Bio-Scale™ Mini Affi-Prep® Protein A Cartridges

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Overview

Capacities of Protein A Supports
Immunoglobulin Affi-Gel Protein A
Capacity (mg/ml)
Affi-Prep Protein A
Capacity (mg/ml)
Mouse lgG1 6–10 7–9
Mouse lgG2a 6–10 7–9
Mouse lgG2b 6–10 7–9
Mouse lgG3 6–10 7–9
Mouse lgM* 6–10 7–9
Human lgG 15 10–12
Sheep lgG 8–10 7–9
Cow lgG 8–10 7–9
Horse lgG 8–10 7–9
Goat lgG 8–10 7–9
Rabbit lgG 8–10 7–9
Dog lgG 8–10 7–9
Pig lgG 8–10 7–9

* Approximately 50% of all mouse lgMs bind using the MAPS buffer system.

Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml–40 mm length x 5.6 mm inner diameter
5 ml–40 mm length x 12.6 mm inner diameter
10 ml–80 mm length x 12.6 mm inner diameter**
50 ml–80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732-4120 732-4122 732-4124
UNOsphere™ Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 732-4100 732-4102 732-4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732-4110 732-4112 732-4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732-4140 732-4142 732-4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732-4130 732-4132 732-4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732-4632 732-4634
Antibody Purification
Protein A Affi-Prep® Protein A Affinity Recombinant protein A 30 mg/ml human IgG
732-4200, 1 x 1 ml 732-4201, 5 x 1 ml 732-4202, 1 x 5 ml
Protein A Affi-Prep® Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600 732-4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 732-4322 732-4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 732-4332 732-4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
780-0811 780-0812
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732-4610 732-4612 732-4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732-4642 732-4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732-4502 732-4504 732-5304 732-5312 732-5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Protein Coupling Comparison

Protein coupling with Affi-Gel 10 and Affi-Gel 15 gels. Coupling conditions: Each protein solution (3 ml of 0.1 M MOPS, pH 7.5, containing 40 mg protein) was combined with 2 ml of Affi-Gel gel.

Bio-Scale Mini Affi-Prep protein A cartridges are 1 ml and 5 ml cartridges filled with Affi-Prep protein A support. Affi-Prep protein A support can be used to yield highly purified immunoglobulins (IgGs), selectively remove IgG prior to analysis of other immunoglobulin classes, or adsorb immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species. The cartridges can be used for method scouting or simple purifications.

Advantages include:

  • Binds a wide range of monoclonal and polyclonal antibodies, including mouse IgG1
  • Macroporous polymeric support is not compressible under normal operating conditions
  • Compatible with most commonly used chromatographic buffers
  • Stable pH range from 2–10
  • Multiple chromatographic cycles without loss of capacity
  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer
  • Easy to use

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, disposable, and ready to use, these cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems.

For more information, request bulletins 5326 and 5584.

* U.S. Patent 7,208,087.

Bio-Scale Mini Affi-Prep Protein A Cartridges

732-4600
Pkg of 5, 1 ml, prepacked affinity column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $769.00
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Bio-Scale Mini Affi-Prep Protein A Cartridge

732-4602
Pkg of 1, 5 ml, prepacked affinity column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

List Price:   $647.00
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Number Description Options
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download
5701 Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B Click to download [ Add to Cart (Free) ]
5726 Profinia Protein A and G methods Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5712 The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A Click to download [ Add to Cart (Free) ]
LIT230 Instruction Manual, Affi-Prep Protein A Matrix Click to download
LIT79 Instruction Manual, Affi-Prep Protein A MAPS II Kit Click to download
10006546 Bio-Scale Mini Affi-Prep® Protein A Cartridges, 1 and 5 ml, Instruction Manual, Rev C Click to download