PDS-1000 | He™ System

PDS-1000 | He™ System

Overview

The PDS-1000/He system uses a burst of high-pressure helium gas to accelerate nucleic acid–coated gold or tungsten microparticles to velocities necessary to transfect cells, tissues, or organelles.

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Description

Description

PDS-1000/He™ System

The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.

The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells

Key Features

  • A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
  • Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
  • Maximum control
  • High reproducibility

 

PDS-1000 / He™ and Hepta™ Training Video. This video provides an overview of the PDS-1000 / He™ and Hepta™ Biolistic Particle Transfection Systems, including instrument setup, consumable preparation, instrument operation and shut down.

Specifications

Specifications

Dimensions (W x D x H)
29 x 25.5 x 47.5 cm
Construction
Aluminum, ABS plastic, and acrylic chassis
Weight
15 kg
Electrical input voltage
100–120 VAC, 50–60 Hz
Maximum current
<5 A
Mechanical
 
Fuse
6.3 A, 250 V, 5 x 20 mm
Vacuum
<0.4 inches mercury/min leakage
Overpressure
0.5 psi relief valve, self-resetting
Environmental
 
Operating
Temperature: 0–35°C (32–95°F)
Humidity: 0–95% noncondensing
Storage
Temperature: 0–70°C (32–158°F)
Humidity: 0–95% noncondensing

Use

For Research Use Only. Not for use in diagnostic procedures.

Ordering

Ordering

items

Use the filters below to refine results!

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165-2257_tn.jpg
(Discontinued) PDS-1000/He System
1652257
100/120 V, biolistic transformation system, includes helium pressure regulator, solenoid, spacer rods, microcarrier launch assembly, target shelf, 5 macrocarrier holders, tubing

Accessories

Accessories

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(Discontinued) 500 Optimization Kit
1652278

Pkg of 1, optimization kit for the PDS-1000/He system, includes 0.25 g each of 0.6 μm, 1.0 μm, and 1.6 μm gold microcarriers, 100 each of 7 rupture disks, for 500 bombardments

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165-2329_tn.jpg
1652329
Pkg of 100, bursts at 1,100 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2330_tn.jpg
1652330
Pkg of 100, bursts at 1,350 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
Image
165-2331_tn.jpg
1652331
Pkg of 100, bursts at 1,550 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
Image
165-2332_tn.jpg
1652332
Pkg of 100, bursts at 1,800 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Documents

Documents

Number Description Options
5443
Biolistic Particle Delivery Systems Brochure, Rev A
5447
Biolistic PDS-1000/He System Flier, Rev A
10000070900
Instruction Manual, PDS-1000/He Biolistic Particle Delivery System
4100150
Instruction Manual, Safety Addendum for the Biolistic PDS-1000/He Particle Delivery System, Rev A
4106154
Instruction Manual, Hepta Adaptor, PDS-1000/He Biolistic Particle Delivery System, Rev A
2015
Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cell Types
2007
Bombardment-Mediated Transformation Methods for Barley
2051
Transformation of Filamentous Fungi by Microprojectile Bombardment
2658
Single-Cell Complementation of Barley mlo Mutants Using a PDS-1000/He Hepta System
2087
Biolistic Transfection of Organotypic Brain Slices and Dissociated Cells
2453
Optimization of Gene Delivery Into Arabidopsis, Tobacco, and Birch Using the Helios Gene Gun System
1688
Optimization of Biolistic® Transformation Using the Helium-Driven PDS-1000/He System
1689
Comparison of Performance Characteristics of Different Biolistic® Devices
2768
Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A