Nuvia™ S Media

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Overview

Media Comparison: Dynamic Binding Capacity

 

 

Binding of polyclonal human IgG by Nuvia S media. Comparison of Nuvia S and other commercially available CEX media. Column size, 0.7 x 5.5 cm. The sample was loaded onto the column in 40 mM sodium acetate, pH 5.0 and 30 mM sodium chloride, washed, and eluted with 40 mM sodium acetate, pH 5.0 and 1 M sodium chloride.

DBC, Dynamic Binding Capacity. BT, Breakthrough

Ultra-high binding capacities provide users with the maximum value through reduced media and buffer consumption, when used in both capture and polish mode.

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Nuvia™ Q and S Ultra-High Capacity Ion Exchange Media

Bio-Rad now offers two ultra-high capacity ion exchangers: Nuvia S, which can be used for capture and/or intermediate polishing, and Nuvia Q, which can be used for intermediate and/or final polishing.

Best-in-Class Dynamic Binding Capacity

 


 

Binding of a monoclonal antibody (mAb) by Nuvia S media. Column size, 1.1 cm x 10 cm; sample, 4.5–5.0 mg/ml mAb, was loaded onto the column in 20 mM sodium acetate, pH 5.0 + sodium chloride (adjusted for conductivity) until 5% and 10% breakthrough capacity were observed.

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Nuvia S Superior Flow Properties

 

Nuvia

 

Dynamic binding capacity (DBC) vs. flow velocity. A 1.1 cm diameter column was packed to a 20 cm bed height with Nuvia S media. 4.5 mg/ml human polyclonal IgG in 40 mM sodium acetate and 30 mM sodium chloride, pH 5.0 was loaded until 10% breakthrough was observed.

Nuvia S Media Chemical Stability

 


 

Stability of Nuvia S media. Results from an accelerated storage study (in 1.0 M NaOH) show no loss in dynamic binding capacity and recovery.

Base-Stability, Consistent Performance
The chemical stability of Nuvia S media allows the media to withstand repeated exposure to commonly used sanitization and clean-in-place protocols. Nuvia S media exposed to 840 hr of 1.0 M NaOH with typically used cleaning cycles showed no decreases of dynamic binding capacity or recovery. Nuvia S media delivers value and process stability by maintaining peak performance over an extended life.

Nuvia S is an ultra-high capacity, next-generation cation exchange media built on the industry proven UNOsphere™ base matrix technology. Nuvia S media provides very high capture and exceptional flow properties designed to meet current and future process needs. Nuvia S media is a flexible alternative that may be used both as a capture or polishing solution. The media delivers superior binding capacity over a wide operational window, making it the tool of choice for a wide range of downstream processes.

Nuvia S media delivers value and flexibility by allowing users to:

  • Use less media to purify a given amount of product
  • Reduce cycle time and increase productivity by operating at higher flow rates
  • Reduce costs and space requirements by decreasing buffer consumption
  • Reduce capital and operating expenses through the use of smaller columns
  • Chemical stability to withstand repetitive clean-in-place cycles
  • Flexibility in use for capture or polish steps
  • Fully supported for regulatory submission
Type of ion exchanger Strong cation
Functional group –SO3
Total ionic capacity 90–150 µeq/ml
Dynamic binding capacity*: 300 cm/hr ≥110 mg/ml
Shipping counterion Na+
Median particle size 85 ± 15 µm
Operational flow rate** 50–600 cm/hr
Chemical stability: 1.0 N NaOH (20ºC) Up to 1 week
Chemical stability: 0.1 N NaOH (20ºC)*** Up to 5 years
Gel bed compression ratio 1.15–1.18 (settled bed volume/packed bed volume)
pH stability short-term*** 2–14
pH stability long-term*** 4–13
Shipping solution 20% ethanol + 0.1 M NaCl
Regeneration 1–2 M NaCl
Sanitation 0.5–1.0 N NaOH
Storage Conditions 20% ethanol or 0.1 N NaOH
   
* 10% breakthrough capacity determined with 4.5 mg/ml human
IgG in 40 mM NaOAc + 30 mM NaCl, pH 5.0.
 
** Recommended elution flow rates are ≤ 150 cm/hr to minimize viscosity-induced backpressure.  
*** Data derived under accelerated conditions at 60°C.  
Nuvia S Media, 25 ml #156-0311
Nuvia™ S Media

156-0311
25 ml, strong cation exchange chromatography media, 85 μm particle size

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Nuvia S Media, 100 ml #156-0313
Nuvia™ S Media

156-0313
100 ml, strong cation exchange chromatography media, 85 μm particle size

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Nuvia S Media, 500 ml #156-0315
Nuvia™ S Media

156-0315
500 ml, strong cation exchange chromatography media, 85 μm particle size

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Foresight™ Nuvia™ S Plates

732-4701
2 x 96-well plates, 20 μl, filter plate prepacked with Nuvia S strong cation exchange chromatography media

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Foresight™ Nuvia™ S RoboColumn Unit

732-4801
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with Nuvia S strong cation exchange chromatography media

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Foresight™ Nuvia™ S RoboColumn Unit

732-4802
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with Nuvia S strong cation exchange chromatography media

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Foresight™ Nuvia™ S Column

732-4720
Pkg of 1, 1 ml, column prepacked with Nuvia S strong cation exchange chromatography media

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Foresight™ Nuvia™ S Column

732-4740
Pkg of 1, 5 ml, column prepacked with Nuvia S strong cation exchange chromatography media

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Number Description Options
RP0049 The Effect of Conductivity on Dynamic Binding Capacity on CEX Resins Tech Poster, Rev A. Click to download [ Add to Cart (Free) ]
RP0048 How to Choose an Industrial Cation Exchanger for IgG Purification Click to download
5984 Nuvia S Media: A High-Capacity Cation Exchanger for Process Purification of Monoclonal Antibodies, Rev A Click to download
5987 Nuvia S Cation Exchange Media Product Information Sheet, Rev B Click to download
6129 Nuvia Q Anion Exchange Media Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
10018215 Instruction Manual, Nuvia S and Q High-Capacity Ion Exchange Media, Rev C Click to download [ Add to Cart (Free) ]
10018528 Instruction Manual, Bio-Scale Nuvia S Cartridges 1 and 5 ml, Rev A Click to download
6242 Nuvia cPrime Hydrophobic Cation Exchange Media Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]