RAPID'L.mono Medium

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Overview

Key Benefits
  • Fast
    • 24 hr incubation only for L. monocytogenes detection and enumeration
    • Easy-to-read chromogenic reaction
  • Simple and Affordable
    • Economical (1 broth, 1 plate)
    • Easy confirmation
  • Easy to Read
    • Blue colonies are L. monocytogenes
    • Blue-green colonies with distinct yellow halos are L. ivanovii
    • White colonies are other Listeria species
Protocol
Validation

Validated and Adapted for Food Detection

AFNOR AFNOR
BRD 07/04-09/98
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com
BRD 07/05-09/01
ALTERNATIVE ANALYTICAL METHODS FOR AGRIBUSINESS
Certified by AFNOR Certification
www.afnor-validation.com

The RAPID’L.mono method is validated as follows:

  • AFNOR Certification according to the ISO 16140 requirements for the detection of Listeria monocytogenes
    and other Listeria (No. BRD: 07/04-09/98) and for the enumeration of Listeria monocytogenes (No. BRD: 07/05-09/01)
  • AOAC-RI for the detection of Listeria monocytogenes in 24 hr (No. 030406)
  • NordVal Validation Ref No# 022
  • FDA/BAM recommended


Intended Use

RAPID'L.mono Medium is a selective chromogenic medium for the direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples.

Key Benefits

  • Fast
    • L. monocytogenes detection 24 hr after enrichment*
    • Listeria spp. detection 24 and 48 hr after enrichment
    • L. monocytogenes enumeration in 24 hr without confirmation**
    • L. monocytogenes confirmation in 24 hr after using the iQ-Check® Listeria monocytogenes II Kit
  • Simple and Cost Effective
    • Only 1 broth, and 1 plate required for each sample
    • Easy confirmation
  • Easy-to-Read Chromogenic Reaction
    • L. monocytogenes (PIPLC+/xylose-) forms blue colonies
    • L. ivanovii (PIPLC+/xylose+) formsblue-green colonies with distinct yellow halos
    • Other Listeria species form white colonies
  • Multipurpose Chromogenic Medium
    • RAPID'L.mono is the only chromogenic method to be validated for L. monocytogenes and Listeria spp. detection on the same plate
    • Can also be used for the enumeration or confirmation of L. monocytogenes
  • Validation
    • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

* Within the scope of ISO 16140 NordVal validation, no confirmation is required.

** Within the scope of the AFNOR validation, confirmation is not required when the presence of L. monocytogenes has been confirmed at the detection stage.

Principle

Reading and identification of L. monocytogenes are easy due to the unique chromogenic principle (PI-PLC activity) of RAPID'L.mono medium and the fermentation of a sugar (xylose)

  • L. monocytogenes (PIPLC+/xylose-) forms blue colonies
  • L. ivanovii (PIPLC+/xylose+) forms blue colonies with a yellow halo
  • Other Listeria spp. (PIPLC-/xylose+ or -) form white colonies with or without a yellow halo

RAPID'L.mono medium is very specific and inhibits the growth of other bacteria and yeasts.

The rhamnose test (< 6hr) and spot inoculation on Agar Listeria (AL agar) have been validated for confirmation of colonies grown on RAPID'L.mono medium.

Other Applications of RAPID'L.mono Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 11290 reference method)
  • To confirm positive results obtained using the iQ-Check Listeria monocytogenes II PCR Detection Kit
  • To enumerate L. monocytogenes in 24 hr without confirmation

RAPID'L.mono Chromogenic Assay

Enzymatic Activity
L.
monocytogenes

L.
ivanovii

L.
innocua

L.
welshimeri
PI-PLC detection + + - -
Xylose fermentation - + - +
     

Ordering Information

RAPID'L.mono Medium is offered as ready-to-use plates (3563964) or as a dehydrated agar base (3564293).

When using the dehydrated broth base, RAPID'L.mono Supplement 1 (3564294) and RAPID'L.mono Supplement 2 (3564746) need to be added to the molten agar prior to pouring plates.

For convenience, RAPID'L.mono Medium is also available as a kit (3555294) comprising 190 ml of medium and one vial of each supplement.

Assay type Chromogenic media
Organism Listeria monocytogenes, Listeria spp.
x

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RAPID'L.mono Agar #355-5294
RAPID'L.mono Agar Medium with Supplements

3555294
Pkg of 1, kit contains 190 ml of medium, 1 vial each of supplement 1 (3564294) and supplement 2 (3564746)

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$48.00
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RAPID'L.mono Agar #356-3694
RAPID'L.mono Agar Plates

3563694
Pkg of 20, 90 mm agar plates

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$82.00
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RAPID´L.mono Agar, dehydrated

3564293
Pkg of 1, 500 g of dehydrated agar base, enough for 7.4 L of medium

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$692.00
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RAPID´L.mono Supplement 1

3564294
Pkg of 10, vials of supplement 1 for 'RAPID´L.mono Agar, enough for 10 x 500 ml of medium

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$341.00
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RAPID'Listeria Supplement 2 #356-4746
RAPID'L.mono and RAPID'Listeria spp. Supplement 2

3564746
Pkg of 10, vials of supplement 2, enough for 10 x 500 ml of medium

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$160.00
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Fraser 1/2 Broth #3555797
Fraser 1/2 Broth, ready to use

3555797
Pkg of 6, 225 ml bottles

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$50.00
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Fraser Broth Base #356-4604
Fraser Broth Base, dehydrated

3564604
Pkg of 1, 500 g of dehydrated broth base

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$99.00
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Fraser 1/2 Supplement #356-4616
Fraser 1/2 Strength Supplement

3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium

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$113.00
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Buffered Peptone Water Broth, ready to use

3554179
Pkg of 6, 225 ml bottles

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$32.00
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Buffered Peptone Water Broth, dehydrated

3564684
Pkg of 1, 500 g

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$72.00
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iQ-Check Listeria spp. kit #357-8113
iQ-Check® Listeria spp. PCR Detection Kit

3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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iQ-Check Listeria monocytogenes II kit #357-8124
iQ-Check® Listeria monocytogenes II PCR Detection Kit

3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Number Description Options
FSD_16288 RAPID’Chromogenic Media Brochure, Rev L Click to download
PF_158 RAPID’Chromogenic Methoden für die Lebensmittel- und Umweltanalytik, Rev C (Deutsch) Click to download
PF_159 Métodos RAPID’Chromogenic para Análisis de Alimentos y Medioambiente, Rev C (Español) Click to download
PF_160 Méthodes RAPID’Chromogenic pour les Tests Alimentaires et Environnementaux, Rev C (Français) Click to download
PF_161 Metodi RAPID’Chromogenic per Analisi Alimentari e Ambientali, Rev C (Italiano) Click to download
PF_162 Métodos RAPID’Cromogénicos para Testes Alimentares e Ambientais, Rev C (Português) Click to download
FSD_17933 Food Safety and Quality Diagnostics Booklet, Rev I Click to download
TS_RLMono RAPID'L.mono Agar Technical Sheet v. 12 (U.S. and Canada) Click to download
TS_RLMono_en RAPID'L.mono Agar Technical Sheet v. 12 (All Countries except U.S. and Canada) Click to download
FT_RLMono_fr Gélose RAPID’L.mono Fiche Technique (Tous pays, sauf USA & Canada) Click to download