Nitrocellulose Membrane, 0.45 µm

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Overview

Bio-Rad Protein Transfer Membrane Offerings
Protein Membranes

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Immun-Blot® PVDF Membrane
  • Ideal for chemiluminescent and colorimetric western blots
  • Retains target protein very strongly during protein transfer but also reduces nonspecific protein binding
Immun-Blot® Low Fluorescence PVDF Membrane
  • Same benefits as standard PVDF membranes plus minimal autofluorescence and high sensitivity
  • Best choice for multiplex fluorescent immunoblotting
Sequi-Blot™ PVDF Membrane
  • Ideal for protein sequencing: retains target protein very strongly during protein transfer
  • Provides the binding capacity to sequence even low-abundance samples
Nitrocellulose Membrane, 0.45 µm
  • Proven medium for western, northern, and Southern blotting
  • 45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers
  • 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins
Nitrocellulose Membrane, 0.2 µm
Supported Nitrocellulose Membrane, 0.45 µm
  • Dense 100% nitrocellulose cast on both sides of an inert polyester support
  • Easy to handle, wets readily, and does not have the brittleness often associated with unsupported nitrocellulose
Supported Nitrocellulose Membrane, 0.2 µm
Nitrocellulose Membranes

Ideal for:

  • Protein blotting
  • DNA/RNA blotting
  • Dot/slot blot screening
  • Colony/plaque screening

 

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.



Bio-Rad's 0.45 µm pore–size nitrocellulose membrane is dense 100% nitrocellulose. Pure nitrocellulose is the most frequently used membrane for the transfer of both proteins and nucleic acids. It is a proven medium for western, northern, and Southern blotting that produces excellent signal-to-noise results. Bio-Rad nitrocellulose is easy to handle, wets readily with water-based solutions, and resists the brittleness usually associated with nitrocellulose. The 0.45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers.

Features and Benefits

  • Compatible with all standard immunoblotting and nucleic acid detection methods
  • Provides superior binding capacity without background interference
  • Pore size produces excellent signal-to-noise blotting results
  • Available in three formats:
    • Convenient precut 0.45 µm membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transfer of protein from SDS-PAGE gels to membrane in preparation for western blotting
  • Transfer of a wide range of protein molecular weights and nucleic acids >500 bp
  • Transfer of nucleic acids in preparation for northern or Southern blotting
  • Immunodetection of target proteins and probe hybridization of nucleic acids

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

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Waste Electrical and Electronic Equipment ("WEEE Directive")

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Nitrocellulose Membrane #162-0115
Nitrocellulose Membrane, Roll, 0.45 µm, 30 cm x 3.5 m

1620115
Pkg of 1 roll, 0.45 μm, 30 cm x 3.5 m, bulk blotting membrane for proteins and nucleic acids; can be used for custom sizing

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$321.00
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Nitrocellulose Membranes #162-0113
Nitrocellulose Membrane, Precut, 0.45 µm, 20 x 20 cm

1620113
Pkg of 5, 0.45 µm, 20 x 20 cm, precut blotting membrane for proteins and nucleic acids

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$152.00
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Nitrocellulose Membranes #162-0116
Nitrocellulose Membrane, Precut, 0.45 µm, 15 x 15 cm

1620116
Pkg of 10, 0.45 µm, 15 x 15 cm, precut blotting membrane for proteins and nucleic acids

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$169.00
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Nitrocellulose Membranes #162-0114
Nitrocellulose Membrane, Precut, 0.45 µm, 15 x 9.2 cm

1620114
Pkg of 10, 0.45 µm, 15 x 9.2 cm, precut blotting membrane for proteins and nucleic acids

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$120.00
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Nitrocellulose Membranes #162-0148
Nitrocellulose Membrane, Precut, 0.45 µm, 11.5 x 16 cm

1620148
Pkg of 10, 0.45 µm, 11.5 x 16 cm, precut blotting membrane for proteins and nucleic acids

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$158.00
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Nitrocellulose Membranes #162-0117
Nitrocellulose Membrane, Precut, 0.45 µm, 9 x 12 cm

1620117
Pkg of 10, 0.45 µm, 9 x 12 cm, precut blotting membrane for proteins and nucleic acids

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$118.00
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Nitrocellulose Membranes #162-0145
Nitrocellulose Membrane, Precut, 0.45 µm, 7 x 8.5 cm

1620145
Pkg of 10, 0.45 µm, 7 x 8.5 cm, precut blotting membrane for proteins and nucleic acids

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$77.00
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Nitrocellulose Membranes #162-0167
Nitrocellulose Membrane, Precut, 0.45 µm, 8.5 x 13.5 cm

1620167
Pkg of 10, 0.45 µm, 8.5 x 13.5 cm, precut blotting membrane for proteins and nucleic acids

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$118.00
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Nitrocellulose Membranes #162-0251
Nitrocellulose Membrane, Precut, 0.45 µm, 26.5 x 28 cm

1620251
Pkg of 10, 0.45 µm, 26.5 x 28 cm, precut blotting membrane for proteins and nucleic acids

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$218.00
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Nitrocellulose/Filter Paper Sandwiches #162-0234
Nitrocellulose/Filter Paper Sandwiches

1620234
Pkg of 20, 0.45 μm, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Criterion and Criterion XT gels

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$275.00
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Nitrocellulose/Filter Paper Sandwiches #162-0235
Nitrocellulose/Filter Paper Sandwiches

1620235
Pkg of 50, 0.45 μm, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Criterion and Criterion XT gels

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$599.00
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Nitrocellulose/Filter Paper Sandwiches #162-0214
Nitrocellulose/Filter Paper Sandwiches

1620214
Pkg of 20, 0.45 μm, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Mini-PROTEAN® and Ready Gel® precast gels

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$177.00
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Nitrocellulose/Filter Paper Sandwiches #162-0215
Nitrocellulose/Filter Paper Sandwiches

1620215
Pkg of 50, 0.45 μm, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Mini-PROTEAN® and Ready Gel® precast gels

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$381.00
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Number Description Options
2895 Protein Blotting Guide, Ver C Click to download
1939 Blotting Membrane Brochure, Rev G Click to download