Ready-Prep %CDT by HPLC™

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Ready-Prep %CDT by HPLC™

The Bio-Rad Ready-Prep %CDT by HPLC™ kit is designed for reliable, fast and easy determination of %CDT in human serum. Useful for routine screening and confirmatory testing, this assay features rapid, convenient sample preparation, detection of genetic transferrin variants and excellent correlation with a reference method for HPLC. The sample preparation reagents are provided in a premixed, ready-to-use, liquid form, now five individual reagents are replaced by one. In addition, no reagent mixing procedure before each test run required. Two kit configurations will allow for testing of 100 or 300 samples: The 100 test kit will include 100 already pre-filled Ready-Prep vials, and the 300 test kit will include a pre-mixed Ready-Prep Reagent bottle for use with either manual or automated dispensing procedures.

To assist laboratories in standardization, a two-level calibrator set and two-level control set are now available. A new, fast method that more than doubles the hourly throughput is available to customers using modular HPLC equipment.

Currently, CDT (carbohydrate deficient transferrin) is the most specific biochemical marker for the detection of alcohol abuse, and for monitoring abstinence during treatment. Daily consumption of 50 - 80 grams ethanol for one week or longer results in elevated levels of %CDT. The %CDT by HPLC kit measures the relative amounts of single transferrin isoforms (e.g. disialotransferrin) to total transferrin.

Test characteristics:

  • Principle: Gradient HPLC method (Anion-exchange)
  • Duration of the chromatography: 10 min (Standard method) / 4.5 min (Fast method)
  • Sample volume: 150 µL serum or plasma
  • Flow rate: 1.4 mL/min (Standard method) / 2.6 mL/min (Fast method)
  • UV Detection: 460 nm


Ready-Prep %CDT by HPLC™, 100 tests
Ready-Prep %CDT by HPLC™, 100 tests

195-6670
Sample preparation reagents include 100 pre-filled Ready-Prep vials for use with manual dispensing procedure (100 tests)

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Ready-Prep %CDT by HPLC™, 300 tests
Ready-Prep %CDT by HPLC™, 300 tests

195-6671
Sample preparation reagents include a pre-mixed Ready-Prep reagent bottle for use with automated dispensing procedure (300 tests)

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Analytical Cartridge for %CDT by HPLC Reagent Kit
Analytical Cartridge for %CDT by HPLC Reagent Kit

195-6662
Analytical cartridge to assay %CDT by HPLC (600 injections)

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Holder for Analytical and Guard Cartridge for %CDT by HPLC Reagent Kit
Holder for Analytical and Guard Cartridge for %CDT by HPLC Reagent Kit

195-6663
Holder for analytical and guard cartridges used for %CDT by HPLC assay

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Calibrator Set (bi-level) for %CDT by HPLC Reagent Kit
Calibrator Set (bi-level) for %CDT by HPLC Reagent Kit

195-6650
Set of bi-level calibrators for %CDT by HPLC assay (2 x 1 mL)

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Test Mix for %CDT by HPLC Reagent Kit
Test Mix for %CDT by HPLC Reagent Kit

195-6665
Test mixture for %CDT by HPLC assay (1 mL)

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Control Set (bi-level) for %CDT by HPLC Reagent Kit
Control Set (bi-level) for %CDT by HPLC Reagent Kit

195-6669
Set of bi-level controls for %CDT by HPLC assay (2 x 1 mL)

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