RAPID'Staph Medium

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Overview


Key Benefits
  • Complete results in 24 hr
  • Selective
  • Easy alternative confirmation

Protocol
Enumeration of Coagulase-positive Staphylococci
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/09-02/05
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID’Staph protocol is certified by AFNOR Certification, according to the ISO 16140 requirements for the detection of Staphylococcus aureus. (No. BRD 07/09-02/05) and by AOAC-RI (No. 080602).

Intended Use

RAPID’Staph medium is a selective agar used for the direct enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) at 37°C in 24 hr in products intended for human or animal consumption or in environmental samples. Presumptive colonies can be confirmed using one of the following:

  • Pastorex®Staph-Plus latex test with results within minutes
  • Spot inoculate on Baird-Parker + RPF agar to confirm many colonies on the same plate

Principle

The principle of RAPID’Staph medium is based on an optimized Baird-Parker formula to guarantee the detection and enumeration of S. aureus in 24 hr. Typical staphylococci produce gray to black colonies that are shiny and convex (1–2 mm) with a clear halo around the colony (2–5 mm) due to proteolysis of the egg yolk.

Assay type Chromogenic media
Organism Staphylococcus aureus
RAPID'Staph Agar #356-3960
RAPID'Staph Agar

356-3960
90 mm x 20 dishes

List Price:   $42.00
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RAPID'Staph Agar Base, dehydrated #356-4704
RAPID'Staph Agar Base, dehydrated

356-4704
500 g

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Egg Yolk with Potassium Tellurite Supplement #355-4201
Egg Yolk with Potassium Tellurite Supplement

355-4201
5 ml x 1 vial

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Egg Yolk with Potassium Tellurite Supplement #355-4205
Egg Yolk with Potassium Tellurite Supplement

355-4205
25 ml x 1 bottle

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Sulfamethazine Solution (0.2%) Supplement #356-2682
Sulfamethazine Solution (0.2%) Supplement

356-2682
2.5 ml x 1 vial

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Number Description Options
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
FSD_16288 RAPID’Chromogenic Methods for Food and Environmental Testing Brochure, Rev G Click to download
FSD_16054 RAPID’Staph Agar Product Information Click to download
PF_259 Gélose RAPID’Staph Information produit (Tous pays, sauf USA) Click to download
TS_RapidStaph_en RAPID'Staph Agar Technical Sheet v. 3 (all countries except U.S. and Canada) Click to download
FT_Rstaph_fr RAPID’Staph/Gélose v.3 (Tous pays, sauf USA and Canada) Click to download
TS_RapidStaph RAPID'Staph Agar Technical Sheet v. 3 (U.S. and Canada) Click to download
5533 Evaluation of RAPID'Staph Medium for Enumeration of Coagulase-Positive Staphylococcus aureus in Selected Foods, Rev A Click to download