Profinity IMAC resin is an affinity chromatography support for the purification of recombinant histidine (His)-tagged proteins. Immobilized metal affinity chromatography (IMAC) is a powerful purification technique that relies on the affinity that His has for immobilized transition metals. Profinity IMAC resin, based on UNOsphere beads, contains iminodiacetic acid (IDA) as the chelating ligand for di- or trivalent metal ions. Its chemical structure allows highly selective binding of recombinant His-tagged proteins when charged with Ni2+ or other transition metals, such as Zn2+ or Cu2+.
Profinity IMAC offers high capacity at high flow rates. It can be used under either nondenaturing or denaturing conditions, and is suitable for purification using liquid chromatographic instrumentation, gravity-flow columns, or spin columns.
The resin is available in two forms: uncharged and precharged with Ni2+. The uncharged form can be charged with the metal ion of your choice for even greater purification flexibility.
Features of Profinity IMAC resins include:
- Optimal ligand density for higher purity of target protein
- Superb mechanical strength
- Excellent pressure-flow properties for high maximum operating pressures and high flow rates, which allow rapid purification as well as rapid column cleaning and re-equilibration
- Stable from pH 1 to 14
- Compatible with denaturing agents, detergents, and reducing agents