes 7 M urea, 2 M thiourea, 1% ASB-14, 40 mM Tris, 0.001% Bromophenol Blue

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Number Description Options
4110158 Instruction Manual, ReadyPrep™ Protein Extraction Kit (Soluble/Insoluble), Rev A Click to download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download

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