Affi-Gel Heparin Gel

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Overview

Affinity Media Guide
  Matrix Functional Group Specificity Capacity Working pH Pressure Limit Applications
Ready-to-Use Affinity Media
UNOsphere SUPrA Highly cross-linked polyacrylamide polymer Recombinant protein A Immunoglobulins/ Antibodies 25 to 30 mg/ml 3–11 100–600 cm/hr Monoclonal Antibody purification
Profinity IMAC Pressure-stable polymer based on UNOsphere beads IDA, provided charged with Ni2+ and uncharged Histidine ≥15 mg/ml* 1–14 100 psi
(6.8 bar)
Purification of recombinant His-tagged protiens; can be charged with other transition metals
Profinity GST Pressure-stable polymer based on UNOsphere beads Immobilized glutathione GST-tagged proteins ≥10 mg/ml 1–14 45 psi
(3.1 bar)
Purification of recombinant GST-tagged proteins
Profinity eXact Crosslinked 6% agarose Subtilisin protease Subtilisin prodomain ≥3 mg/ml tag-free protein 2–10 10 psi
(minus system pressure)
Generation of native, tag-free protein by on-column purification and cleavage
Affi-Gel protein A Crosslinked agarose Protein A 2 mg/ml IgG   2–10 15 psi
(1 bar)
Purification of IgG from ascites, serum, and culture fluid; with MAPS buffer system, purification of 10 mg mouse IgG1 per ml of gel is possible
Affi-Prep protein A Pressure stable polymer Protein A 2 mg/ml IgG   2–10 1,000 psi
(70 bar)
Purifies IgG from ascites, serum, and culture fluid; pressure-stable support for process-scale applications
Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA 1.9 mg/ml Albumin; general ≥11 mg/ml 2–10 15 psi
(1 bar)
Binds many nucleotide-requiring enzymes, albumin, and other proteins
DEAE Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and DEAE Albumin and serum proteins 0.14 ml serum/ml gel 2–10 15 psi
(1 bar)
Purifies protease-free IgG from ascites, serum, and culture fluid with minimal sample preparation
CM Affi-Gel Blue Crosslinked agarose Cibacron Blue F3GA and CM Albumin and serum proteins 0.17–0.5 ml serum/ml Gel 2–11 15 psi
(1 bar)
Produes albumin and protease-free antibody preparation from serum without prior dialysis
Affi-Prep polymyxin Pressure-stable polymer Polymyxin 2–4 mg/ml Endotoxins >5 mg/ml 2–10 1,000 psi
(70 bar)
Endotoxin removal
Affi-Gel boronate Polyacrylamide gel Boronate 1.05±0.15 cis-diols
meq/g
130 µmol sorbitol/ml 2–10 15 psi
(1 bar)
Adsorption of cis-hydroxyl-containing molecules, including sugars, nucleotides, and glycopeptides
Activated Media for Spontaneous Ligand Immobilization
Profinity epoxide Pressure-stable polymer based on UNOsphere beads Epoxy group Nucleophiles;amino, thiol, -COOH 36–40 mg/ml IgG 1–14 Up to 80 psi
(5.5 bar)
Activated matrix for the immobilization of various ligands (for example, protein A, StrepTactin, and immunoglobulins)
Affi-Gel 10 Crosslinked agarose N-hydroxy-succinimide ≥10 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pl 6.5–11
Affi-Gel 15 Crosslinked agarose N-hydroxy-succinimide ≥9 µmol/ml -NH2 35 mg/ml 3–11 15 psi
(1 bar)
For coupling proteins with pl <6.5
Affi-Gel Hz Crosslinked agarose Hydrazide Oxidized carbohydrates 1–5 mg/ml 2–10 15 psi
(1 bar)
Immobilization of immunoglobulins and other glycoproteins via carbohydrate moieties
Affinity Media Using Carbodiimide Activation
Affi-Gel 102 Crosslinked agarose -NH2
16 ± 4 meq/ml
-COOH 40 mg 2–11 15 psi
(1 bar)
Carbodiimide coupling of carboxyl-containing ligand

* Refer to Bulletin 3193 for purification conditions.

Affi-Gel heparin gel is a ready-to-use support for the purification of a range of proteins such as coagulation factors, other plasma proteins, polynucleotide polymerases, nucleases, lipases, lipoproteins, and proteases. Heparin binds a variety of enzymes and other proteins, either ionically or by other specific enzyme-inhibitor (or enzyme-activator) interactions.

Features of Affi-Gel heparin gel include:

  • Heparin content of ≥0.6 mg/ml
  • Human antithrombin III binding capacity of ≥1.2 mg/ml
  • Linear flow rates of 10–20 cm/hr
Affi-Gel Heparin Gel

153-6173
40 ml, ready-to-use affinity gel

List Price:   $630.00
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LIT301 Instruction Manual, Affi-Gel Heparin, Rev B Click to download