Profinia™ Protein Purification Instrument

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Profinia™ Protein Purification System Demo

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Profinia Application Overview
Method Available Kits, Buffers and Cartridges Reference
(Bulletin
Number)
Native IMAC Profinia native purification kit, Profinia native buffer kit, Profinia native IMAC starter kit, Bio-Scale Mini Profinity IMAC cartridges 5444, 5574, 5548, 5514
Native IMAC plus desalting Profinia native purification kit, Profinia native buffer kit, Profinia native IMAC starter kit, Profinia desalting purification kit, Profinia desalting buffer kit, Bio-Scale Mini Profinity IMAC cartridges, and Bio-Scale Mini Bio-Gel P-6 desalting cartridges 5444, 5574, 5548, 5514
Denaturing IMAC Profinia denaturing IMAC purification kit, Profinia denaturing buffer kit, Bio-Scale Profinity IMAC cartridges 5574, 5547, 5514
GST Profinia GST purification kit, Profinia GST buffer kit, Bio-Scale Mini Profinity GST cartridges 5574, 5548, 5514
GST plus desalting Profinia GST purification kit, Profinia GST buffer kit, Profinia desalting purification kit, Profinia desalting buffer kit, Bio-Scale Mini Profinity GST cartridges, and Bio-Scale Mini Bio-Gel desalting cartridges 5574, 5548, 5513
Desalting Profinia desalting purification kit, Profinia desalting buffer kit, and Bio-Scale Mini Bio-Gel P-6 desalting cartridges 5539, 5548
Protein A and G Profinia desalting purification kit, Profinia desalting buffer kit, and Bio-Scale Mini Affi-Prep protein A cartridges 5701, 5726, 5712
Protein A and G plus desalting Profinia desalting purification kit, Profinia desalting buffer kit, Bio-Scale Mini Affi-Prep protein A cartridges, and Bio-Scale Mini Bio-Gel P-6 desalting cartridges 5701, 5726, 5712
Profinity eXact purification Profinia desalting purification kit, Profinia desalting buffer kit, and Bio-Scale Mini Profinity eXact cartridges 5725, 5742
Maltose-binding protein (MBP) Affinity method (in Program methods mode) 5741
StrepTactin Affinity method (in Program methods mode) 5744

The Profinia protein purification instrument is compact and easy to use. Each compartment of the instrument is clearly labeled for fast setup. The buffer compartment allows simple installation of buffers — numbered positions on the instrument match run diagrams and prepackaged reagent labels. The sample compartment is capable of running two samples in series. Both compartments have sipper tubes for the uptake of buffers and samples. The cartridge compartment offers two-step purification capability and uses luer fittings to simplify cartridge connection. Waste collection and diluent bottles are in view and accessible on the sides of the instrument. The fraction collection and sample compartments allow quick installation of standard collection tubes. A central compartment provides convenient storage for a stylus and access to a USB flash drive, which allows you to export run data.

The instrument has a large touch-screen user interface, where you can select options — by finger or stylus — to navigate from screen to screen. Intuitive, user-friendly help functions guide you through the setup and post-run functions of preprogrammed optimized purification methods. The methods and buffer sets include cleaning protocols that maintain the system and prepare the cartridges for future use, ensuring that the Profinia system stays in optimal running condition. This combination of features allows you to set up quickly, walk away, and come back to results in as little as 30 min.

  • Optimized methods, cartridges, and buffer kits eliminate time spent on method development, troubleshooting, and reagent preparation
  • Built-in reproducibility is achieved with automated system pumps, UV and conductivity detectors, and programmed cleaning methods
  • Histidine (His)-tagged, GST-tagged, affinity, and desalting/buffer exchange methods deliver purity and yield comparable to other techniques, and require only a fraction of the time to obtain protein ready for experiments
  • Automatic UV peak detection diverts eluted target protein from cartridges to a fraction collection tube for unattended operation
  • Easy-access fraction collection and sample compartments hold 15 or 50 ml conical collection tubes
  • Benchtop or coldroom operation allows you to control purification conditions; optional cooling accessory keeps samples and fractions cold for benchtop operation
  • Additional capabilities let you work with a pH monitor that runs in real time with Profinia software
Operating Limits  
Flow rate range 0.2–20 ml/min
Maximum backpressure 45 psi/3.4 bar
Operating temperature range 4–30ºC
Detection  
UV absorbance wavelength 280 nm
Conductivity monitor range 0–500 ms/cm
pH monitor (optional) pH range 1–14
Hardware Sample and fraction 15 or 50 ml conical tubes; can use larger external reservoirs connected by tubing
Collection containers Buffer selection rotary selector valves for up to 8 application-specific buffers
Pressure Sensor monitors system pressure
Solvent compatibility All commonly used chromatographic solvents
Data  
Output USB Flash drive or direct USB cable to PC connection
Reporting software (optional) Windows 2000 or XP; chromatographic data display, reporting, and analysis in real time or using stored run files
User interface Touch screen 115 x 86 mm; select method, sample, and cartridge options by stylus or finger
Optional accessories (sold separately) Sample and fraction cooling accessory; desalting sample loops; pH monitor; Profinia software
Physical Specifications  
Dimensions (W x D x H) 58 x 33 x 67 cm
Profinia Instrument With Accessory Kit and Native IMAC Starter Kit

620-1005
100–240 V, automated chromatography instrument, includes Profinia instrument accessory kit (#620-0410) and Profinia native IMAC starter kit (#620-0229)

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Profinia Instrument With Accessory Kit and GST Starter Kit

620-1006
100–240 V, automated chromatography instrument, includes Profinia instrument accessory kit (#620-0410) and Profinia GST starter kit (#620-0230)

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Profinia Protein Purification System With Native IMAC Starter Kit

620-1010
100–240 V, automated chromatography system, includes Profinia instrument and software, Profinia instrument accessory kit (#620-0410) and Profinia native IMAC starter kit (#620-0229)

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Profinia Protein Purification System With GST Starter Kit

620-1011
100–240 V, automated chromatography system, includes Profinia instrument and software, Profinia instrument accessory kit (#620-0410) and Profinia GST starter kit (#620-0230)

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Profinia Protein Purification System With Computer and Native IMAC Starter Kit

620-1015
100–240 V, automated chromatography system, includes Profinia instrument and software, computer, Profinia instrument accessory kit (#620-0410) and Profinia native IMAC starter kit (#620-0229)

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Profinia Protein Purification System With Computer and GST Starter Kit

620-1016
100–240 V, automated chromatography system, includes Profinia instrument and software, computer, Profinia instrument accessory kit (#620-0410) and Profinia GST starter kit (#620-0230)

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Number Description Options
10006036 Instruction Manual, Profinia Protein Purification System, Rev F Click to download
10015932 Profinia Protein Profinia Protein Purification System FAQs, Rev B Click to download
10015931 Understanding Chromatograms Generated by the Profinia Protein Purification System Reference Guide, Rev B Click to download [ Add to Cart (Free) ]
10006080 His Antibody Instruction Manual, Rev C Click to download
10006081 GST Antibody Instruction Manual, Rev C Click to download
5514 Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A Click to download [ Add to Cart (Free) ]
5513 Automated Purification of a GST-Tagged Protein With the Profinia Protein Purification System: Comparison to Manual Protein Purification Using Commercial Kits, Rev A Click to download [ Add to Cart (Free) ]
5539 Automated Desalting of Proteins With the Profinia Protein Purification System: Comparison to Manual Desalting by Dialysis, Rev A Click to download [ Add to Cart (Free) ]
5712 The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A Click to download [ Add to Cart (Free) ]
5726 Profinia Protein A and G methods Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5725 Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5741 Profinia Buffers for Maltose Binding Protein (MBP) Chromatography Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5742 Profinity eXact Purification Methods on the Profinia System, Rev. A Click to download [ Add to Cart (Free) ]
5744 Profinia Buffers for Strep-Tag Protein Chromatography Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5766 Purification of Functional Heterotrimeric G-Protein a-Subunits Using the Profinity eXact Fusion-Tag System on the Profinia Protein Purification System, Rev A Click to download [ Add to Cart (Free) ]
5573 The Profinia Protein Purification System: An Automated Solution for Purifying Affinity-Tagged Proteins, Rev A Click to download [ Add to Cart (Free) ]
5541 Profinia Protein Purification System Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5510 Profinia Protein Purification System Brochure, Rev A Click to download [ Add to Cart (Free) ]
5701 Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B Click to download [ Add to Cart (Free) ]
5870 Purification and On-Column Refolding of Polyhistidine-Tagged Staphylococcus aureus α- hemolysin Inclusion Bodies on the Profinia Protein Purification System, Rev A Click to download
5926 Modular Protein Purification, Rev A Click to download [ Add to Cart (Free) ]