ProteOn Manager™ software guides the user through protein interaction studies, allowing sophisticated experiments to be completed with ease. A single software interface controls instrument setup, experiment design, data collection, and analysis.
The instrument control panel includes maintenance wizards that direct the user to weekly and monthly cleaning protocols to keep the instrument running at optimum performance. Additionally, the instrument control panel logs all instrument functions in a searchable and exportable database.
The protocol editor offers a variety of formats for creating a protocol easily. Users may design templates, create custom protocols, or use the automatic protocol option depending on their workflow. Samples may be imported or manually entered using copy and paste commands or using group functions for iterative tasks. Pull-down menus help navigate samples into flexible locations. Once samples are entered, data types and concentration can be selected to aid in downstream analysis. Graphics are shown to help visualize orientation of sample injection flow.
Sample layouts are visually displayed in several ways. Position is indicated by color-coordinated graphics that represent the sample position in the protocol. Pull-down menus in the sample panels allow the user to easily switch sample locations while retaining all sample information.
The software interface allows users to visualize real-time interactions on all 36 spots. Raw data collected from the instrument can be processed and analyzed with ProteOn Manager software. Data at any stage — raw, processed, or analyzed — can be exported. Sensorgrams may be exported in either a metafile or data format for use in presentations and publications.
Data processing is easy with the click of a single button with undo functions to illustrate the effects of different processing parameters to a data set. Users can instantly adjust the baseline and injection alignment parameters and remove artifacts using the Autoprocess button. Data processing functions can also be performed manually.
The software supports a comprehensive set of referencing options, including interspot or channel referencing, real-time injection reference, double referencing, and excluded volume correction for solvents like DMSO. Referencing is selected and applied with the click of a button.
Data analysis is made simple through the use of intuitive software wizards. Wizards allow step-by-step data collection for kinetic, equilibrium, and concentration analysis. Association, dissociation, and affinity constants are reported in the data table and can be easily exported. Each analysis mode allows the user to define analysis parameters, perform calculations, and review and print results. Analysis with molecular weight normalization is available. Seven kinetic fit models are supported, including Langmuir 1:1, Langmuir with drift, Langmuir mass transfer, bivalent analyte, heterogeneous analyte, heterogeneous ligand, and conformational change, as well as separate off-rate only analysis.
ProteOn Manager Software, Security Edition
New compliance features enable the user to turn on security features that comply with U.S. FDA 21 CFR Part 11 regulations. Features of the Security Edition software include audit trails, electronic signatures, data validation, user logins and permissions, and closed-system security. Signatures can be verified, ensuring data consistency within the instrument.