Pyridinium Crosslinks by HPLC (Reversed Phase)

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Pyridinium Crosslinks by HPLC (Reversed Phase)

Crosslinks by HPLC, available for both manual and automated methods (on Gilson ASPEC™ systems), is used for the quantitative determination of pyridinoline (PYD) and deoxypyridinoline (DPD) in urine.

The pyridinium derivatives, pyridinoline (PYD) and deoxypyridinoline (DPD), are considered the most specific markers of bone resorption. Measurement of these biochemical markers of bone metabolism provides information concerning the dynamics of bone transformation and the related loss of bone mass and allows control of progression or therapy of osteoporosis, for example. This test features a unique internal standard with calibration traceable to CDC reference material. Easy specimen preparation eliminates interfering substances, and no special dietary restrictions are required.

The automated application, which is designed for use with Gilson ASPEC™ XL or GX-271, offers high specimen through-put and reduced hands-on time, with an overnight processing option.

Bio-Rad has developed automated protocols for many of our HPLC kits with Gilson ASPEC™ XL or GX-271. Each protocol is validated for complete confidence in the results. These methods help reduce laboratory costs by dramatically decreasing labor hours. Contact your local Bio-Rad representative for more information about Gilson ASPEC™ methods.

Test characteristics:

  • Principle: Isocratic HPLC method (Reversed Phase)
  • Duration of chromatography: 16 minutes
  • Sample volume: 150 µL urine
  • Flow rate: 0.7 mL/min
  • Fluorescence Detection: exitation: 295 nm, emission: 400 nm

 

Pyridinium-Crosslinks by HPLC Reagent Kit
Pyridinium-Crosslinks by HPLC Reagent Kit

195-6576
Reagent kit for analysis of pyridinoline (PYD) and deoxypyridinoline (DPD) in urine by HPLC (100 tests)

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Reagent Set
Reagent Set

195-6576R
Reagents for Pyridinium-Crosslinks by HPLC (1 unit)

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Pyridinium-Crosslinks by HPLC Reagent Kit
Pyridinium-Crosslinks by HPLC Reagent Kit

195-6596
Reagent kit for analysis of pyridinoline (PYD) and deoxypyridinoline (DPD) in urine by HPLC (100 tests)

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Reagent Set
Reagent Set

195-6596R
Reagents for Pyridinium-Crosslinks by HPLC Reagent Kit (1 unit)

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Pyridinium Crosslinks by HPLC Reagent Kit, Automated Method
Pyridinium Crosslinks by HPLC Reagent Kit, Automated Method

195-5930
Reagents for automated analysis of pyridinoline (PYD) and deoxypyridinoline (DPD) in urine by HPLC on Gilson ASPEC systems (300 tests)

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Reagent Set
Reagent Set

195-5930R
Reagents for Pyridinium-Crosslinks by HPLC Reagent Kit, automated (1 unit)

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Analytical Cartridge for Pyridinium Crosslinks by HPLC Reagent Kit
Analytical Cartridge for Pyridinium Crosslinks by HPLC Reagent Kit

195-5929
One separation cartridge and 2 microguard cartridges for Pyridinium Crosslinks by HPLC assay (1 package; 500 injections)

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Analytical Cartridge Holder

195-5925
Holder for analytical cartridge used for HPLC assays (1 unit)

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Micro-Guard Cartridge Holder

195-5030
1 holder for high performance liquid chromatography guard cartridge

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Valve-device for Gilson ASPEC, Automated Method
Valve-device for Gilson ASPEC, Automated Method

195-9530
Valve-device for HPLC on Gilson ASPEC, automated method (1 unit)

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Application Disk for ASPEC V 2.0, Automated Method
Application Disk for ASPEC V 2.0, Automated Method

195-5954
Software disk for automation of HPLC assays on Gilson ASPEC systems (1 disk)

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Disposable Columns for Pyridinium Crosslinks by HPLC Reagent Kit
Disposable Columns for Pyridinium Crosslinks by HPLC Reagent Kit

195-6577
Disposable columns for use with Pyridinium Crosslinks by HPLC (100 columns)

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Disposable Columns for Pyridinium Crosslinks by HPLC Reagent Kit, Automated Method
Disposable Columns for Pyridinium Crosslinks by HPLC Reagent Kit, Automated Method

195-6575
Disposable columns for use with Pyridinium Crosslinks by HPLC, automated method (100 columns)

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Test Mix for Pyridinium Crosslinks by HPLC Reagent Kit
Test Mix for Pyridinium Crosslinks by HPLC Reagent Kit

195-5915
Test mixture to verify performance of Pyridinium Crosslinks by HPLC (2.5 mL)

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Urine Calibrator Set (mono-level) for Pyridinium Crosslinks by HPLC Reagent Kit
Urine Calibrator Set (mono-level) for Pyridinium Crosslinks by HPLC Reagent Kit

195-6579
Mono-level urine calibrator set for Pyridinium Crosslinks by HPLC (2 x 3 mL)

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Mobile Phase for Pyridinium Crosslinks by HPLC Reagent Kit
Mobile Phase for Pyridinium Crosslinks by HPLC Reagent Kit

195-6570
Mobile phase for Pyridinium Crosslinks by HPLC assay (1700 mL)

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Micro-Guard Refill Cartridges

195-6003
Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections

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 Pyridinium Crosslinks by HPLC Control Set (bi-level)
Pyridinium Crosslinks by HPLC Control Set (bi-level)

195-5959
Bi-level, urine-based controls for pyridinoline and deoxypyridinoline (2 x 2 x 5 mL)

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Hydrolysis glass tubes
Hydrolysis glass tubes

195-9509
Glass tube set for precolumn sample derivatization prior to assay of hydroxyproline by HPLC (100 tubes)

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