Profinia Protein Purification System Buffers and Reagents

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Overview

Profinia Buffer Kits
Profinia Buffer Kits

Profinia buffer kits contain buffers and cleaning and storage reagents required for purification.

Profinia Control Lysate

The Profinia control lysate is a lyophilized, dual-tagged 51 kD target protein that is meant to eliminate concerns over variability in purification buffer solutions or in the purification matrix itself. The control lysate facilitates system setup and initial purification and is included in every Profinia IMAC and GST starter kit.

Urea and Glutathione Reagents

Powdered urea and glutathione reagents are available for use with denaturing IMAC and GST applications, respectively. The urea packs are ideal for solubilizing and denaturing proteins in E. coli extracts. The glutathione pack is essential for eluting GST fusion proteins from immobilized glutathione resins.

His and GST Antibodies

Histidine (His)-tagged and GST-tagged monoclonal antibodies are used to detect target protein expression of overexpressed 6x His and GST fusion proteins. They are supplied at a concentration of 1 mg/ml in phosphate buffered saline, pH 7.4 with 0.05% NaN3.

Individually packaged reagents are also available for use with the Profinia system. While designed specifically to work with the Profinia system, these reagents also have general applicability within all stages of a purification workflow, from sample preparation to protein detection.

Profinia Bottled Buffers
For added convenience, preformulated buffers are available for native IMAC, GST, and desalting purifications.

Profinia Control Lysate
The Profinia control lysate is a lyophilized, dual-tagged 51 kD target protein that is meant to eliminate concerns over variability in purification buffer solutions or in the purification matrix itself. The control lysate facilitates system setup and initial purification and is included in every Profinia native IMAC and GST starter kit.

Urea and Glutathione Reagents
Powdered urea and glutathione reagents are available for use with denaturing IMAC and GST applications, respectively. The urea packs are ideal for solubilizing and denaturing proteins in E. coli extracts. The glutathione pack is essential for eluting GST fusion proteins from immobilized glutathione resins.

His and GST Antibodies
Histidine (His)-tagged and GST-tagged monoclonal antibodies are used to detect target protein expression of overexpressed 6x His and GST fusion proteins. They are supplied at a concentration of 1 mg/ml in phosphate buffered saline, pH 7.4 with 0.05% NaN3.

Glutathione Pack

620-0202
1.23 g, powdered reagent, for denaturing GST applications on the Profinia protein purification system

List Price:   $57.00
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His Antibody

620-0203
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against histidine (His)-tagged proteins, for use with the Profinia protein purification system

List Price:   $387.00
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GST Antibody

620-0204
Pkg of 1 vial, 1 mg/ml, monoclonal antibody against GST-tagged proteins, for use with the Profinia protein purification system

List Price:   $420.00
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Native IMAC Lysis Buffer, 2x

620-0205
125 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 10 mM imidazole, pH 8.0, for use with His-tag protein purification

List Price:   $94.00
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Native IMAC Wash Buffer 1, 2x

620-0206
125 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 10 mM imidazole, pH 8.0, for use with His-tag protein purification

List Price:   $64.00
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Native IMAC Wash Buffer 2, 2x

620-0207
100 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 20 mM imidazole, pH 8.0, for use with His-tag protein purification

List Price:   $55.00
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Native IMAC Wash Buffer 1, 2x

620-0208
100 ml, preformulated concentrate, 600 mM KCl, 100 mM KH2PO4, 500 mM imidazole, pH 8.0, for use with His-tag protein purification

List Price:   $62.00
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GST Lysis buffer, 2x

620-0213
100 ml, preformulated concentrate, 40 mM glutathione, 200 mM Tris, 10 mM EDTA, pH 8.0, for use with GST-tag protein purification

List Price:   $123.00
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GST Wash buffer, 2x

620-0214
200 ml, preformulated concentrate, 300 mM NaCl, 20 mM Na2HP04, 10 mM EDTA, pH 7.4, for use with GST-tag protein purification

List Price:   $62.00
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GST Elution buffer, 2x

620-0215
100 ml, preformulated concentrate, 40 mM glutathione, 200 mM Tris, 10 mM EDTA, pH 8.0, for use with GST-tag protein purification

List Price:   $73.00
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Desalting Buffer, 5x

620-0216
200 ml, preformulated concentrate, contains NaCl, KCl, Na2HPO4, KH2PO4, pH 7.0, for use with protein desalting

List Price:   $73.00
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Cleaning Solution 1, 2x

620-0217
125 ml, preformulated concentrate, 1,000 mM NaCl, 100 mM Tris, pH 8.0, for use with cartridge cleaning

List Price:   $64.00
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Cleaning Solution 2, 4x

620-0218
125 ml, preformulated concentrate, 2,000 mM NaCl, 400 mM NaOAc, pH 4.5, for use with the cartridge cleaning

List Price:   $106.00
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Storage solution, 2x

620-0219
200 ml, preformulated concentrate, 4% C6H5CH2OH (benzyl alcohol), for use with cartridge storage

List Price:   $64.00
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His and GST Purification E. coli Control Lysate

620-0233
Lyophilized, dual-tagged 51 kD protein for system setup and initial purification, for use with the Profinia protein purification system

List Price:   $163.00
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Number Description Options
10006042 Instruction Manual, Profinia His Antibody, Rev A Click to download
10006040 Instruction Manual, Profinia GST Glutathione Pack, Rev B Click to download
10006043 Instruction Manual, Profinia GST Antibody, Rev A Click to download
10006041 Instruction Manual, Profinia Denaturing IMAC Urea Pack, Rev A Click to download
10006059 Instruction Manual, Profinia Control Lysate, Rev C Click to download
10006060 Instruction Manual, Profinia Bottled Buffers, Rev A Click to download