RAPID'Listeria spp. Medium

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Overview


Key Benefits
  • Economical: just one broth and one plate are required per sample
  • Rapid response after just 24 hr of incubation for all Listeria species after enrichment
  • Validated high specificity and selectivity
  • Easy-to-read chromogenic reaction
  • All Listeria spp. are blue
Protocol
Detection of Lysteria spp.
Validation

Validated and Adapted for Food Detection

AFNOR
BRD 07/12-12/06
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID'Listeria spp. method is certified by AFNOR certification according to the ISO 16140 for the detection of Listeria spp. (No. BRD 07/12-12/06) and AOAC-RI validated (No. 080701)



Intended Use

RAPID'Listeria spp. Medium is an affordable selective chromogenic agar for the detection of Listeria spp. in food and environmental samples. 

Key Benefits

  • Fast and cost effective
    • Only 1 broth and 1 plate are required per sample
    • Results after just 24 hr of incubation for all Listeria species after 24 hr enrichment
  • High specificity and selectivity
    • Easy-to-read chromogenic reaction based on the specific b-glucosidase activity of Listeria
    • Blue color for all Listeria spp.
    • Reading and differentiation are easy due to the contrast between blue-green Listeria colonies and the whitish agar
    • The selectivity and high nutrient content of the medium prevent growth of interfering flora
  • Validation
    • Certified NF VALIDATION according to the ISO 16140 standard
    • Validated by AOAC
  • Easy Confirmation
    • Single spot inoculation on RAPID'L.mono agar (+Gram and catalase tests) or on Palcam has been validated for confirmation

Principle

Identification of Listeria spp. using RAPID'Listeria spp. chromogenic medium is based on the detection of β‑D‑glucosidase activity by a chromogenic substrate. Listeria colonies are blue to bluish-green. The medium is made selective by the combined action of lithium chloride and an antibiotic mixture inhibiting Bacillus and all interfering flora.

Listeria spp. Monitoring: Preventing Listeria monocytogenes Contamination

When Listeria gets into food, it often comes from environmental contamination. Finding sources of contamination by Listeria spp. in the processing plant is the first step towards preventing Listeria monocytogenes contamination of food.

A proactive, aggressive environmental monitoring program is needed as a first step in preventing contamination. The RAPID'Listeria spp. method of environmental monitoring can improve the chances of finding where Listeria spp. exist in the environment and reducing the risk of food contamination.

RAPID'Listeria spp. Chromogenic Assay

Enzymatic Activity
Listeria spp.
β-D-Glucosidase +
     

Ordering Information

RAPID'Listeria spp. Medium is offered as a dehydrated agar base (3564744). When preparing the medium from the dehydrated base, RAPID'Listeria spp. Supplement 1 (3564745) and Supplement 2 (3564746) need to be added to the molten agar prior to pouring plates.

Assay type Chromogenic media
Organism Listeria spp.
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RAPID'Listeria spp. Agar Base #356-4744
RAPID'Listeria spp. Agar Base, dehydrated

3564744
Pkg of 1, 500 g of dehydrated agar base

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$567.00
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RAPID'Listeria spp. Supplement 1 #356-4745
RAPID'Listeria spp. Supplement 1

3564745
Pkg of 10, vials of supplement 1 for 'RAPID´L.mono Agar, enough for 10 x 500 ml of medium

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$457.00
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RAPID'Listeria Supplement 2 #356-4746
RAPID'L.mono and RAPID'Listeria spp. Supplement 2

3564746
Pkg of 10, vials of supplement 2, enough for 10 x 500 ml of medium

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$160.00
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Fraser 1/2 Broth #3555797
Fraser 1/2 Broth, ready to use

3555797
Pkg of 6, 225 ml bottles

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$50.00
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Fraser Broth Base #356-4604
Fraser Broth Base, dehydrated

3564604
Pkg of 1, 500 g of dehydrated broth base

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$150.00
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Fraser 1/2 Supplement #356-4616
Fraser 1/2 Strength Supplement

3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium

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$257.00
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iQ-Check Listeria spp. kit #357-8113
iQ-Check® Listeria spp. PCR Detection Kit

3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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iQ-Check Listeria monocytogenes II kit #357-8124
iQ-Check® Listeria monocytogenes II PCR Detection Kit

3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
PF_264 Gélose RAPID’Listeria spp. Information Produit (Tous pays, sauf USA and Canada) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
TS_RListeria_spp RAPID'Listeria spp. Agar Technical Sheet, v. 2 (U.S. and Canada) Click to download
TS_RListeria_en RAPID’Listeria spp. Agar Technical Sheet, V2_05/2011 (All countries except U.S. and Canada) Click to download
FT_Rlisteria_fr RAPID’Listeria spp./Gélose Fiche Technique, V2_05/2011 (Tous pays, sauf USA and Canada) Click to download