Crosslinks by HPLC, available for both manual and automated methods (on Gilson ASPEC™ systems), is used for the quantitative determination of pyridinoline (PYD) and deoxypyridinoline (DPD) in urine.
The pyridinium derivatives, pyridinoline (PYD) and deoxypyridinoline (DPD), are considered the most specific markers of bone resorption. Measurement of these biochemical markers of bone metabolism provides information concerning the dynamics of bone transformation and the related loss of bone mass and allows control of progression or therapy of osteoporosis, for example. This test features a unique internal standard with calibration traceable to CDC reference material. Easy specimen preparation eliminates interfering substances, and no special dietary restrictions are required.
The automated application, which is designed for use with Gilson ASPEC™ XL or GX-271, offers high specimen through-put and reduced hands-on time, with an overnight processing option.
Bio-Rad has developed automated protocols for many of our HPLC kits with Gilson ASPEC™ XL or GX-271. Each protocol is validated for complete confidence in the results. These methods help reduce laboratory costs by dramatically decreasing labor hours. Contact your local Bio-Rad representative for more information about Gilson ASPEC™ methods.
- Principle: Isocratic HPLC method (Reversed Phase)
- Duration of chromatography: 16 minutes
- Sample volume: 150 µL urine
- Flow rate: 0.7 mL/min
- Fluorescence Detection: exitation: 295 nm, emission: 400 nm