Profinity IMAC Resins

Profinity IMAC Resins

Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins.

Read More
Ordering Information

Overview

Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins. Immobilized metal affinity chromatography (IMAC) is a powerful purification technique that relies on the affinity that His has for immobilized transition metals. Profinity IMAC resin, based on UNOsphere beads, contains iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.

Profinity IMAC resin offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions and is suitable for purification using liquid chromatographic instrumentation, gravity-flow columns, or spin columns.

The resin is available in two forms: uncharged and precharged with Ni2+. The uncharged form can be charged with the metal ion of your choice for even greater purification flexibility.

Features of Profinity IMAC resins include:

  • Optimal ligand density for higher purity of target protein
  • Superb mechanical strength
  • Excellent pressure-flow properties for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and re-equilibration
  • Stable from pH 1 to 14
  • Compatible with denaturing agents, detergents, and reducing agents

For more information, request bulletin 5456.

Larger volumes and special packaging for industrial applications are available upon request.

Ordering

items

Use the filters below to refine results!

Image
156-0121_tn.jpg
1560121

Pkg of 1, 10 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

Image
156-0123_tn.jpg
1560123

Pkg of 1, 50 ml, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

Image
156-0127_tn.jpg
1560127

Pkg of 1, 1 L, support with uncharged iminodiacetic acid (IDA) for immobilized metal affinity chromatography

Image
156-0131_tn.jpg
1560131

Pkg of 1, 10 ml, ready-to-use support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

Image
156-0133_tn.jpg
1560133

Pkg of 1, 25 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

Image
156-0135_tn.jpg
1560135

Pkg of 1, 100 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

Image
156-0137_tn.jpg
1560137

Pkg of 1, 500 ml, support with nickel-charged iminodiacetic acid (NIDA) for immobilized metal affinity chromatography

Image
EconoFit_Columns-5x1ml-Sku_TN.png
12009298

Pkg of 5, 1 ml, prepacked Ni-charged column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

Image
EconoFit_Columns-1x5ml-Sku_TN.png
12009299

Pkg of 1, 5 ml, prepacked Ni-charged column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

Image
EconoFit_Columns-5x5ml-Sku_TN.png
12009300

Pkg of 5, 5 ml, prepacked Ni-charged column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

Documents

Number Description Options
5456
Profinity™ IMAC Resin: An Optimal Support for Histidine-Tagged Protein Purification, Rev B
3193
Profinity IMAC Resin Product Information Sheet
5283
Profinity IMAC Ni-Charged Resin Flier, Rev A
5251
Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins, Rev A
5501
Rapid, Efficient Purification and Evaluation of His-Tagged Proteins, Rev A
5444
Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet, Rev A
5514
Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A
10001677
Instruction Manual, Profinity IMAC Resins, Rev B
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
10000105043
Instruction Manual, EconoFit Profinity IMAC Columns