Prepacked Columns

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Overview

Bio-Spin Column; Micro Bio-Spin Column
Bio-Scale Mini Cartridges
Prepacked Column Selection Guide
Prepacked Column Selection Guide Bio-Spin® 6 Micro Bio-Spin® 6 Bio-Spin 30 Micro Bio-Spin 30
Packed support (special grade) Bio-Gel P-6 gel Bio-Gel P-6 gel Bio-Gel P-30 gel Bio-Gel P-30 gel
Equilibration buffer 10 mM Tris, pH 7.4 or SSC buffer (150 mM NaCl, 17.5 mM sodium citrate, pH 7.0)
Applications        
Desalting of oligonucleotides >20 bases
Labeling reactions: removal of unincorporated nucleotides >20 bases or bp from DNA
Buffer exchange (restriction fragments, PCR products, enzyme reactions, sequencing templates)
DNA sequencing reaction mixture cleanup
Riboprobe cleanup
Desalting of antibody, enzyme, and protein solutions
Purification of proteins of moleular weight >6,000
Purification of proteins of molecular weight >40,000
Prepacked Cartridge Selection Cartridge Support Type of Separation Chemical Form Protein Capacity/ml*
Protein and Plasmid Purification UNOsphere Q**
UNOsphere S**
UNOsphere Q**
UNOsphere S**
Strong anion exchange
Strong anion exchange
–N+(CH3)3
–SO-3
180 mg BSA
60 mg human IgG
Fusion Tagged Protein Purification IMAC** Profinity IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
His-tagged protein

* Protein capacity based on bulk support; check individual instruction
manuals for run conditions and specifications.
** Available in Bio-Scale mini format.

One of the most common methods for scientific discovery is the purification of biomolecules via column chromatography. Chromatography is a separation method in which components of a mixture are distributed between two phases: a mobile phase and a stationary phase. Bio-Rad offers numerous prepacked chromatography columns in multiple sizes for purification and characterization of proteins, nucleic acids, and peptides.

Bio-Spin and Micro Bio-Spin Columns
Prepacked Bio-Spin and Micro Bio-Spin columns clean up protein and DNA samples quickly. These columns are filled with specially sized Bio-Gel P gels and are shipped fully hydrated and ready to use. These products effectively clean up and remove salts, nucleotides, and small molecules from DNA, RNA, and protein samples in just 10 min. Prepacked columns offer the convenience of media provided in a format ready to use for common applications.

Bio-Scale Mini Cartridges
Prepacked Bio-Scale Mini cartridges are convenient, disposable, and supplied ready for use in protein separation. The cartridges are prepacked with Bio-Rad's chromatography media for ion exchange, affinity, size exclusion, and hydroxyapatite technology. They are designed for use with BioLogic systems, a peristaltic pump, or any other chromatography system. For simple step elution, the cartridges can be used with a syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

Micro Bio-Spin™ 6 Columns

732-6221EDU
Pkg of 25, prepacked spin columns with Bio-Gel® P-6 in Tris buffer, sample volume 10–75 μl, 6,000 MW limit, includes 50 collection tubes; education use only

List Price:   $116.00
EDU Price:   $92.80   What is this?
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Micro Bio-Spin 30 Columns

732-6223EDU
Pkg of 25, prepacked spin columns with Bio-Gel P-30 in Tris buffer, sample volume 10–75 μl, 40,000 MW limit, includes 50 collection tubes; education use only

List Price:   $116.00
EDU Price:   $92.80   What is this?
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Micro Bio-Spin 6, SSC

732-6200EDU
Pkg of 25, prepacked spin columns with Bio-Gel P-6 in SSC, sample volume 10–75 μl, MW exclusion limit 6,000, includes 50 collection tubes; education use only

List Price:   $116.00
EDU Price:   $92.80   What is this?
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Micro Bio-Spin 30,SSC

732-6202EDU
Pkg of 25, prepacked spin columns with Bio-Gel P-30 in SSC, sample volume 10–75 μl, 40,000 MW limit, includes 50 collection tubes; education use only

List Price:   $116.00
EDU Price:   $92.80   What is this?
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Bio-Spin 6 Columns

732-6227EDU
Pkg of 25, prepacked spin columns with Bio-Gel P-6 gel in Tris buffer, sample volume 50–100 μl, 6,000 MW limit, includes 50 collection tubes; education use only

List Price:   $144.00
EDU Price:   $115.20   What is this?
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Bio-Spin 30 Columns

732-6231EDU
Pkg of 25, prepacked spin columns with Bio-Gel P-30 in Tris buffer, sample volume 50–100 μl, MW exclusion limit 40,000, includes 50 collection tubes; education use only

List Price:   $144.00
EDU Price:   $115.20   What is this?
Quantity:   Add to cart
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Bio-Spin 6, SSC

732-6002EDU
Pkg of 25, prepacked spin columns with Bio-Gel P-6 in SSC, sample volume 50–100 μl, MW exclusion limit 6,000, includes 50 collection tubes; education use only

List Price:   $172.00
EDU Price:   $137.60   What is this?
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Bio-Spin 30, SSC

732-6006EDU
Pkg of 25, prepacked spin columns with Bio-Gel P-30 in SSC, sample volume 50–100 μl, MW exclusion limit 40,000, includes 50 collection tubes; education use only

List Price:   $172.00
EDU Price:   $137.60   What is this?
Quantity:   Add to cart
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Bio-Scale Mini UNOsphere Q Cartridge

732-4102EDU
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system; education use only

List Price:   $172.00
EDU Price:   $137.60   What is this?
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Bio-Scale Mini UNOsphere Q Cartridges

732-4100EDU
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system; education use only

List Price:   $243.00
EDU Price:   $194.40   What is this?
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Bio-Scale Mini UNOsphere Q Cartridges

732-4104EDU
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system; education use only

List Price:   $595.00
EDU Price:   $476.00   What is this?
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Bio-Scale Mini UNOsphere S Cartridge

732-4112EDU
Pkg of 1, 5 ml, prepacked cation exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems; education use only

List Price:   $172.00
EDU Price:   $137.60   What is this?
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Bio-Scale Mini UNOsphere S Cartridges

732-4110EDU
Pkg of 5, 1 ml, prepacked cation exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems; education use only

List Price:   $243.00
EDU Price:   $194.40   What is this?
Quantity:   Add to cart
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Bio-Scale Mini UNOsphere S Cartridges

732-4114EDU
Pkg of 5, 5 ml, prepacked cation exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems; education use only

List Price:   $595.00
EDU Price:   $476.00   What is this?
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Bio-Scale Mini Profinity IMAC Cartridge

732-4612EDU
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system; education use only

List Price:   $180.00
EDU Price:   $144.00   What is this?
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Bio-Scale™ Mini Profinity™ IMAC Cartridges

732-4610EDU
Pkg of 5, 1 ml, prepacked ion exchange column, 40 x 5.6 mm, max. pressure 45 psi, for use with BioLogic™ systems or any chromatography system; education use only

List Price:   $257.00
EDU Price:   $205.60   What is this?
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Bio-Scale Mini Profinity IMAC Cartridges

732-4614EDU
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system; education use only

List Price:   $634.00
EDU Price:   $507.20   What is this?
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Number Description Options
2303 Purification of Insulin on UNO S1 Column, Rev A Click to download
2284 Purification of Two Isoforms of Pyruvate Kinase from Nordic Krill Using an UNO Q1 Column, Rev A Click to download
2283 Purification of the Components of Human Macular Carotenoid-Binding Complex, Rev A Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5548 Profinia Protein Purification System Consumables and Accessories Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5514 Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A Click to download [ Add to Cart (Free) ]
5574 Bio-Scale Mini Profinity Cartridges Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5444 Bio-Scale Mini Profinity IMAC Cartridge Product Information Sheet, Rev A Click to download
4006051 Instruction Manual, Micro Bio-Spin Chromatography Columns, Rev B Click to download
4106140 Instruction Manual, Micro Bio-Spin Columns P-30 Tris, RNase Free, Rev C Click to download
LIT507 Instruction Manual, Bio-Spin 6 and 30 Columns, Rev G Click to download
4110104 Instruction Manual, Bio-Spin 30 Tris Columns, Rev C Click to download
2320 Bio-Spin and Micro Bio-Spin Column Product Information Sheet, Rev A Click to download