QX200™ Droplet Digital™ PCR System

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QX200 Droplet Digital PCR System

The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications..

Benefits of the QX200 Droplet Digital PCR System

  • Most precise and sensitive digital PCR solution for a wide variety of applications
  • Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays
  • Flexible assay setup — scalable for high sensitivity or high throughput
  • Simple and easy-to-use workflow with 96-sample throughput
  • Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors
  • Convenient assay design — standard curves are not required

See performance data for the QX200 Droplet Digital PCR System. ddPCR instrument control and analysis are performed by QuantaSoft™ Software.

Watch our Success Stories videos to learn more about the applications of the Droplet Digital System in diverse research fields.

View our Droplet Digital PCR reference library with links to scientific journal articles, conference posters, BioRadiations articles and Bio-Rad technical reports.

Applications of the QX200 Droplet Digital PCR System

  • Cancer biomarker studies and copy number variation
    Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. PrimePCR™ ddPCR Assays are now available for mutation and copy number detection. These are predesigned, wet-lab validated assays.

  • Pathogen detection
    Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.

  • Next generation sequencing
    Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.

  • Gene expression analysis
    Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.

  • Environmental monitoring
    Test a wide variety of environmental samples like soil and water using the QX200 System.

  • Food testing
    Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.
Droplet Digital PCR applications guide Download the Droplet Digital PCR Applications Guide for in-depth information about setting up experiments for applications including:
  • Copy number variation analysis
  • Rare mutation and sequence detection
  • Gene expression
  • Next-generation sequencing library analysis

Droplet Digital PCR Technology and Workflow

The QX200 Droplet Digital PCR System consists of two instruments, the QX200 Droplet Generator and the QX200 Droplet Reader, and their associated consumables.

The QX200 Droplet Generator is used to partition ddPCR reaction mix into thousands of nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 Droplet Reader. Droplets are read well by well as they are streamed single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.

QX200 Droplet Digital PCR system workflow

Additional Instruments

The QX200 Droplet Digital PCR System requires the use of the C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module and the PX1™ PCR Plate Sealer.

Required Accessories

ddPCR Reagents for Hydrolysis Probe-Based Applications

ddPCR Reagents for EvaGreen Applications

More Information

Explore the features of the QX200 System in more depth in the QX200™ Droplet Digital™ PCR System Brochure (bulletin 6311) or contact your Bio-Rad sales representative for more information.

Starting sample size, µl 20
QX200 droplet generator capacity 1–8 samples/cartridge
Droplets per 20 µl sample 20,000
QX200 droplet reader capacity 1–96 samples
Sample illumination Light-emitting diodes
Sample detection Multi-pixel photon counter
Detection channels FAM (EvaGreen), HEX (VIC)
Linear dynamic range 5 orders of magnitude
Precision ±10%
Droplets per 96-well plate, million ~1.5
QX200 droplet generator dimensions (W x D x H) 28 x 36 x 13 cm (11 x 14 x 5")
QX200 droplet reader dimensions (W x D x H) 66 x 52 x 29 cm (26 x 20 x 11")
QX200™ Droplet Digital™ PCR System

186-4001
Droplet Digital™ PCR System, includes droplet generator, droplet reader, laptop computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications

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QX200™ Droplet Generator

186-4002
Droplet generator for EvaGreen or probe-based digital PCR applications, for use with QX200™ Droplet Digital™ PCR System

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QX200™ Droplet Reader

186-4003
Droplet reader for EvaGreen or probe-based digital PCR applications, for use with the QX200™ Droplet Digital™ System

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PX1™ PCR Plate Sealer

181-4000
PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

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C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module

185-1197
Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96–deep well reaction module, USB flash drive

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QuantaSoft™ Software, Regulatory Edition

186-4011
QuantaSoft Software, version 1.7, Regulatory Edition, for use with all QX100™ and QX200™ Droplet Digital™ PCR (ddPCR™) Systems

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Number Description Options
6311 QX200 Droplet Digital PCR System Brochure, Rev B Click to download
10031907 Instruction Manual, QX200 Droplet Generator, Rev C Click to download
10031906 Instruction Manual, QX200 Droplet Reader and QuantaSoft Software, Rev D Click to download
10047467 Instruction Manual, QuantaSoft™ Version 1.7 Regulatory Edition Software, Rev A Click to download
10023997 Instruction Manual, PX1 PCR Plate Sealer, Rev A Click to download
10021377 Instruction Manual, C1000 Touch Thermal Cycler, Rev B Click to download
6512 PrimePCR Assays for Droplet Digital PCR Brochure, Rev A Click to download
6338 Droplet Digital™ PCR (ddPCR™) Supermixes Flier, Rev A Click to download
6473 QX200 ddPCR EvaGreen Supermix Flier, Rev A Click to download
6250 One-Step RT-ddPCR Kit for Probes Flier, Rev A Click to download
6402 ddPCR Library Quantification Kit for Illumina TruSeq Flier, Rev B Click to download
6490 ddPCR Library Quantification Kit for Ion Torrent Flier, Rev A Click to download
6407 Droplet Digital PCR Applications Guide, Rev A Click to download
6450 Droplet Digital™ PCR Publications List, Rev J Click to download
6343 QX100 Droplet Digital PCR System for Accurate Quantification of Illumina TruSeq Libraries, Rev B Click to download
6495 Bio-Rad’s ddPCR™ Library Quantification Kit for Ion Torrent Enables Accurate Library Quantification and Excellent Balancing of Pooled Libraries, Rev A Click to download
6554 Droplet Digital™ PCR: Detection of DNA Methylation Application Note, Rev A Click to download [ Add to Cart (Free) ]
6541 Droplet Digital™ PCR: TRAPing Telomerase Activity Using Droplet Digital PCR Application Note, Rev A Click to download
6451 Droplet Digital PCR: Guidelines for Multiplexing Using Bio-Rad’s QX100 Droplet Digital PCR System Application Note, Rev A Click to download
6497 Droplet Digital PCR: Linking Microbial Physiology and Phylogeny Using Bio-Rad’s QX100 Droplet Digital PCR System Application Note, Rev A Click to download
6475 Droplet Digital PCR: High-Resolution Copy Number Variation Analysis Application Note, Rev A Click to download
6424 Droplet Digital™ PCR: Ultra-Sensitive Rare Event Detection Using Bio-Rad’s QX100™ Droplet Digital PCR System Application Note, Rev B Click to download
6423 Droplet Digital PCR: Ultra-Sensitive Validation Technology for RNA-Seq Application Note, Rev A Click to download [ Add to Cart (Free) ]
6319 Third Generation PCR: A Novel Way to Accurately Measure Copy Number Variations in Human Genome [Harvard laboratory interview]. BioRadiations, Featured Stories, February 9, 2012 Click to download
6318 Droplet Digital PCR Opens New Perspectives in HIV Research [Sangamo BioSciences, Inc. laboratory interview]. BioRadiations, Solutions, June 15, 2012 Click to download
6317 Detection of rare mutations in blood samples by Droplet Digital PCR (ddPCR). (Poster), Rev A Click to download
6316 Droplet Digital RT-ddPCR: Ultra-high sensitivity validation technology for RNA-Seq. (Poster), Rev A Click to download
6315 Droplet Digital PCR: 3rd generation PCR provides improved detection, quantification, identification, and characterization of microbial targets. (Poster), Rev A Click to download
6314 Droplet Digital PCR detection of HER2 expression in FFPE breast tissue samples (Poster), Rev A Click to download
6277 Probing Copy Number Variations Using Bio-Rad's QX100 Droplet Digital PCR System, Rev A Click to download [ Add to Cart (Free) ]
6578 Droplet Digital™ PCR: Multiplex Detection of KRAS Mutations in Formalin-Fixed, Paraffin-Embedded Colorectal Cancer Samples Application Note, Rev A Click to download [ Add to Cart (Free) ]
6679 Droplet Digital™ PCR: Multiplex Screening of KRAS Mutations in Cell-Free DNA Colorectal Cancer Samples Application Note, Rev A Click to download
6681 Discriminating Copy Number Variation from Heterogeneous Samples Using the S3™ Cell Sorter and the QX200™ Droplet Digital™ PCR System Application Note, Rev A Click to download
10026868 ddPCR™ Supermix for Probes (No dUTP) Product Insert, Rev D Click to download
10026235 ddPCR™ Supermix for Probes Product Insert, Rev C Click to download
10028376 QX200™ ddPCR™ EvaGreen Supermix Product Insert, Rev C Click to download
10028782 One-Step RT-ddPCR Kit for Probes Product Insert, Rev B Click to download
10031986 ddPCR Library Quantification Kit for Illumina TruSeq Product Insert, Rev C Click to download
10033630 ddPCR Library Quantification Kit for Ion Torrent Product Insert, Rev B Click to download
10040718 Droplet Generation Oil for Probes Product Insert, Rev B Click to download
10040717 QX200 Droplet Generation Oil for EvaGreen Product Insert, Rev B Click to download
6628 Rare Mutation Detection Best Practices Guidelines Click to download