Molecular Rulers

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Bio-Rad molecular rulers are DNA standards with precisely defined size intervals between bands (DNA ladders) for simplified estimation of the length of nucleic acids separated on agarose gels. Bio-Rad provides three types of molecular rulers: standard, EZ Load, and AmpliSize rulers. To ensure a precise measurement of the size of your samples, most of these DNA ladders include a visually distinct reference band.

Standard Molecular Rulers

Standard molecular rulers consist of precisely sized DNA and are provided ready for dilution. Each vial contains DNA for 100 applications. The advantages of standard molecular rulers include:

  • Visually distinct reference bands
  • Five size ranges to select from
  • No contaminating plasmid DNA

EZ Load Molecular Rulers

Ready-to-load EZ Load molecular rulers consist of precisely sized DNA blended with loading buffer for added convenience (prediluted to a concentration appropriate for most electrophoresis runs). Each vial contains DNA for 100 applications and includes an additional vial of 5x sample buffer for preparing your own samples. The advantages of EZ Load molecular rulers include:

  • No preparation — ready for direct loading on agarose or polyacrylamide gels
  • Visually distinct reference bands
  • Five size ranges to select from
  • No contaminating plasmid DNA

AmpliSize Molecular Ruler

The AmpliSize molecular ruler contains blunt-ended DNA of known sequence at 50% GC content. It has ten bands (at 50, 100, 200, 300, 400, 500, 700, 1,000, 1,500, and 2,000 bp). Each vial contains DNA for 50 applications.

20 bp Molecular Ruler  
Contents 1 vial 20 bp molecular ruler, 250 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)
Number of applications 100 when used at 2.5 µl per load
Concentration 0.2 µg/µl
Amount of DNA 50 µg DNA
Number of bands 50 in 20 bp increments
Reference bands 200 bp contains 3x the concentration of material found in the other bands
EZ Load™ 20 bp Molecular Ruler  
Contents  1 vial EZ Load 20 bp molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF
Number of applications  100 when used at 5 µl per load
Concentration  0.1 µg/µl
Amount of DNA  50 µg DNA  
Number of bands  50 in 20 bp increments  
Reference bands  200 bp contains 3x the concentration of material found in the other bands 
100 bp Molecular Ruler  
Contents   1 vial 100 bp molecular ruler, 250 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)
Number of applications   100 when used at 2.5 µl per load  
Concentration   0.1 µg/µl  
Amount of DNA   25 µg DNA  
Number of bands   10 in 100 bp increments  
Reference bands   None
EZ Load™ 100 bp Molecular Ruler  
Contents    1 vial EZ Load 100 bp molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF
Number of applications 100 when used at 5 µl per load   
Concentration 0.05 µg/µl   
Amount of DNA    25 µg DNA    
Number of bands    10 in 100 bp increments    
Reference bands    None   
100 bp PCR Molecular Ruler                         
Contents             1 vial PCR molecular ruler, 200 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)            
Number of applications             100 when used at 2 µl per load            
Concentration             0.2 µg/µl            
Amount of DNA             40 µg DNA            
Number of bands             30 in 100 bp increments            
Reference bands             1,000 and 3,000 bp            
EZ Load 100 bp PCR Molecular Ruler  
Contents     1 vial EZ Load 100 bp PCR molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF    
Number of applications     100 when used at 5 µl per load    
Concentration     0.08 µg/µl    
Amount of DNA     40 µg DNA    
Number of bands     30 in 100 bp increments     
Reference bands     1,000 bp and 3,000 bp    
500 bp Molecular Ruler      
Contents      1 vial 500 bp molecular ruler, 200 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)     
Number of applications      100 when used at 2 µl per load     
Concentration      0.2 µg/µl     
Amount of DNA      40 µg DNA      
Number of bands      16 in 500 bp increments      
Reference band      5,000 bp     
EZ Load 500 bp Molecular Ruler             
Contents       1 vial EZ Load 500 bp molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF      
Number of applications       100 when used at 5 µl per load      
Concentration       0.08 µg/µl      
Amount of DNA       40 µg DNA       
Number of bands       16 in 500 bp increments       
Reference bands       5,000 bp      
1 kb Molecular Ruler               
Contents        1 vial of 1 kb molecular ruler, 200 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)       
Number of applications        100 when used at 2 µl per load       
Concentration        0.2 µg/µl       
Amount of DNA        40 µg DNA        
Number of bands        15 in 1 kb increments        
Reference bands        5 kb       
EZ Load 1 kb Molecular Ruler                 
Contents           1 vial EZ Load 1 kb molecular ruler, 500 µl supplied in 5% glycerol, 15 mM Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol blue, 0.04% xylene cyanole FF          
Number of applications           100 when used at 5 µl per load          
Concentration           0.08 µg/µl          
Amount of DNA           40 µg DNA           
Number of bands           15 in 1 kb increments           
Reference bank           5 kb          
AmpliSize® Molecular Ruler                       
Contents            10 fragments of proprietary blunt-ended DNA of precise length and known sequence           
Number of applications            50 when used at 5 μl per load           
Concentration            0.1 µg/µl (10 ng/band/µl)           
Amount of DNA            25 µg DNA            
Reference bands            None           
2.5 kb Molecular Ruler                         
Contents             1 vial 2.5 kb molecular ruler, 400 µl supplied in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8)            
Number of applications             100 when used at 4 µl per load            
Concentration             0.1 µg/µl            
Amount of DNA             40 µg DNA             
Number of bands             14 in 2.5 kb increments            
Reference band             10 kb            
20 bp Molecular Ruler

170-8201
250 μl, 0.2 μg/μl, DNA standard, 20–1,000 bp, 50 bands in 20 bp increments, 100 applications

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EZ Load™ 20 bp Molecular Ruler

170-8351
500 μl, 0.1 μg/μl, ready-to-load DNA standard, 20–1,000 bp, 50 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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100 bp Molecular Ruler

170-8202
250 μl, 0.1 μg/μl, DNA standard, 100–1,000 bp, 10 bands in 100 bp increments, 100 applications

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EZ Load™ 100 bp Molecular Ruler

170-8352
500 μl, 0.05 μg/μl, ready-to-load DNA standard, 100–1,000 bp, 10 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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100 bp PCR Molecular Ruler

170-8206
200 μl, 0.2 μg/μl, DNA standard, 100–3,000 bp, 30 bands in 100 bp increments, 100 applications

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EZ Load™ 100 bp PCR Molecular Ruler

170-8353
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 100–3,000 bp, 30 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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500 bp Molecular Ruler

170-8203
200 μl, 0.2 μg/μl, DNA standard, 500–8,000 bp, 16 bands in 500 bp increments, 100 applications

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EZ Load™ 500 bp Molecular Ruler

170-8354
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 500–8,000 bp, 16 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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1 kb Molecular Ruler

170-8204
200 μl, 0.2 μg/μl, DNA standard, 1–15 kb, 15 bands in 1 kb increments, 100 applications

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EZ Load™ 1 kb Molecular Ruler

170-8355
500 μl, 0.08 μg/μl, ready-to-load DNA standard, 1–15 kb, 15 bands, includes 1 ml 5x nucleic acid sample buffer, 100 applications

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2.5 kb Molecular Ruler

170-8205
400 μl, 0.1 μg/μl, DNA standard, 2.5–35 kb, 14 bands in 2.5 kb increments, 100 applications

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AmpliSize® Molecular Ruler

170-8200
250 μl, 0.1 μg/μl, DNA standard at 50% GC content, 50–2,000 bp, 10 bands, 50 applications

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Number Description Options
4100090 Instruction Manual, 2.5 kb Molecular Ruler Click to download
4100089 Instruction Manual, 1 kb Molecular Ruler Click to download
4100088 Instruction Manual, 500 bp Molecular Ruler Click to download
4100087 Instruction Manual, 100 bp PCR Molecular Ruler Click to download
4100086 Instruction Manual, 100 bp Molecular Ruler Click to download
LIT530 Instruction Manual, AmpliSize Molecular Ruler, Rev D Click to download
4100085 Instruction Manual, 20 bp Molecular Ruler, Rev C Click to download
4006137 Instruction Manual, EZ Load Molecular Rulers Click to download
2121 User Guide to Nucleic Acid Standards Bulletin, Rev D Click to download
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]