MPC™ Ceramic Hydroxyfluoroapatite Media

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MPC™ Ceramic Hydroxyfluoroapatite Media

Overview

Comparative Elution Profile of a Protein Mixture with MPC and CHT Media
Elution Profile
 

Separation of a protein mixture of ovalbumin, myoglobulin, alpha-chymotrypsinogen A, and cytochrome C with MPC () and CHT Type I, 40 µm () media yielded similar results.

To learn more about MPC media, download our product information sheet.

Monoclonal Antibody Purification and Quantification Using MPC and CHT Media

MAb-S Purification Profile

MAb-S Purification Profile

Fig. 1A. The chromatographic performance for purifying the MAb-S sample with MPC ( ) and CHT Type I, 40 µm () shows separation of the monomer from higher molecular weight impurities.


SEC Profile of Pooled Monomer Fractions

SEC Profile of Pooled Monomer Fractions

 

Fig. 1B. The elution pools collected on the MAb-S antibody sample purified on MPC and CHT Type I, 40 µm (in Figure 1A above) were analyzed for purity by size exclusion chromatography. The overlaid chromatograms of the elution pools show only one major peak and no evidence of dimers or aggregates in the MPC ( ) or CHT () separations.


Data Quantification

  IgG concentration, mg/ml Protein A, ppm HCP, ppm DNA, ppm Dimer/aggregate, %
MAb-S          
Protein A eluate  2.90 33 24 21 21.62
CHT pool 0.40 <0.6 <2 2.62 <0.03
MPC pool 0.46 <0.5 <2 2.83 <0.03

Table 1. All pooled fractions and starting material from MAb-S purified on MPC and CHT Type I, 40 µm (Figure 1B above) were analyzed for host cell protein using a Cygnus Technologies HCP ELISA kit, for dsDNA using a PicoGreen dsDNA quantitation kit from Molecular Probes, Inc., for Protein A leachables using a Cygnus Technologies Protein A ELISA kit, and for aggregate clearance by size exclusion chromatography on a Bio-Sil® SEC 250 column.

To learn more about MPC media, download our product information sheet.

MPC ceramic hydroxyfluoroapatite, a composite of hydroxyapatite and fluoroapatite (Ca10(PO4)6(OH)1.5(F)0.5), is a complementary addition to our line of ceramic apatite mixed-mode chromatography media. This second-generation CHT Type I, 40 µm media confers greater pH stability, providing better process economics to the biopharmaceutical scientist. MPC has the unique separation properties and unmatched selectivity and resolution of CHT.

MPC delivers value by providing:

  • High physical and chemical stability
  • Exceptional separation of contaminants and aggregates in a single step
  • Simple and rapid column packing

The addition of MPC displays our commitment to our ceramic apatite business and further builds upon our expertise in mixed-mode chromatography. Request a free sample of MPC.

For more information, consult the CHT application guide and product information sheet.
Bio-Rad Laboratories is an ISO 9001 registered corporation.

Functional groups Ca2+, PO4, OH, F
Observed dynamic binding capacity lysozyme (Lys) ≥25 mg Lys/g MPC
Nominal pore diameter 600–800 Å
Maximum backpressure 100 bar (1,500 psi)
Nominal mean particle size 40 ± 4 µm
Bulk density 0.72 g/ml
Foresight™ MPC™ Type I Plates, 40 µm, 20 µl
Foresight™ MPC™ Type I Plates, 40 µm, 20 µl

732-4785
2 x 96-well plates, 20 µl, filter plate prepacked with MPC Type I mixed-mode chromatography resin, 40 µm particle size

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Foresight™ MPC™ Type I Column, 40 µm, 1 ml
Foresight™ MPC™ Type I Column, 40 µm, 1 ml

732-4737
Pkg of 1, 1 ml, column prepacked with MPC Type I mixed-mode chromatography resin, 40 µm particle size

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Foresight™ MPC™ Type I Column, 40 µm, 5 ml
Foresight™ MPC™ Type I Column, 40 µm, 5 ml

732-4757
Pkg of 1, 5 ml, column prepacked with MPC Type I mixed-mode chromatography resin, 40 µm particle size

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Foresight™ MPC™ Type I RoboColumn Unit, 40 µm, 200 µl
Foresight™ MPC™ Type I RoboColumn Unit, 40 µm, 200 µl

732-4828
1 row of 8 columns, 200 µl, RoboColumn unit prepacked with MPC Type I mixed-mode chromatography resin, 40 µm particle size

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Foresight™ MPC™ Type I RoboColumn Unit, 40 µm, 600 µl
Foresight™ MPC™ Type I RoboColumn Unit, 40 µm, 600 µl

732-4829
1 row of 8 columns, 600 µl, RoboColumn unit prepacked with MPC Type I mixed-mode chromatography resin, 40 µm particle size

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MPC™ Ceramic Hydroxyfluoroapatite

158-0200
10 g, MPC ceramic hydroxyapatite mixed-mode chromatography media, Type I, 40 µm particle size

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MPC™ Ceramic Hydroxyfluoroapatite

157-0200
100 g, MPC ceramic hydroxyapatite mixed-mode chromatography media, Type I, 40 µm particle size

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MPC™ Ceramic Hydroxyfluoroapatite

157-0201
1 kg, MPC ceramic hydroxyapatite mixed-mode chromatography media, Type I, 40 µm particle size

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MPC™ Ceramic Hydroxyfluoroapatite

157-0205
5 kg, MPC ceramic hydroxyapatite mixed-mode chromatography media, Type I, 40 µm particle size

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Number Description Options
6432 MPC Ceramic Hydroxyfluoroapatite, Rev B Click to download
6086 Ceramic Hydroxyapatite Application Guide for Process Development and Scale-Up, Rev C Click to download [ Add to Cart (Free) ]
5667 CHT Ceramic Hydroxyapatite Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]