Nitrocellulose Membrane, 0.45 µm

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Overview

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Trans-Blot® Turbo™ Transfer System

The Trans-Blot Turbo system is a fast, efficient, and reproducible transfer system for transferring proteins from gels to membranes in as little as 3 minutes.

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Available Formats

Blotting membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Nitrocellulose Membranes

Ideal for:

  • Protein blotting
  • DNA/RNA blotting
  • Dot/slot blot screening
  • Colony/plaque screening

 

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

Bio-Rad's 0.45 µm pore–size nitrocellulose membrane is dense 100% nitrocellulose. Pure nitrocellulose is the most frequently used membrane for the transfer of both proteins and nucleic acids. It is a proven medium for western, northern, and Southern blotting that produces excellent signal-to-noise results. Bio-Rad nitrocellulose is easy to handle, wets readily with water-based solutions, and resists the brittleness usually associated with nitrocellulose. The 0.45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers.

Features and Benefits

  • Compatible with all standard immunoblotting and nucleic acid detection methods
  • Provides superior binding capacity without background interference
  • Pore size produces excellent signal-to-noise blotting results
  • Available in three formats:
    • Convenient precut 0.45 µm membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transfer of protein from SDS-PAGE gels to membrane in preparation for western blotting
  • Transfer of a wide range of protein molecular weights and nucleic acids >500 bp
  • Transfer of nucleic acids in preparation for northern or Southern blotting
  • Immunodetection of target proteins and probe hybridization of nucleic acids

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

Nitrocellulose Membrane #162-0115
Nitrocellulose Membrane

162-0115
Pkg of 1 roll, 0.45 μm, 30 cm x 3.5 m, bulk blotting membrane for proteins and nucleic acids; can be used for custom sizing

List Price:   $299.00
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Nitrocellulose Membranes #162-0113
Nitrocellulose Membranes

162-0113
Pkg of 5, 0.45 μm, 20 x 20 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $136.00
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Nitrocellulose Membranes #162-0116
Nitrocellulose Membranes

162-0116
Pkg of 10, 0.45 μm, 15 x 15 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $149.00
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Nitrocellulose Membranes #162-0114
Nitrocellulose Membranes

162-0114
Pkg of 10, 0.45 μm, 15 x 9.2 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $114.00
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Nitrocellulose Membranes #162-0148
Nitrocellulose Membranes

162-0148
Pkg of 10, 0.45 μm, 11.5 x 16 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $142.00
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Nitrocellulose Membranes #162-0117
Nitrocellulose Membranes

162-0117
Pkg of 10, 0.45 μm, 9 x 12 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $112.00
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Nitrocellulose Membranes #162-0145
Nitrocellulose Membranes

162-0145
Pkg of 10, 0.45 μm, 7 x 8.5 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $74.00
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Nitrocellulose Membranes #162-0167
Nitrocellulose Membranes

162-0167
Pkg of 10, 0.45 μm, 8.5 x 13.5 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $112.00
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Nitrocellulose Membranes #162-0251
Nitrocellulose Membranes

162-0251
Pkg of 10, 0.45 μm, 26.5 x 28 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $204.00
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Nitrocellulose/Filter Paper Sandwiches #162-0234
Nitrocellulose/Filter Paper Sandwiches

162-0234
Pkg of 20, 0.45 μm, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Criterion and Criterion XT gels

List Price:   $244.00
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Nitrocellulose/Filter Paper Sandwiches #162-0235
Nitrocellulose/Filter Paper Sandwiches

162-0235
Pkg of 50, 0.45 μm, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Criterion and Criterion XT gels

List Price:   $546.00
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Nitrocellulose/Filter Paper Sandwiches #162-0214
Nitrocellulose/Filter Paper Sandwiches

162-0214
Pkg of 20, 0.45 μm, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:   $156.00
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Nitrocellulose/Filter Paper Sandwiches #162-0215
Nitrocellulose/Filter Paper Sandwiches

162-0215
Pkg of 50, 0.45 μm, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:   $347.00
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Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]