RAPID'E.coli O157:H7 Medium

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Overview


Key Benefits

KEY BENEFITS

  • Specific to E.coli O157 serotype H7
  • Only 1 plate required per sample
  • Complete results in 48 hr

Protocol

Validation

Validated and Adapted for Food Detection

The RAPID’E.coli O157:H7 method is certified by the AFNOR certification according to the ISO 16140 requirements for the detection of E. coli O157:H7 (No. BRD 07/14–09/07) and AOAC-RI validated (No. 060701).

Intended Use
RAPID’E.coli ™ O157:H7 medium is a selective chromogenic agar for the detection, isolation, and presumptive identification of Escherichia coli O157:H7 in human food products and environmental samples.

Simple Protocol

RAPID’E.coli O157:H7 medium provides laboratories with a rapid and economical solution for E.coli O157:H7 detection, with one plate required per sample. Easy confirmation using latex is also validated from isolated colonies on RAPID’E.coli O157:H7 agar.

Other Protocols

RAPID’E.coli O157:H7 medium can also be used

  • As a second selective medium in different standardized protocols (for example, ISO 16654 reference method)
  • To confirm positive PCR results of the iQ-Check® E.coli O157:H7 kit

Easy to Read

RAPID’E.coli O157:H7 medium permits detection of E. coli O157 serotype H7 using specific chromogenic substrates and biochemical activities. Typical E. coli O157:H7 (sorbitol and β-Glucuronidase) present characteristic convex 1–2 mm diameter dark blue to black colonies. It is possible to detect atypical β-Glucuronidase+ E. coli O157:H7. These strains have characteristic dark blue to black colonies.

Atypical sorbitol + E. coli O157:H7 are also detected. These colonies are blue to turquoise blue.

The selectivity of the medium is improved by adding selective reagents: novobiocin and potassium tellurite.

RAPID’E.coli O157:H7 medium is very specific. Most other bacteria and yeasts are inhibited or present a different color.

Assay type Chromogenic media
Organism E. coli O157:H7
RAPID'E. coli O157:H7 #356-4748
RAPID'E. coli O157:H7

356-4748
dehydrated, 100 g

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STEC Enrichment Broth
STEC Enrichment Broth

356-4001
500 g

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mTSB + Novobiocin Broth ready to use #355-5426
mTSB + Novobiocin Broth ready to use

355-5426
(containing novobiocin), 225 ml x 6 bottles

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mTSB Broth dehydrated (base) #356-4426
mTSB Broth dehydrated (base)

356-4426
500 g

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Novobiocin #356-4610
Novobiocin

356-4610
1 g

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Number Description Options
FSD_16234 Rapid E.coli O157 H7 Click to download
FSD_16288 RAPID’Chromogenic Methods for Food and Environmental Testing Brochure, Rev H Click to download
TS_REC_O157:H7 RAPID'E.coli O157:H7 Agar Technical Sheet, v.2 (US and Canada Only) Click to download