Macro-Prep t-Butyl HIC Media

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Overview

Specifications
  Methyl HIC Support t-Butyl HIC Support
Functional group -CH3 -C(CH3)3
Nominal particle size 50 µm 50 µm
Binding capacity* >25 mg/ml >15 mg/ml
Recommended maximum linear flow rate 3,000 cm/hr 3,000 cm/hr
pH stability 1–12 1–12
Regeneration 70% ethanol 70% ethanol
Autoclavability (121°C, 30 min) Yes Yes
Storage 20% ethanol 20% ethanol

* Determined with human serum albumin.

Chemical Structure of Macro-Prep HIC Media

Macro-Prep HIC: Macro-Prep t-butyl contains t-butyl and carboxyl groups; Macro-Prep methyl contains methyl and carboxyl groups.

Macro-Prep HIC Media
  Retention Times (min)
PeakProteinMethylt-Butyl
1Cytochrome c7.310.41
2Ovalbumin32.2540.22
3Α-Anylase62.8171.5
4Ferritin81.4990.9

Comparison of retention times using Macro-Prep methyl and t-butyl ligands.

Macro-Prep HIC Dynamic Binding Capacity

Macro-Prep HIC supports can be sanitized extensively with .15% peracetic acid.

Retention Times of Macro-Prep HIC Media

Macro-Prep HIC supports can be sanitized extensively with .15% peracetic acid.

Macro-Prep hydrophobic interaction chromatography (HIC) media are methacrylate-based, 50 µm beads used for protein, polypeptide, enzyme, and nucleic acid purification. They are designed specifically for intermediate purification steps that remove host-cell contaminants from partially purified targets. Due to their rigidity and unique surface chemistry, these media are particularly suited for HIC operations requiring high throughput and high recovery of target.

These media are available in two functional forms:

  • Macro-Prep methyl HIC support — weakly hydrophobic, for purifying compounds that have strong hydrophobic regions
  • Macro-Prep t-butyl HIC support — mildly hydrophobic, for purifying compounds that have few or weakly hydrophobic regions

Both media are chemically and thermally stable. They are autoclavable and can withstand treatment in acid, chaotropic agents, or detergents while retaining high protein-binding capacities.

Macro-Prep t-butyl HIC support operates on a mechanism of interaction that is based on hydrophobicity and charge. The t-butyl groups are mildly hydrophobic. Depending on the pH of loading and elution buffers, the carboxyl groups can be exploited to ionically repel target molecules while the hydrophobic groups retain contaminants. This is an ideal strategy that minimizes the product loss commonly experienced with HIC media due to denaturation of proteins upon exposure to hydrophobic surfaces.

For more information, request bulletin 1841.

Regulatory support files are available upon request. Bio-Rad laboratories is an ISO 9001-registered corporation.

Macro-Prep t-Butyl HIC Support

158-0090
25 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $125.00
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Macro-Prep t-Butyl HIC Support

156-0090
100 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $473.00
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Macro-Prep t-Butyl HIC Support

156-0091
500 ml, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

List Price:   $1,824.00
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Macro-Prep t-Butyl HIC Support

156-0092
5 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

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Macro-Prep t-Butyl HIC Support

156-0093
10 L, t-butyl hydrophobic interaction chromatography media, 50 μm particle size

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Number Description Options
1841 Macro-Prep HIC Support Product Information Sheet, Rev E Click to download
LIT486 Instruction Manual, Macro-Prep HIC Supports, Rev C Click to download [ Add to Cart (Free) ]
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download