Macro-Prep® High S Resin

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Overview

Effect of Flow Rate

Effect of flow rate on separation and peak symmetry when using Macro-Prep High S media. A 5 ml sample (1.4 mg/ml) of myoglobin (peak 1), ribonuclease A (peak 2), and cytochrome c (peak 3) was run on 1 x 13 cm (8.7 ml) column at each of the indicated flow rates. The buffer was 20 mM potassium phosphate, pH 8.0 with a gradient of 0–1 M KCI over 75 ml.


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Macro-Prep High S Resin is a strong cation exchange resin containing sulfonate functional groups that is ideal for purifying basic and neutral proteins and peptides. It is an excellent choice for rapid purification that meets the demands of analytical, semipreparative, and process-scale applications.

This methacrylate copolymer bead provides high-resolution separations at very high flow rates. Changes in pH or ionic strength of the buffer do not cause shrinking or swelling of the support. The superior mechanical and chemical stability of Macro-Prep High S Resin make it a preferred choice over other sulfonate cation exchangers on the market. Macro-Prep High S Resin can be sanitized with chaotropic agents and can be autoclaved. It provides consistent and reproducible results.

Features and Benefits of Macro-Prep High S Resin

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • High chemical, mechanical, and thermal stability

More Information

Macro-Prep® High S Resin Product Information Sheet (Bulletin 5643)

Instruction Manual, Macro-Prep® Ion Exchange Resins (LIT271)

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Properties of Macro-Prep High S Support  
Type of support Strong cation exchange
Functional group –SO3-
Typical dynamic binding capacity* ≥49 lgG/ml
Nominal particle size 50 µm
Nominal pore diameter 1,000 Å
Recommended maximum linear flow rate 3,000 cm/hr
Autoclavability 30 min at 121°C
pH stability 1–10
Regeneration in 1–2 M NaCl/KCI or 70% ethanol
*Determined with human lgG.  
Chemical Compatability of Macro-Prep High S Support  
1% SDS Yes
8 M guanidine-HCI Yes
1 N HCI Yes
100% ethanol Yes
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Macro-Prep High S Support #156-0031
Macro-Prep High S Support

1560030
100 ml, strong cation exchange chromatography media, 50 μm particle size

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$220.00
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Macro-Prep High S Support #156-0032
Macro-Prep High S Support

1560031
500 ml, strong cation exchange chromatography media, 50 μm particle size

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$840.00
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Macro-Prep High S Support #156-0033
Macro-Prep High S Support

156-0032
5 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Macro-Prep High S Support

156-0033
10 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Macro-Prep High S Support

1580030
25 ml, strong cation exchange chromatography media, 50 μm particle size

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$69.00
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Number Description Options
5643 Macro-Prep® High S Support Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
1985 Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B. Click to download [ Add to Cart (Free) ]
1913 Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Supports (Poster), Rev B Click to download
LIT271 Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D Click to download
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
2026 Evaluation of Different Approaches for the Chromatographic Purification of Monoclonal Antibodies Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download
2079 Non-Protein A Based Purification of MAb 414, Rev B Click to download