RAPID'Staph Medium

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Overview


Key Benefits
  • Complete results in 24 hr
  • Selective
  • Easy alternative confirmation

Protocol


Validation

Validated and Adapted for Food Detection

RAPID’Staph protocol is certified AFNOR Certification, according to the ISO 16140 requirements for the detection of Staphylococcus aureus. (No. BRD 07/9-02/05) and by AOAC-RI (No. 080602).

Intended Use

RAPID’Staph medium is a selective agar used for the direct enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) at 37°C in 24 hr in products intended for human or animal consumption or in environmental samples. Presumptive colonies can be confirmed using one of the following:

  • Pastorex®Staph-Plus latex test with results within minutes
  • Spot inoculate on Baird-Parker + RPF agar to confirm many colonies on the same plate

Principle

The principle of RAPID’Staph medium is based on an optimized Baird-Parker formula to guarantee the detection and enumeration of S. aureus in 24 hr. Typical staphylococci produce gray to black colonies that are shiny and convex (1–2 mm) with a clear halo around the colony (2–5 mm) due to proteolysis of the egg yolk.

Assay type Chromogenic media
Organism Staphylococcus aureus
RAPID'Staph Agar #356-3960
RAPID'Staph Agar

356-3960
90 mm x 20 dishes

List Price:   $42.00
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RAPID'Staph Agar Base, dehydrated #356-4704
RAPID'Staph Agar Base, dehydrated

356-4704
500 g

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Egg Yolk with Potassium Tellurite Supplement #355-4201
Egg Yolk with Potassium Tellurite Supplement

355-4201
5 ml x 1 vial

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Egg Yolk with Potassium Tellurite Supplement #355-4205
Egg Yolk with Potassium Tellurite Supplement

355-4205
25 ml x 1 bottle

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Sulfamethazine Solution (0.2%) Supplement #356-2682
Sulfamethazine Solution (0.2%) Supplement

356-2682
2.5 ml x 1 vial

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Number Description Options
5533 Evaluation of RAPID'Staph Medium for Enumeration of Coagulase-Positive Staphylococcus aureus in Selected Foods, Rev A Click to download
FSD_16288 RAPID’Chromogenic Methods for Food and Environmental Testing Brochure, Rev H Click to download
TS_RapidStaph TS_RapidStaph Click to download