Nitrocellulose Membrane, 0.2 µm

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Overview

Available Formats

Blotting membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Nitrocellulose Membranes

Ideal for:

  • Protein blotting
  • DNA/RNA blotting
  • Dot/slot blot screening
  • Colony/plaque screening

 

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

Bio-Rad's 0.2 µm pore–size nitrocellulose membrane is dense 100% nitrocellulose. Pure nitrocellulose is the most frequently used membrane for the transfer of both proteins and nucleic acids. It is a proven medium for western, northern, and Southern blotting that produces excellent signal-to-noise results. Bio-Rad nitrocellulose is easy to handle, wets readily with water-based solutions, and resists the brittleness usually associated with nitrocellulose. The 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins: the smaller pore size minimizes protein sample loss due to transfer through the membrane.

Features and Benefits

  • Compatible with all standard immunoblotting and nucleic acid detection methods
  • Provides superior binding capacity without background interference
  • Smaller pore size minimizes low molecular–weight protein sample loss
  • Available in three formats:
    • Convenient precut 0.2 µm membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transfer of low molecular–weight proteins and nucleic acids <500 bp
  • Protein transfer from SDS-PAGE gels to membrane in preparation for western blotting
  • Transfer of nucleic acids in preparation for northern or Southern blotting
  • Immunodetection of target protein and probe hybridization of nucleic acids

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different membrane types and size selections by gel type.

Nitrocellulose Membrane

162-0112
Pkg of 1 roll, 0.2 μm, 30 cm x 3.5 m, bulk blotting membrane for proteins and nucleic acids

List Price:   $378.00
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Nitrocellulose Membranes #162-0150
Nitrocellulose Membranes

162-0150
Pkg of 5, 0.2 μm, 20 x 20 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $192.00
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Nitrocellulose Membranes #162-0147
Nitrocellulose Membranes

162-0147
Pkg of 10, 0.2 μm, 13.5 x 16.5 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $230.00
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Nitrocellulose Membranes #162-0146
Nitrocellulose Membranes

162-0146
Pkg of 10, 0.2 μm, 7 x 8.4 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $80.00
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Nitrocellulose Membranes #162-0168
Nitrocellulose Membranes

162-0168
Pkg of 10, 0.2 μm, 8.5 x 13.5 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $115.00
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Nitrocellulose Membranes #162-0252
Nitrocellulose Membranes

162-0252
Pkg of 10, 0.2 μm, 26.5 x 28 cm, precut blotting membrane for proteins and nucleic acids

List Price:   $261.00
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Nitrocellulose/Filter Paper Sandwiches #162-0232
Nitrocellulose/Filter Paper Sandwiches

162-0232
Pkg of 20, 0.2 μm, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Criterion and Criterion XT gels

List Price:   $244.00
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Nitrocellulose/Filter Paper Sandwiches #162-0233
Nitrocellulose/Filter Paper Sandwiches

162-0233
Pkg of 50, 0.2 μm, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Criterion and Criterion XT gels

List Price:   $560.00
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Nitrocellulose Membrane #162-0112
Nitrocellulose/Filter Paper Sandwiches

162-0212
Pkg of 20, 0.2 μm, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:   $172.00
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Nitrocellulose/Filter Paper Sandwiches #162-0213
Nitrocellulose/Filter Paper Sandwiches

162-0213
Pkg of 50, 0.2 μm, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:   $370.00
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Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]